Smurf1 (NM_001038627) Mouse Tagged ORF Clone

CAT#: MG226199

Smurf1 (GFP-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smurf1
• Synonyms: 4930431E10Rik; mKIAA1625
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226199 representing NM_001038627
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAACCCCGGGACCCGTAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTATTGTGTGCCAAGA
  ACCTTGCAAAGAAAGACTTCTTCAGACTCCCCGACCCCTTTGCCAAGATTGTTGTGGACGGCTCTGGGCA
  GTGCCACTCAACCGACACTGTGAAAAACACCCTGGACCCAAAGTGGAACCAGCACTATGACCTGTATGTT
  GGGAAAACGGACTCGATAACCATCAGTGTGTGGAACCACAAGAAGATCCACAAGAAGCAGGGGGCTGGCT
  TCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTGAAAGACACTGGCTACCAGCGTTTGGA
  TCTATGCAAACTAAATCCCTCAGATACTGATGCAGTTCGTGGCCAAATAGTGGTCAGTTTACAGACCCGA
  GACAGAATAGGCGGTGGAGGGTCAGTGGTGGACTGCAGAGGGCTGCTGGAGAACGAAGGAACAGTGTATG
  AAGACTCAGGCCCTGGAAGGCCGCTCAGCTGCCTCATGGAGGAACCTGCCCCATATACAGATGGTACTGG
  TGCAGCAGCAGGAGGCGGGAACTGCAGGTTTGTGGAGTCTCCAAGCCAAGATCAGAGACTCCTGGTACAG
  CGACTCCGAAATCCTGAGGTTCGAGGGCCCTTACAGACACCCCAGAACCGACCACATGGCCACCAGTCGC
  CAGAGCTGCCTGAAGGCTATGAGCAAAGGACAACAGTGCAGGGACAAGTTTACTTTTTGCACACGCAGAC
  TGGAGTCAGTACATGGCATGACCCCAGGATCCCCAGAGACCTTAACAGTGTGAACTGCGATGAACTTGGG
  CCACTGCCTCCAGGCTGGGAAGTCCGAAGCACAGTGTCGGGAAGAATCTATTTTGTAGATCACAATAATA
  GGACAACCCAGTTTACAGATCCACGGCTTCACCACATCATGAATCACCAGTGCCAACTCAAGGAGCCCAG
  CCAGCCGCTGCAGCTGCCCAGTGAGGGCTCCGTGGAGGACGAGGAGCTTCCTGCCCAGAGATATGAGAGG
  GACTTAGTCCAGAAGCTCAAAGTTCTCAGGCACGAGCTCTCTCTTCAGCAGCCCCAGGCTGGTCACTGTC
  GAATAGAAGTCTCCAGAGAAGAGATATTTGAGGAGTCGTATCGCCAGATCATGAAGATGCGGCCAAAAGA
  CCTAAAGAAGCGCCTGATGGTGAAGTTCCGAGGGGAGGAAGGTTTGGACTATGGTGGAGTGGCTCGGGAG
  TGGCTGTATTTGTTGTGCCATGAAATGTTGAACCCGTACTATGGACTCTTCCAGTATTCCACGGACAATA
  TTTACACACTGCAGATCAACCCAGATTCTTCTATCAACCCTGACCATCTGTCATACTTCCACTTTGTGGG
  TCGCATCATGGGTCTGGCTGTGTTCCACGGACACTACATAAATGGGGGTTTCACAGTTCCGTTCTACAAG
  CAGCTCCTGGGGAAGCCAATCCAGCTGTCGGACCTGGAGTCCGTGGACCCAGAACTGCATAAGAGCTTGG
  TGTGGATTCTAGAGAATGACATCACGCCTGTGTTGGATCATACCTTCTGCGTGGAGCACAACGCTTTCGG
  GCGGATTCTCCAGCATGAACTGAAACCCAATGGCAGAAATGTGCCTGTCACTGAGGAGAACAAGAAGGAA
  TACGTCCGGCTGTATGTGAACTGGAGGTTTATGAGAGGAATCGAAGCCCAGTTCTTAGCACTTCAGAAGG
  GGTTTAACGAACTCATTCCCCAACACTTGCTGAAGCCCTTTGACCAGAAGGAACTAGAGCTGATAATAGG
  TGGGCTGGATAAGATAGACCTGAACGACTGGAAGTCCAACACCCGGCTGAAACACTGTGTGGCAGACAGC
  AACATCGTCAGGTGGTTCTGGCAGGCGGTGGAGACCTTCGATGAGGAGAGGAGAGCCAGACTCCTGCAGT
  TTGTGACAGGATCCACAAGAGTTCCTCTCCAAGGCTTCAAGGCTCTGCAAGGCTCTACAGGCGCGGCAGG
  GCCCCGGCTGTTCACCATTCACCTGATAGACGCCAATACAGACAACCTGCCCAAGGCCCATACCTGCTTT
  AATCGGATCGACATCCCACCCTATGAGTCCTATGAGAAGCTCTATGAGAAGCTGCTGACAGCGGTGGAGG
  AAACCTGTGGCTTTGCAGTGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226199 representing NM_001038627
  Red=Cloning site Green=Tags(s)
  
  MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYV
  GKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTR
  DRIGGGGSVVDCRGLLENEGTVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQ
  RLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELG
  PLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQRYER
  DLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVARE
  WLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYK
  QLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKE
  YVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADS
  NIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCF
  NRIDIPPYESYEKLYEKLLTAVEETCGFAVE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001038627
• ORF Size: 2193 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038627.1, NP_001033716.1
• RefSeq Size: 5333 bp
• RefSeq ORF: 2196 bp
• Locus ID: 75788
• Cytogenetics: 5 G2