Afdn (NM_010806) Mouse Tagged ORF Clone

CAT#: MG226019

Mllt4 (GFP-tagged) - Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog Drosophila); translocated to 4 (Mllt4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Afdn
• Synonyms: 5033403D15Rik; Af-6; AF6; Afadin; Gm314; I-afadin; Mllt4; S-afadin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226019 representing NM_010806
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGGCGGCCGCGACGAAGAGCGGCGGAAGCTGGCCGACATCATTCACCACTGGAACGCCAACC
  GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTTCATGGAGTCATGAGATTTTATTT
  TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGAGTCTCTAGCACAGCCACCACTCAAGAT
  GTGATTGAAACACTGGCAGAGAAGTTCCGCCCTGACATGCGCATGCTCTCCTCTCCCAAGTACTCCCTCT
  ATGAAGTGCATGTCAGTGGAGAAAGAAGATTGGACATTGATGAGAAACCTCTAGTTGTACAGTTGAATTG
  GAACAAAGATGATCGAGAGGGTCGATTCGTCCTTAAGAACGAGAATGACGCCATTCCTGCCAAGAAGGCT
  CAGAGTAATGGACCTGAAAAGCAGGAGAAAGAAGGTGTTATTCAGAACTTCAAAAGAACTCTCTCAAAGA
  AAGAAAAGAAGGAAAAAAAGAAGAAAGAGAAGGAGGCATTGAGACAAGCCTCTGATAAGGAAGAAAGGCC
  TTCTCAAGGGGATGACAGTGAGAACTCACGACTGGCTGCTGAAGTTTACAAAGACATGCCTGAGACCAGC
  TTTACTAGGACCATTTCTAACCCTGAAGTTGTCATGAAACGGCGGCGGCAGCAAAAACTGGAGAAGAGAA
  TGCAAGAGTTTCGGAGCTCAGATGGGCGGCCTGACTCAGGTGGAACACTGAGGATATATGCAGATAGTTT
  AAAACCAAATATTCCATACAAGACAATCCTGCTGTCTACTACCGATACCGCAGACTTTGCTGTAGCCGAA
  TCTTTGGAGAAGTATGGTCTGGAAAAAGAGAATCCCAAGGACTACTGTATTGCCCGGGTTATGCTTCCTC
  CTGGAGCCCAGCATTCTGATGAGAGAGGTGCTAAGGAAATAATTCTTGACGATGATGAATGTCCTTTACA
  AATCTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGACCACCGGACTAC
  ATCCCAAAGAAAATGAAGAAACATGTTGAAGGGAAGTCGTTGAAGGGGAAAGACAGAGCTGACGGGTCTG
  GCTACGGCTCCGCTCTTCCTCCCGAGAAGCTGCCCTACTTAGTAGAGCTAAGCCCAGGGAGAAGGAATCA
  CTTTGCCTACTACAGCTATCACACTTACGAAGATGGCTCTGACTCCAGAGACAAGCCAAAGTTATACCGC
  CTTCAATTAAGTGTGACTGAAGTTGGGACAGAGAAATTTGATGACAATTCTATCCAGCTGTTTGGCCCCG
  GAATTCAGCCCCATCACTGTGATCTCACCAACATGGATGGAGTGGTCACTGTGACACCCAGAAGTATGGA
  TGCAGAGACCTACGTGGATGGTCAGCGCATCTCAGAGACCACAATGCTGCAGAGTGGCATGAGACTGCAG
  TTTGGCACCTCGCATGTGTTTAAGTTTGTGGACCCCATCCAGGACCACGTTCTTTCCAAGAGATCTGTGG
  ATGGAGGCCTGATGGTCAAGGGCCCAAGACATAAACCTGGAGCTGTTCAGGAGACAACCTTTGAATTGGG
  AGGAGATGTCCACAGTGGGACAGCATTGCCGGCAAGCAGGAGCACTACTAGACTGGACAGTGACAGGGTG
  TCCTCTGCCTCTAGCACAGCTGAGCGAGGGATGGTGAAGCCGATGATCCGACTGGACCAGGAGCAGGAAT
  ATCGACGACGAGAAAACAGGACTCAGGATGCTACTGGGCCTGAACTGATACTGCCTGCCAGCATTGAATT
  CAGGGAAAGCTCTGAAGACTCTTTTTTATCTGCCATTATAAACTATACCAATAGTTCTACAGTTCATTTT
  AAGTTGTCACCTACATATGTGTTATACATGGCATGCCGGTATGTATTGTCCAGCCAGCACAGGCCCGACA
  TCAGTCCTACAGAGCGTACACATAAGGCCATTGCTGTTGTCAACAAGATGGTGAGCATGATGGAAGGGGT
  CATCCAGAAACAGAAGAACATTGCAGGAGCACTTGCTTTTTGGATGGCAAATGCATCTGAGCTTCTCAAC
  TTCATCAAGCAGGACCGAGACCTTAGTCGGATCACGCTGGATGCCCAGGATGTCCTAGCACACTTGGTTC
  AGATGGCATTTAAGTACTTGGTTCATTGCCTTCAGTCAGAACTTAATAACTACATGCCAGCCTTTCTGGA
