Ccne1 (NM_007633) Mouse Tagged ORF Clone

CAT#: MG226185

Ccne1 (GFP-tagged) - Mouse cyclin E1 (Ccne1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccne1
• Synonyms: AW538188; CycE1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226185 representing NM_007633
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGGGAGAGAGACTCGACGGACCACAGCAACATGAAAGAAGAAGGTGGCTCCGACCTTTCAGTCC
  GCTCCAGAAAAAGGAAGGCAAATGTGGCCGTGTTTTTGCAAGACCCAGATGAAGAAATTGCCAAGATTGA
  CAAGACTGTGAAAAGCGAGGATAGCAGTCAGCCCTGGGATGATAATTCAGCATGCGTGGACCCCTGCTCT
  TTCATCCCCACCCCTAACAAAGAAGAGGACAATGAGCTTGAATACCCCAGGACTGCATTTCAGCCTCGGA
  AAATCAGACCACCCAGAGCCTCCCCACTTCCCGTCTTGAATTGGGGCAATAGAGAAGAGGTTTGGAGGAT
  CATGTTAAACAAAGAAAAGACTTACCTGAGAGATGAGCACTTTCTGCAGCGTCATCCTCTCCTGCAGGCG
  AGGATGAGAGCAGTTCTTCTGGATTGGCTAATGGAGGTGTGCGAAGTCTATAAGCTCCACAGAGAGACGT
  TCTACTTGGCACAGGACTTCTTTGATCGTTACATGGCATCACAACATAATATCATAAAAACACTTTTACA
  GCTTATTGGGATTTCAGCCTTATTTATTGCTTCAAAACTTGAGGAAATCTACCCTCCAAAGTTGCACCAG
  TTTGCTTATGTTACAGATGGCGCTTGCTCCGGGGATGAAATTCTTACCATGGAATTGATGATGATGAAGG
  CCCTTAAGTGGCGTCTAAGCCCTCTGACCATTGTGTCCTGGCTGAATGTCTATGTCCAAGTGGCCTATGT
  CAACGACACGGGTGAGGTGCTGATGCCTCAGTACCCACAGCAGGTCTTCGTGCAGATCGCAGAGCTTCTA
  GACCTGTGCGTCCTGGATGTTGGCTGCTTAGAATTTCCTTATGGTGTCCTCGCTGCTTCTGCTTTGTATC
  ATTTCTCCTCACTGGAGTTGATGCAGAAGGTCTCAGGTTATCAGTGGTGCGACATAGAGAAGTGTGTCAA
  ATGGATGGTTCCGTTCGCCATGGTTATCCGGGAGATGGGAAGTTCCAAGCTCAAGCACTTCCGGGGAGTC
  CCCATGGAAGACTCCCACAACATCCAGACCCACACCAACAGCTTGGATTTGCTGGACAAAGCCCAAGCAA
  AGAAAGCCATATTGTCAGAACAGAATAGGATTTCTCCTCCTCCGAGTGTGGTCCTGACACCCCCACCCAG
  CAGTAAGAAGCAGAGCAGCGAGCAGGAGACAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226185 representing NM_007633
  Red=Cloning site Green=Tags(s)
  
  MPRERDSTDHSNMKEEGGSDLSVRSRKRKANVAVFLQDPDEEIAKIDKTVKSEDSSQPWDDNSACVDPCS
  FIPTPNKEEDNELEYPRTAFQPRKIRPPRASPLPVLNWGNREEVWRIMLNKEKTYLRDEHFLQRHPLLQA
  RMRAVLLDWLMEVCEVYKLHRETFYLAQDFFDRYMASQHNIIKTLLQLIGISALFIASKLEEIYPPKLHQ
  FAYVTDGACSGDEILTMELMMMKALKWRLSPLTIVSWLNVYVQVAYVNDTGEVLMPQYPQQVFVQIAELL
  DLCVLDVGCLEFPYGVLAASALYHFSSLELMQKVSGYQWCDIEKCVKWMVPFAMVIREMGSSKLKHFRGV
  PMEDSHNIQTHTNSLDLLDKAQAKKAILSEQNRISPPPSVVLTPPPSSKKQSSEQETE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007633
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007633.2, NP_031659.2
• RefSeq Size: 2022
• RefSeq ORF: 1227
• Locus ID: 12447
• Gene Summary: Essential for the control of the cell cycle at the G1/S (start) transition. [UniProtKB/Swiss-Prot Function]