Pbx1 (NM_008783) Mouse Tagged ORF Clone

CAT#: MG226070

Pbx1 (GFP-tagged) - Mouse pre B-cell leukemia transcription factor 1 (Pbx1) transcript variant b, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pbx1
• Synonyms: 2310056B04Rik; D230003C07Rik; Pbx-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226070 representing NM_008783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGCAGCCGAGGCTGATGCATTCCCACGCTGGGGTCGGGATGGCCGGACACCCCGGCCTGTCCC
  AGCACTTGCAGGATGGGGCCGGAGGGACCGAGGGGGAGGGCGGGAGGAAGCAGGACATCGGGGACATTTT
  ACAGCAAATTATGACCATCACAGACCAGAGTTTGGATGAAGCGCAGGCCAGAAAACATGCTTTAAACTGC
  CACAGAATGAAGCCTGCCTTGTTTAATGTGTTGTGTGAAATCAAAGAAAAAACAGTTTTGAGTATTCGGG
  GAGCCCAAGAAGAGGAGCCCACAGACCCCCAGCTCATGCGACTGGACAACATGCTGCTAGCAGAAGGGGT
  GGCGGGGCCTGAGAAGGGCGGAGGCTCGGCAGCGGCGGCGGCGGCAGCGGCAGCTTCTGGGGGTGCAGGT
  TCAGACAACTCAGTGGAGCATTCCGACTACAGAGCCAAACTCTCACAGATCAGACAAATCTACCACACAG
  AGCTGGAGAAGTATGAGCAGGCATGCAATGAATTCACCACCCACGTGATGAACCTCCTTCGAGAGCAAAG
  CCGGACCAGGCCCATCTCTCCGAAGGAGATCGAGCGGATGGTGAGCATCATCCACCGCAAGTTCAGCTCC
  ATCCAGATGCAGCTGAAACAGAGCACGTGCGAGGCCGTCATGATCCTGCGCTCCCGGTTCCTGGATGCGA
  GGCGGAAGAGACGGAATTTCAACAAGCAAGCCACAGAAATTCTGAATGAATATTTCTATTCCCATCTCAG
  CAACCCTTACCCCAGTGAGGAAGCCAAAGAGGAGTTAGCCAAGAAGTGCGGCATCACAGTCTCCCAGGTA
  TCAAACTGGTTTGGAAATAAGCGAATCCGGTACAAGAAGAACATAGGTAAATTTCAAGAGGAAGCCAATA
  TTTATGCTGCCAAAACGGCTGTCACAGCCACCAATGTGTCAGCCCATGGAAGCCAAGCTAACTCGCCCTC
  TACTCCCAACTCAGCGGGTGGATACCCTTCGCCATGTTATCAGCCAGACAGGAGGATACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226070 representing NM_008783
  Red=Cloning site Green=Tags(s)
  
  MDEQPRLMHSHAGVGMAGHPGLSQHLQDGAGGTEGEGGRKQDIGDILQQIMTITDQSLDEAQARKHALNC
  HRMKPALFNVLCEIKEKTVLSIRGAQEEEPTDPQLMRLDNMLLAEGVAGPEKGGGSAAAAAAAAASGGAG
  SDNSVEHSDYRAKLSQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVSIIHRKFSS
  IQMQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQV
  SNWFGNKRIRYKKNIGKFQEEANIYAAKTAVTATNVSAHGSQANSPSTPNSAGGYPSPCYQPDRRIQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008783
• ORF Size: 1041 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008783.2, NP_032809.1
• RefSeq Size: 3978
• RefSeq ORF: 1044
• Locus ID: 18514
• Gene Summary: This gene encodes a homeobox protein that belongs to the three-amino-acid loop extension/Pre-B cell leukemia transcription factor (TALE/PBX) family of proteins. The encoded protein is involved in several biological processes during embryogenesis including steroidogenesis, sexual development and the maintenance of hematopoietic stem cells. This protein functions in the development of several organ systems and plays a role in skeletal patterning and programming. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]