  TGATCCTGAGGAGAACAGTCTGCAAAGACCAAAAATAGATGATGTGCTGCACACACTCACAGGAGCCATG
  TCCTTGCTGCGGCGCTGCAGAGTCAATGCTGCCTTGACCATCCAGCTCTTCTCTCAGCTTTTCCACTTCA
  TCAATATGTGGCTGTTCAACAGACTGGTGACTGACCCAGATTCAGGCCTGTGCTCCCACTACTGGGGTGC
  CATTATCCGCCAGCAGCTGGGGCATATTGAAGCGTGGGCAGAGAAGCAGGGGCTAGAGCTGGCAGCTGAC
  TGTCACTTGAGCAGAATTGTACAGGCTACAACATTGCTTACGATGGATAAGTATGTACCTGATGACATTC
  CAAATATAAACAGCACATGCTTTAAGTTAAATTCTCTGCAGCTTCAAGCCTTGTTACAAAATTACCACTG
  TGCACCCGATGAGCCTTTTATTCCTACGGATCTCATAGAAAATGTTGTGGCTGTTGCTGAGAACACAGCT
  GATGAGCTCGCCCGCAGTGATGGCAGAGACGTGCAGCTGGAGGAAGACCCAGACCTACAGCTGCCTTTTC
  TTCTGCCAGAAGATGGCTATTCCTGTGATGTTGTCAGAAACATTCCAAATGGTTTACAAGAATTTTTAGA
  CCCTCTGTGCCAGAGAGGATTTTGTAGATTGGTTCCTCACACACGTTCACCAGGTACCTGGACAATATAT
  TTTGAAGGAGCAGATTATGAAAGTCACCTTATGCGTGAGAACGCAGAACTGGCCCAGCCTCTGAGGAAGG
  AGCCTGAGATAATCACTGTGACTCTGAAGAAGCAGAATGGAATGGGCCTCAGCATCGTTGCAGCCAAGGG
  TGCTGGCCAGGATAAACTGGGAATCTACGTTAAGTCGGTTGTCAAAGGAGGTGCTGCAGATGTGGATGGA
  CGATTAGCTGCTGGTGACCAACTCCTCAGTGTGGATGGACGAAGTCTGGTTGGACTTTCTCAGGAAAGGG
  CAGCAGAACTCATGACAAGAACCAGTTCCGTGGTTACACTGGAAGTTGCAAAGCAAGGTGCCATCTATCA
  CGGCCTGGCCACCCTCCTTAACCAGCCGTCCCCCATGATGCAAAGAATTTCAGATCGTCGTGGCTCAGGT
  AAACCCAGACCAAAGAGTGAAGGTTTTGAGCTCTATAATAATTCAGCTCAAAATGGCTCCCCAGAGAGTC
  CTCAGATGCCTTGGACAGAGTACAGTGAGCCAAAGAAACTGCCTGGGGATGACAGGCTGATGAAAAACAG
  AGCGGACCACCGTTCCAGCCCCAATGTGGCAAATCAGCCTCCTAGCCCTGGAGGGAAGGGTCCATATACA
  TCTGGAACAGCAGCTAAGATAACATCTGTCTCCACTGGAAACCTCTGCACTGAGGAGCAGTCCCCTCCAC
  CCAGACCTGAAGCCTATCCTATCCCTACTCAGACATACACCAGAGAGTATTTTACCTTTCCAGCTTCAAA
  GTCGCAGGATCGGATGGCTCCTCCTCAGAGCCAGTGGCCAAATTATGAGGAAAAACCACATGTGCACACA
  GAAAGTAATCATTCCAGTATTGCAATCCAGCGTGTTACCCGTTCCCAGGAAGAGCTTCGGGAAGAAAAGG
  TTTACCAACTTGAGCGGCATCGAGTAGAGGCAGGCATGGATCGCAAGTGTGACAGTGATATGTGGATCAA
  TCAGAGCTCCTCAGTGGAATCCAGCACATCCAGCCAGGAACACCTGAACCATTCTTCCAAGTCAGTTACC
  CCTGCTTCCACTCTGACCAAGAGTGGTCCTGGCCGTTGGAAAACACCAGCAGCGGTGCTGCCCACCCCTG
  TGGCTGTCTCTCAGCCCATTCGAACAGACCTCCCTCCACCACCACCTCCACCGCCGGTCCACTACACCAG
  TGAGTTTGATGGTATTCCAATGGATTTGCCCCTCCCACCTCCTCCTGCCAACCAGGCAGGACCCCAGTCT
  GCTCAGGTGGCTGCTGCAGAGTGGAAAAAGAGAGAGGAGCACCAGAGGTGGTATGAGAAAGAGAAGGCCC
  GCCTGGAGGAAGAACGAGAGAGAAAGCGCAGGGAGCAGGAGCGGAAGTTGGGGCAGATGCGCTCTCAGAC
  CCTGAACCCTGCTTCCTTCTCACCTTTGGCCACACAGGCCAAGCCAGAAAAGCCTTCCACACTGCAGAGG
  CCCCAAGAAACAGTCATTCGGGAGCTTCAGCCCCAGCAGCAGCCCCGCACGATCGAGCGCAAGGATCTGC
  AGTATATAACCATCAGTAAAGAGGAGCTGTCCTCTGGTGATAGTCTGTCTCCAGACCCCTGGAAACGAGA
  TGCCAGGGAGAAGCTGGAGAAGCAACAGCAAATGCACATTGTGGACATGCTAAGCAAGGAGATCCACGAG
  CTGCAAAACAAGGTGGACCGTACTGCGGAGGAGAGTGACCGCCTGCGGAAGCTTATGCTGGAGTGGCAGT
  TCCAGAAGAGACTACAGGAGTCCAAACAGAAGGATGAGGATGATGACGAAGAGGAAGATGATGATGTGGA
  CACCATGCTGATCATGCAGCGTCTGGAGGCCGAGCGGAGAGCCAGGATGCAGGATGAGGAGCGCAGGCGC
  CAGCAACAGTTGGAAGAGATGCGGAAACGAGAAGCAGAAGATCGAGTAAGACAAGAGGAAGATGGACGCC
