Plcb1 (NM_019677) Mouse Tagged ORF Clone

CAT#: MG226225

Plcb1 (GFP-tagged) - Mouse phospholipase C beta 1 (Plcb1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plcb1
• Synonyms: 3110043I21Rik; AI132408; mKIAA0581; Plcb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226225 representing NM_019677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGCTCAGCCCGGAGTGCACGCCTTGCAACTCAAGCCCGTGTGCGTGTCCGACAGCCTCAAGA
  AGGGCACCAAATTCGTCAAGTGGGATGATGACTCCACTATTGTTACTCCAATTATTTTGAGGACTGATCC
  TCAGGGATTTTTCTTTTACTGGACAGACCAGAATAAGGAGACGGAGCTGTTAGATCTCAGCCTTGTCAAG
  GATGCCAGGTGTGGGAAGCACGCCAAAGCTCCCAAGGACCCCAAGTTGCGTGAACTTCTGGATGTTGGGA
  ACATCGGACACTTGGAACAGCGCATGATAACTGTGGTGTATGGACCAGACCTGGTGAACATTTCCCACTT
  GAATCTTGTGGCTTTTCAAGAGGAAGTAGCCAAGGAATGGACAAATGAGGTTTTCAGCTTGGCAACAAAC
  CTGCTGGCTCAGAACATGTCCAGGGACGCATTTCTGGAGAAAGCATATACTAAGCTCAAGCTCCAGGTCA
  CCCCAGAAGGCCGCATTCCTCTTAAAAACATCTATCGATTGTTCTCAGCAGACCGGAAGCGCGTTGAAAC
  TGCACTAGAGGCTTGTAGTCTTCCATCTTCGAGGAATGACTCCATTCCCCAAGAGGACTTCACTCCAGAT
  GTATACAGAGTTTTCCTGAACAATCTCTGTCCCCGACCTGAAATTGATAACATCTTTTCTGAATTTGGTG
  CCAAAAGCAAACCGTACCTGACGGTTGATCAGATGATGGATTTTATCAACCTTAAGCAGAGAGATCCCCG
  GCTCAATGAAATCCTTTACCCACCTCTGAAGCAGGAGCAGGTCCAAGTGTTGATTGAGAAATATGAGCCC
  AACAGCAGCCTCGCCAAGAAAGGGCAGATGTCAGTGGATGGATTCATGCGCTACCTGAGCGGAGAAGAAA
  ATGGAGTCGTTTCACCTGAGAAACTGGATTTGAACGAAGACATGTCTCAGCCCCTGTCTCACTATTTCAT
  CAATTCCTCGCACAACACCTACCTCACAGCTGGCCAGTTGGCTGGGAACTCGTCTGTAGAGATGTATCGC
  CAGGTGCTTCTGTCTGGATGTCGCTGTGTGGAGCTGGACTGCTGGAAGGGAAGGACCGCTGAGGAAGAGC
  CTGTCATTACCCATGGATTCACCATGACAACAGAAATATCCTTCAAGGAAGTCATAGAAGCCATCGCAGA
  GTGTGCGTTCAAGACGTCTCCTTTCCCCATCCTCCTTTCCTTTGAGAACCACGTGGATTCCCCGAAGCAA
  CAAGCCAAGATGGCCGAGTATTGCCGATTAATCTTTGGTGATGCCCTCCTGATGGAACCACTGGAAAAAT
  ACCCACTGGAATCTGGGGTACCTCTTCCAAGCCCTATGGATTTAATGTATAAAATCTTGGTGAAAAACAA
  GAAGAAGTCGCACAAGTCGTCAGAGGGAAGTGGTAAGAAGAAGCTTTCAGAGCAAGCTTCCAACACCTAC
  AGCGACTCCTCCAGTGTGTTCGAGCCTTCGTCTCCAGGAGCTGGGGAAGCAGATACGGAGAGTGATGACG
  ATGACGACGACGATGACTGTAAAAAGTCTTCCATGGATGAGGGGACAGCTGGCAGCGAAGCCATGGCCAC
  AGAAGAGATGTCTAACCTGGTGAACTATATTCAGCCTGTCAAGTTTGAGTCCTTTGAAATTTCAAAAAAA
  AGAAATAAAAGCTTTGAAATGTCTTCCTTCGTGGAAACCAAAGGACTTGAACAACTTACAAAGTCTCCGG
  TGGAATTTGTAGAATACAACAAGATGCAACTTAGCAGGATATATCCCAAGGGAACACGTGTGGATTCATC
  CAACTACATGCCTCAACTCTTCTGGAATGCTGGCTGTCAGATGGTGGCGCTCAACTTCCAGACAGTGGAT
  CTAGCTATGCAGATAAACATGGGCATGTATGAGTACAATGGGAAGAGCGGCTATAGGCTGAAGCCAGAGT
  TCATGAGGAGGCCGGACAAGCATTTTGATCCATTTACTGAAGGAATCGTAGATGGGATAGTGGCCAACAC
  TCTATCTGTTAAGATTATTTCAGGTCAGTTCCTCTCTGATAAGAAAGTTGGGACTTATGTGGAAGTGGAT
  ATGTTTGGCTTGCCTGTGGACACAAGGAGGAAGGCATTTAAAACCAAGACATCCCAAGGAAATGCTGTAA
  ACCCTGTCTGGGAAGAAGAACCTATTGTATTCAAAAAGGTAGTTCTACCTTCTCTGGCCTGTTTGAGGAT
