Cd2ap (NM_009847) Mouse Tagged ORF Clone

CAT#: MG226345

Cd2ap (GFP-tagged) - Mouse CD2-associated protein (Cd2ap), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd2ap
• Synonyms: AL024079; C78928; METS-1; Mets1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226345 representing NM_009847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGACTATATTGTGGAGTATGACTATGATGCTGTACATGATGATGAGTTAACTATCCGAGTTGGGG
  AAATCATCAGAAATGTGAAAAAACTACAGGAAGAAGGATGGCTAGAAGGAGAACTAAATGGGAGACGAGG
  GATGTTCCCTGACAATTTTGTTAAGGAAATTAAGCGAGAGACAGAACCCAAGGATGACAATTTGCCCATC
  AAACGGGAAAGGCAAGGAAATGTAGCAAGTCTTGTACAGCGAATCAGCACTTATGGACTTCCAGCTGGAG
  GAATTCAGCCACATCCACAAACCAAAGCTATTAAGAAGAAGACAAAAAAGCGTCAGTGTAAAGTTCTTTT
  TGACTACTCTCCACAAAATGAGGACGAACTGGAATTGATCGTGGGGGATGTCATTGACGTCATTGAAGAG
  GTAGAAGAAGGCTGGTGGAGTGGAACCCTGAACAATAAGTTGGGACTGTTTCCCTCAAACTTTGTGAAAG
  AATTAGAGTCCACAGAGGATGGTGAAACTCACAACGCTCAGGAGGAATCAGAAGTTCCTTTGACTGGCCC
  TACCTCACCGTTGCCATCTCCGGGGAATGGGAGTGAACCTGCTCCCGGATCAGTTGCACAGCCAAAGAAA
  ATCCGAGGAATTGGATTTGGAGACATCTTTAAAGAAGGCTCTGTGAAACTTCGGACAAGAACTTCCAGTA
  GTGAAACAGAGGAGAAAAAAACAGAAAAGCCTTTAATCCTACAGCCTCTGGGATCTAGAACTCAGAATGT
  GGAGGTAACAAAACCAGACGTTGATGGCAAGATTAAAGCTAAGGAATATTGTAGAACATTATTTCCCTAT
  ACAGGAACCAATGAAGATGAACTTACATTTAGAGAAGGGGAGATTATCCATTTGATAAGTAAGGAGACTG
  GAGAAGCAGGCTGGTGGAAGGGTGAACTGAACGGTAAAGAAGGAGTGTTTCCAGATAACTTTGCTGTTCA
  GATAAGTGAACTAGATAAAGACTTTCCAAAACCAAAGAAACCACCTCCTCCTGCAAAAGGTCCAGCTCCA
  AAGCCTGACCTGTCAGCTGCAGAGAAGAAAGCTTTCCCTCTAAAGGCTGAAGAAAAAGATGAAAAATCAC
  TGTTAGAACAGAAACCTTCTAAACCAGCTGCTCCTCAAGTCCCGCCCAAAAAGCCCACTGCACCCACCAA
  AGCCAGTAACTTACTGAGGTCGCCTGGAGCAGTGTACCCAAAGCGGCCGGAAAAGCCAGTCCCTCCGCCA
  CCTCCAGCAGCCAAAATTAATGGAGAAGTTTCTATAATTTCTTCAAAGATTGATACTGAGCCAGTATCAA
  AACCAAAGCTAGATCCTGAACAATTGCCAGTTAGACCAAAATCAGTAGACCTTGATGCCTTTGTAGCCAG
  GAACTCAAAAGAAACAGATGATGTAAATTTTGATGACATAGCTTCCTCAGAGAACTTGTTACATCTCACT
  GCTAATAGACCCAAGATGCCTGGAAGACGATTGCCTGGCCGCTTCAACGGTGGACATTCTCCAACCCAAA
  GCCCAGAAAAAACCTTGAAGCTACCAAAAGAAGATGATAGTGGCAACCTAAAGCCTTTGGAGTTTAAAAA
  GGATGCAAGCTACTCTTCAAAGCCATCTCTTTCAACACCTTCAAGTGCTTCTAAAGTAAATACAGCTGCT
  TTCCTAACTCCGTTAGAACTCAAAGCTAAAGCAGAAGCAGATGATGGGAAAAAAAATTCCGTGGACGAAC
  TTAGAGCCCAGATTATTGAATTGTTGTGTATTGTAGATGCACTGAAAAAGGATCATGGGAAAGAACTGGA
  AAAGCTACGCAAAGAATTAGAAGAAGAGAAGGCCATGCGAAGTAATCTAGAGGTGGAAATAGCAAAGCTG
  AAGAAAGCTGTTCTGTTGTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226345 representing NM_009847
  Red=Cloning site Green=Tags(s)
  
  MVDYIVEYDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEIKRETEPKDDNLPI
  KRERQGNVASLVQRISTYGLPAGGIQPHPQTKAIKKKTKKRQCKVLFDYSPQNEDELELIVGDVIDVIEE
  VEEGWWSGTLNNKLGLFPSNFVKELESTEDGETHNAQEESEVPLTGPTSPLPSPGNGSEPAPGSVAQPKK
  IRGIGFGDIFKEGSVKLRTRTSSSETEEKKTEKPLILQPLGSRTQNVEVTKPDVDGKIKAKEYCRTLFPY
  TGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQISELDKDFPKPKKPPPPAKGPAP
  KPDLSAAEKKAFPLKAEEKDEKSLLEQKPSKPAAPQVPPKKPTAPTKASNLLRSPGAVYPKRPEKPVPPP
  PPAAKINGEVSIISSKIDTEPVSKPKLDPEQLPVRPKSVDLDAFVARNSKETDDVNFDDIASSENLLHLT
  ANRPKMPGRRLPGRFNGGHSPTQSPEKTLKLPKEDDSGNLKPLEFKKDASYSSKPSLSTPSSASKVNTAA
  FLTPLELKAKAEADDGKKNSVDELRAQIIELLCIVDALKKDHGKELEKLRKELEEEKAMRSNLEVEIAKL
  KKAVLLS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009847
• ORF Size: 1911 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009847.3, NP_033977.3
• RefSeq Size: 5427
• RefSeq ORF: 1914
• Locus ID: 12488
• Gene Summary: This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. The mouse genome contains at least two pseudogenes located on chromosomes 9 and 17. [provided by RefSeq, Jul 2008]