Musk (NM_001037130) Mouse Tagged ORF Clone

CAT#: MG226302

Musk (GFP-tagged) - Mouse muscle skeletal receptor tyrosine kinase (Musk) transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Musk
• Synonyms: Mdk4; Mlk; Nsk1; Nsk2; Nsk3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226302 representing NM_001037130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGAGCTTGTCAACATTCCACTGTTACAGATGCTCACCCTGGTTGCCTTCAGCGGGACTGAGAAAC
  TTCCAAAAGCCCCTGTCATCACCACGCCTCTTGAAACTGTAGATGCCTTGGTTGAAGAAGTAGCGACTTT
  CATGTGTGCCGTGGAATCCTACCCTCAGCCCGAGATTTCTTGGACCAGAAATAAAATTCTCATTAAGCTG
  TTTGACACCCGCTACAGCATCCGGGAGAATGGTCAGCTCCTCACCATTCTGAGCGTGGAAGACAGTGATG
  ATGGCATCTACTGCTGCATAGCCAACAATGGAGTGGGAGGAGCCGTGGAGAGTTGTGGTGCCCTGCAAGT
  GAAGATGAAACCTAAAATAACTCGTCCTCCCATTAATGTAAAAATAATAGAGGGATTGAAGGCAGTTCTG
  CCGTGCACTACGATGGGTAACCCCAAACCATCTGTGTCCTGGATCAAGGGGGACAATGCTCTCAGGGAAA
  ATTCCAGAATCGCAGTTCTTGAATCTGGGAGCTTAAGGATCCATAATGTGCAAAAGGAAGATGCAGGACA
  GTACCGCTGTGTGGCCAAAAACAGCCTGGGCACAGCTTACTCCAAACTGGTGAAGCTGGAAGTGGAGGTT
  TTTGCAAGAATCCTGCGTGCTCCTGAATCCCACAATGTCACCTTTGGTTCCTTTGTAACCCTACGCTGCA
  CAGCAATAGGCATCCCTGTCCCCACCATCAGCTGGATTGAAAACGGAAATGCTGTTTCTTCAGGTTCCAT
  TCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTCCAGCTCTTCATCACAAAGCCAGGACTCTAC
  ACATGCATAGCTACCAATAAGCACGGAGAAAAGTTCAGTACCGCAAAGGCTGCAGCCACTGTCAGCATAG
  CAGAATGGAGTAAGTCACAGAAAGACAGCCAAGGCTACTGTGCCCAGTACAGAGGGGAGGTGTGTGATGC
  AGTCCTGGCGAAAGATGCTCTTGTCTTCTTCAACACCTCCTACCGGGACCCCGAGGACGCCCAGGAGCTG
  CTGATCCACACTGCGTGGAATGAGCTGAAGGCTGTGAGTCCACTGTGCCGGCCAGCTGCTGAGGCTCTGC
  TGTGTAACCACCTCTTCCAAGAGTGCAGCCCTGGAGTGGTACCTACTCCCATGCCCATTTGCAGAGAGTA
  CTGCCTGGCGGTAAAGGAGCTCTTCTGTGCAAAGGAATGGCAGGCAATGGAAGGAAAGGCCCACCGGGGC
  CTCTACAGATCTGGGATGCATCTCCTTCCGGTACCAGAGTGCAGCAAGCTTCCCAGCATGCACCGGGACC
  CCACAGCCTGCACAAGACTGCCATATTTAGCATTCCCGTCAATAACGTCCTCCAGGCCGAGCGCGGACAT
  TCCAAACCTGCCTGCCTCCACCTCTTCCTTTGCCGTCTCGCCTGCGTACTCCATGACCGTCATCATCTCC
  ATCGTGTCCAGCTTTGCCCTGTTTGCTCTTCTCACCATCGCTACTCTCTATTGCTGCCGAAGGAGGAAAG
  AATGGAAAAATAAGAAAAGAGAGTCGACCGCGGTGACCCTCACCACGTTGCCTTCCGAGCTCCTGCTGGA
  TAGGCTCCATCCCAACCCCATGTACCAGAGGATGCCACTCCTTCTGAATCCTAAGTTGCTCAGCCTGGAG
  TATCCGAGGAATAACATTGAGTATGTCCGAGACATCGGAGAGGGGGCGTTTGGAAGAGTCTTCCAAGCAA
  GGGCCCCTGGCTTGCTGCCTTATGAACCTTTCACTATGGTGGCCGTGAAGATGCTTAAGGAAGAGGCCTC
  TGCAGACATGCAAGCGGACTTTCAGAGGGAGGCGGCCCTCATGGCAGAGTTTGACAACCCCAACATTGTG
  AAACTCTTAGGTGTGTGTGCCGTTGGGAAGCCGATGTGTCTGCTCTTTGAATATATGGCCTATGGTGACC
  TCAATGAGTTCCTCCGAAGTATGTCCCCGCACACTGTTTGCAGCCTCAGCCACAGTGACCTGTCCACGAG
  GGCTCGGGTGTCTAGCCCTGGTCCTCCACCACTGTCCTGTGCAGAACAGCTCTGCATTGCCAGGCAGGTG
  GCAGCTGGCATGGCCTACCTTTCAGAGCGCAAGTTTGTCCACCGGGACTTAGCTACCAGGAACTGCCTGG
  TTGGGGAGACCATGGTGGTGAAAATTGCAGACTTTGGCCTCTCCAGGAACATCTATTCCGCAGACTACTA
  CAAAGCTGATGGAAATGACGCCATCCCTATCCGCTGGATGCCGCCCGAGTCTATCTTCTACAACCGCTAC
  ACCACTGAGTCGGATGTATGGGCCTATGGTGTGGTCCTCTGGGAGATCTTCTCCTATGGGCTGCAGCCCT
  ACTATGGAATGGCCCACGAGGAGGTCATTTACTATGTGAGAGATGGCAACATCCTCGCCTGCCCTGAGAA
  CTGCCCCTTGGAACTGTACAACCTCATGCGCCTGTGTTGGAGCAAGCTGCCTGCTGATAGACCCAGCTTC
  TGCAGTATCCACAGGATCCTGCAGCGCATGTGCGAGAGAGCAGAGGGAACGGTGGGTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226302 representing NM_001037130
  Red=Cloning site Green=Tags(s)
  
  MRELVNIPLLQMLTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKL
  FDTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVL
  PCTTMGNPKPSVSWIKGDNALRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEV
  FARILRAPESHNVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRLQLFITKPGLY
  TCIATNKHGEKFSTAKAAATVSIAEWSKSQKDSQGYCAQYRGEVCDAVLAKDALVFFNTSYRDPEDAQEL
  LIHTAWNELKAVSPLCRPAAEALLCNHLFQECSPGVVPTPMPICREYCLAVKELFCAKEWQAMEGKAHRG
  LYRSGMHLLPVPECSKLPSMHRDPTACTRLPYLAFPSITSSRPSADIPNLPASTSSFAVSPAYSMTVIIS
  IVSSFALFALLTIATLYCCRRRKEWKNKKRESTAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLE
  YPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMAEFDNPNIV
  KLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSTRARVSSPGPPPLSCAEQLCIARQV
  AAGMAYLSERKFVHRDLATRNCLVGETMVVKIADFGLSRNIYSADYYKADGNDAIPIRWMPPESIFYNRY
  TTESDVWAYGVVLWEIFSYGLQPYYGMAHEEVIYYVRDGNILACPENCPLELYNLMRLCWSKLPADRPSF
  CSIHRILQRMCERAEGTVGV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037130
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037130.1, NP_001032207.1
• RefSeq Size: 3330 bp
• RefSeq ORF: 2583 bp
• Locus ID: 18198
• Cytogenetics: 4 31.87 cM
• Gene Summary: This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]