  AGCAGCATATGAAGAGGGAGGCAAATTTATTGGCCACCGGATATTGCCTGTGCAGGCTATTCGGCCAGGC
  TATCACTACATCTGCCTGCGGAATGAGAGGAACCAGCCCCTGACGCTGCCAGCTGTCTTTGTCTACATAG
  AAGTCAAAGATTATGTGCCAGACACGTATGCAGATGTAATCGAAGCTCTATCAAACCCAATCCGATATGT
  CAATCTGATGGAACAGAGAGCTAAGCAGCTGGCTGCGTTGACCCTGGAGGATGAAGAGGAAGTAAAGAAG
  GAGGCTGACCCTGGAGAAACATCTTCCGAGGCTCCAAGCGAAACCAGGACAACGCCAGCAGAGAATGGGG
  TGAATCACACCGCATCCCTTGCACCCAAGCCGCCTTCCCAGGCTCCACACAGCCAGCCTGCTCCAGGGTC
  TGTGAAGGCACCTGCCAAAACAGAAGATCTGATCCAGAGCGTGTTAACAGAAGTAGAGGCGCAAACCATC
  GAAGAGCTCAAGCAACAGAAATCGTTCGTGAAACTTCAAAAGAAACACTACAAAGAAATGAAAGACCTGG
  TGAAGAGACACCACAAGAAAACCACGGAGCTCATTAAGGAGCACACGACCAAGTACAACGAGATTCAGAA
  TGACTACCTGAGAAGGAGGGCAGCCTTGGAGAAGTCCGCCAAAAAGGACAGCAAGAAGAAATCTGAACCC
  AGCAGCCCTGACCACGGCTCATCAGCCATTGAGCAAGACCTCGCTGCCCTGGATGCAGAAATGACTCAGA
  AGTTGATAGACTTGAAAGACAAGCAACAACAGCAGCTGCTTAATCTTCGGCAAGAGCAGTATTACAGCGA
  GAAGTACCAAAAGCGAGAACACATTAAACTGCTTATTCAGAAGTTGACAGATGTTGCAGAAGAGTGTCAG
  AACAATCAGTTAAAGAAGCTGAAGGAAATCTGCGAGAAAGAGAAGAAGGAATTAAAGAAGAAAATGGATA
  AGAAGAGGCAAGAGAAGATAACAGAAGCCAAGTCCAAAGACAAAAGCCAGATGGAAGAGGAGAAGACAGA
  GATGATCCGATCGTACATCCAGGAGGTGGTTCAGTACATCAAGAGGTTAGAGGAAGCACAAAGTAAAAGA
  CAAGAAAAACTCGTGGAGAAACACAATGAGATCCGCCAGCAGATCCTCGATGAGAAGCCCAAGGGGGAAG
  GCCCCTCCTCAGTCTTGTCGGAAGGTTGCCATGAGGATCCCTCTGTTCCCCCCAACTTTACTCCCCCCAA
  CCCTCAAGCTCTCAAGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226225 representing NM_019677
  Red=Cloning site Green=Tags(s)
  
  MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVK
  DARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATN
  LLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPD
  VYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEP
  NSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYR
  QVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQ
  QAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTY
  SDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKK
  RNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVD
  LAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVD
  MFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPG
  YHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKK
  EADPGETSSEAPSETRTTPAENGVNHTASLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTI
  EELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEP
  SSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQ
  NNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKR
  QEKLVEKHNEIRQQILDEKPKGEGPSSVLSEGCHEDPSVPPNFTPPNPQALKW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019677
• ORF Size: 3519 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019677.2, NP_062651.2
• RefSeq Size: 7116
• RefSeq ORF: 3522
• Locus ID: 18795