  ATCAGGAGGAGGAGCGAGTAAAGAGAGATGCTGAAGAAAAGAGGCGACAGGAGGAAGGGTATTACAGCCG
  CCTAGAAGCTGAGAGGCGCCGACAGCACGAAGAGGCAGCACGCAGGCTGCTGGAGCCTGAAGAGCCCGGG
  CTGAGCCGACCTCCACTTCCACGGGACTATGAGCCCCCGTCCTTGTCCTCAGCACCCTGTGCCCCTCCTC
  CCCCACCTCAGCGAAACGCATCCTACCTCAAAACACAGGTCCTCTCCCCAGACTCGCTGTTCACTGCCAA
  GTTTGTTGCGTATGATGAGGAGGAGGAGGACTACGGCCCAGCAGGACCAAACTCTTACTCGGGATCTGCA
  GGCACAGCTGTGGGAGCCTATGATGCCCCTCGGGAAGCGAGAGAGAAACTGACTAGGAGCCAAGACGCAG
  ACTTACCTGGCAGTTCTGGAGCCCCTGAAAATCTGACATTTAAAGAGCGTCAGCGCCTTTTTTCACAAGG
  GCAGGATGTGTCTGACAAAGTGAAAGCTTCTCGTAAATTAACAGAGCTCGAGAATGAACTGAACACAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226019 representing NM_010806
  Red=Cloning site Green=Tags(s)
  
  MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQD
  VIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKA
  QSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETS
  FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAE
  SLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDY
  IPKKMKKHVEGKSLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYR
  LQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQ
  FGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDVHSGTALPASRSTTRLDSDRV
  SSASSTAERGMVKPMIRLDQEQEYRRRENRTQDATGPELILPASIEFRESSEDSFLSAIINYTNSSTVHF
  KLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLN
  FIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAM
  SLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAAD
  CHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTA
  DELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIY
  FEGADYESHLMRENAELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDG
  RLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSG
  KPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKGPYT
  SGTAAKITSVSTGNLCTEEQSPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQSQWPNYEEKPHVHT
  ESNHSSIAIQRVTRSQEELREEKVYQLERHRVEAGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVT
  PASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPVHYTSEFDGIPMDLPLPPPPANQAGPQS
  AQVAAAEWKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRSQTLNPASFSPLATQAKPEKPSTLQR
  PQETVIRELQPQQQPRTIERKDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHE
  LQNKVDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARMQDEERRR
  QQQLEEMRKREAEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPG
  LSRPPLPRDYEPPSLSSAPCAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDEEEEDYGPAGPNSYSGSA
  GTAVGAYDAPREAREKLTRSQDADLPGSSGAPENLTFKERQRLFSQGQDVSDKVKASRKLTELENELNTK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010806
• ORF Size: 5460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010806.1, NP_034936.1
• RefSeq Size: 7401
• RefSeq ORF: 5463
• Locus ID: 17356