Dclk1 (NM_001195538) Mouse Tagged ORF Clone

CAT#: MG226414

Dclk1 (GFP-tagged) - Mouse doublecortin-like kinase 1 (Dclk1) transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dclk1
• Synonyms: 1700113D08Rik; 2810480F11Rik; AI836758; Click-I; Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226414 representing NM_001195538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTTCGGCAGAGATATGGAGTTGGAGCATTTTGATGAGCGGGACAAGGCGCAGAGGTACAGCAGGG
  GGTCCCGTGTGAATGGCCTGCCCAGCCCCACACACAGCGCCCACTGCAGCTTCTACCGCACCCGCACCCT
  GCAGACGCTCAGCTCCGAGAAGAAAGCCAAGAAGGTTCGATTCTACAGAAATGGTGACCGCTACTTCAAA
  GGAATTGTGTATGCCATCTCCCCAGACCGCTTCAGATCTTTCGAGGCCCTGCTGGCTGATTTGACCCGAA
  CTCTCTCGGATAATGTGAATTTGCCCCAGGGGGTGAGAACCATCTACACCATCGATGGACTCAAGAAGAT
  CTCCAGCCTGGACCAGCTGGTGGAAGGTGAAAGCTATGTCTGCGGCTCCATCGAGCCCTTTAAGAAGCTG
  GAGTACACCAAGAATGTGAACCCCAACTGGTCAGTGAACGTCAAGACCACCTCAGCCTCCCGCGCAGTGT
  CTTCTTTGGCCACTGCCAAGGGTGGGCCTTCGGAGGTTCGGGAGAATAAGGATTTCATTCGACCCAAGCT
  GGTCACCATCATCAGAAGTGGGGTGAAGCCACGGAAGGCTGTCAGAATCCTGCTGAACAAGAAGACGGCT
  CACTCCTTCGAGCAGGTTCTCACTGACATTACCGACGCTATCAAGCTGGACTCCGGTGTGGTGAAGCGCC
  TGTACACTCTGGATGGGAAGCAGGTGATGTGCCTTCAGGACTTTTTTGGTGACGATGACATTTTTATTGC
  ATGTGGACCAGAGAAGTTCCGTTACCAGGATGATTTCTTGCTAGATGAAAGTGAATGTCGAGTGGTGAAA
  TCAACTTCTTACACCAAAATAGCATCAGCGTCCCGCAGAGGCACAACCAAGAGCCCAGGACCTTCCCGGA
  GAAGCAAGTCCCCAGCCTCCACCAGCTCAGTTAATGGAACCCCTGGTAGTCAGCTCTCTACTCCACGCTC
  GGGCAAGTCACCAAGTCCATCACCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGC
  GGCTCCTCGACTTCACTTTCATCCACTAAAGTTTGCAGCTCAATGGATGAGAATGATGGCCCTGGGGAAG
  AAGAGTCTGAGGAAGGCTTCCAGATTCCTGCCACAATAACAGAGAGATACAAAGTCGGGAGAACAATAGG
  AGACGGAAATTTTGCTGTTGTCAAGGAATGTATAGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATC
  ATCAAGAAAAGCAAATGCCGAGGCAAAGAGCACATGATCCAGAACGAGGTCTCCATCCTACGGAGGGTGA
  AGCACCCCAACATTGTCCTCCTGATTGAAGAGATGGATGTGCCGACTGAACTGTATCTTGTAATGGAATT
  AGTGAAGGGTGGAGACCTTTTCGATGCCATCACCTCCACTAGCAAATACACAGAGAGAGATGCCAGCGGG
  ATGCTGTACAACCTGGCCAGCGCCATCAAATACCTGCACAGCCTGAACATCGTCCACCGTGACATCAAGC
  CAGAGAATCTGCTGGTGTATGAGCACCAGGATGGCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGC
  CACAATTGTCGACGGCCCCCTGTACACAGTCTGTGGCACCCCAACATATGTGGCTCCAGAAATCATTGCA
  GAGACTGGATATGGCCTCAAGGTGGACATCTGGGCAGCTGGCGTGATCACTTATATCCTGCTGTGTGGCT
  TCCCTCCGTTCCGTGGAAGTGGGGATGACCAGGAGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGA
  CTTTCCATCTCCGTATTGGGACAATGTGTCAGATTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTT
  AACGTGGACCAGAGATTTTCAGCCGTGCAGGTCCTTGAGCATCCCTGGGTTAATGATGATGGTCTCCCAG
  AAAATGAGCATCAGCTGTCAGTAGCTGGCAAAATCAAGAAGCATTTCAACACAGGCCCCAAGCCGAGCAG
  CACTGCAGCAGGAGTTTCTGTAATAGCAACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGA
  CGCAACCAGGATGTGAGGAGCCGGTACAAGGCGCAGCCAGCTCCACCGGAATTGAACTCGGAATCGGAGG
  ACTACTCCCCCAGCTCCTCTGAGACTGTTCGCTCCCCCAATTCGCCCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226414 representing NM_001195538
  Red=Cloning site Green=Tags(s)
  
  MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRTRTLQTLSSEKKAKKVRFYRNGDRYFK
  GIVYAISPDRFRSFEALLADLTRTLSDNVNLPQGVRTIYTIDGLKKISSLDQLVEGESYVCGSIEPFKKL
  EYTKNVNPNWSVNVKTTSASRAVSSLATAKGGPSEVRENKDFIRPKLVTIIRSGVKPRKAVRILLNKKTA
  HSFEQVLTDITDAIKLDSGVVKRLYTLDGKQVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESECRVVK
  STSYTKIASASRRGTTKSPGPSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRISQHG
  GSSTSLSSTKVCSSMDENDGPGEEESEEGFQIPATITERYKVGRTIGDGNFAVVKECIERSTAREYALKI
  IKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASG
  MLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIA
  ETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLV
  NVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPSSTAAGVSVIATTALDKERQVFRRR
  RNQDVRSRYKAQPAPPELNSESEDYSPSSSETVRSPNSPF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195538
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001195538.1, NP_001182467.1
• RefSeq Size: 7817
• RefSeq ORF: 2223
• Locus ID: 13175
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the biological validity of some variants has not been determined. These variants encode different isoforms, which are differentially expressed and have different kinase activities. [provided by RefSeq, Sep 2010]