Ptger4 (NM_008965) Mouse Tagged ORF Clone

CAT#: MG226174

Ptger4 (GFP-tagged) - Mouse prostaglandin E receptor 4 (subtype EP4) (Ptger4) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptger4
• Synonyms: EP4; Ptgerep4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226174 representing NM_008965
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGTTGGAGGTACCATTCCTAGATCGAACCGTGAGCTCCAACGCTGTGTGTTACTAACCACCA
  CCATCATGTCCATCCCCGGAGTCAACGCGTCCTTCTCCTCCACTCCGGAGAGGCTGAACAGCCCGGTGAC
  CATTCCCGCAGTGATGTTCATCTTCGGGGTGGTGGGCAACCTGGTGGCCATCGTAGTATTGTGCAAGTCG
  CGCAAGGAGCAGAAAGAGACGACCTTTTACACTCTAGTATGTGGGCTGGCTGTCACTGACCTTCTGGGCA
  CCTTGTTGGTAAGCCCGGTGACCATCGCCACATACATGAAGGGCCAGTGGCCCGGAGACCAGGCACTGTG
  TGACTATAGCACCTTCATCCTACTTTTCTTCGGTCTGTCGGGTCTCAGCATCATCTGTGCCATGAGCATC
  GAGCGCTACCTGGCCATCAACCACGCCTACTTCTACAGCCACTACGTGGACAAGCGGCTGGCCGGCCTCA
  CACTCTTCGCCATCTATGCATCTAACGTGCTGTTCTGCGCGCTGCCCAACATGGGCCTGGGCAGATCCGA
  GCGGCAGTACCCGGGCACCTGGTGCTTCATCGACTGGACCACCAACGTAACGGCCTACGCCGCCTTCTCT
  TACATGTACGCCGGCTTCAGCTCCTTCCTCATCCTTGCCACCGTGCTCTGCAACGTGCTGGTGTGCGGCG
  CGCTGCTCCGCATGCACCGCCAGTTCATGCGCCGCACCTCGTTGGGCACGGAGCAGCACCATGCGGCTGC
  CGCCGCCGCGGTAGCTTCGGTGGCCTGTCGGGGCCACGCTGGGGCCTCCCCAGCCCTGCAGCGCCTCAGC
  GACTTTCGCCGCCGCAGGAGTTTCCGGCGCATCGCGGGTGCGGAGATCCAGATGGTCATCTTACTCATCG
  CCACCTCTCTGGTGGTGCTCATCTGCTCCATTCCGCTCGTGGTGCGAGTGTTCATTAACCAGTTATATCA
  GCCAAACGTGGTGAAAGACATCAGCAGAAACCCAGATTTGCAGGCCATCAGGATTGCTTCTGTGAACCCC
  ATCCTGGACCCCTGGATTTACATCCTTCTTCGGAAGACTGTGCTCAGTAAAGCCATAGAGAAGATCAAGT
  GCCTCTTCTGCCGCATTGGCGGTTCCGGCAGAGACAGCTCGGCCCAGCACTGCTCAGAGAGTCGGAGGAC
  ATCTTCCGCCATGTCCGGCCACTCTCGCTCCTTCCTCGCCCGGGAGTTAAAGGAGATCAGCAGCACGTCC
  CAGACCCTCCTGTACCTGCCAGACCTGACTGAAAGCAGCCTCGGAGGCAGGAATTTGCTTCCAGGTTCGC
  ATGGCATGGGCCTGACCCAAGCAGACACCACCTCGCTGAGAACTTTGCGAATTTCCGAGACCTCAGACTC
  CTCCCAGGGCCAGGACTCTGAGAGTGTCCTGTTGGTGGATGAGGTTAGTGGGAGCCACAGAGAGGAGCCT
  GCCTCTAAAGGAAACTCTCTGCAAGTCACATTCCCCAGTGAAACTCTGAAATTATCTGAAAAATGTATA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226174 representing NM_008965
  Red=Cloning site Green=Tags(s)
  
  MAEVGGTIPRSNRELQRCVLLTTTIMSIPGVNASFSSTPERLNSPVTIPAVMFIFGVVGNLVAIVVLCKS
  RKEQKETTFYTLVCGLAVTDLLGTLLVSPVTIATYMKGQWPGDQALCDYSTFILLFFGLSGLSIICAMSI
  ERYLAINHAYFYSHYVDKRLAGLTLFAIYASNVLFCALPNMGLGRSERQYPGTWCFIDWTTNVTAYAAFS
  YMYAGFSSFLILATVLCNVLVCGALLRMHRQFMRRTSLGTEQHHAAAAAAVASVACRGHAGASPALQRLS
  DFRRRRSFRRIAGAEIQMVILLIATSLVVLICSIPLVVRVFINQLYQPNVVKDISRNPDLQAIRIASVNP
  ILDPWIYILLRKTVLSKAIEKIKCLFCRIGGSGRDSSAQHCSESRRTSSAMSGHSRSFLARELKEISSTS
  QTLLYLPDLTESSLGGRNLLPGSHGMGLTQADTTSLRTLRISETSDSSQGQDSESVLLVDEVSGSHREEP
  ASKGNSLQVTFPSETLKLSEKCI
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008965
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008965.1
• RefSeq Size: 2442
• RefSeq ORF: 1542
• Locus ID: 19219
• Gene Summary: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. [UniProtKB/Swiss-Prot Function]