Usp33 (NM_133247) Mouse Tagged ORF Clone

CAT#: MG226445

Usp33 (GFP-tagged) - Mouse ubiquitin specific peptidase 33 (Usp33) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Usp33
• Synonyms: 9830169D19Rik; AA409780; Vdu1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226445 representing NM_133247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATAACGAAAGAGGACTTGATAC
  AGAAATCTCTCGGCGCTTGTCAGGACTGTAAAGTCAGAGGACCAAACCTGTGGGCGTGCCTGGAGAATAG
  GTGCTCCTATGTTGGCTGCGGGGAGTCGCAAGTGGACCACAGCACCATACACTCGCAGGAGACAAAGCAT
  TATCTAACTGTGAACCTCACCACTCTTCGAGTATGGTGTTATGCTTGCAGCAAAGAAGTATTTTTGGATA
  GAAAATTAGGAACTCCTCCTTCATTACCTCATGTAAGACAGCCTCAACAAACACAAGAAAACAGTGTCCA
  GGATTTTAAAATTCCCAGTAATCCAGCATTGAAAACCCCCATGGTTGCTGTGTCTGAAGATCTGGATATA
  GAAGTGGAAGAGGAAGACGAGCTGAAGGCTAGAGGCCTGACAGGTTTGAAAAACATTGGAAATACTTGTT
  ATATGAACGCGGCGCTGCAGGCTCTTTCTAACTGCCCACCTTTGACACAGTTCTTTCTTGATTGTGGAGG
  ACTGGCTAGAACAGATAAGAAACCAGCAATTTGTAAAAGTTATCTCAAACTAATGACCGAGTTGTGGCAC
  AAGAGCAGGCCAGGATCTGTTGTGCCGGCTAATCTGTTCCAAGGAATTAAAACTGTAAATCCAACTTTTC
  GAGGTTATTCTCAGCAGGATGCCCAGGAATTCCTTCGCTGTCTAATGGACCTGCTTCATGAGGAGCTGAA
  GGAGCAGGTCATGGAAATGGAGGAAGAGCCTCAAACACTAACTTCTGAGGAGACGGTGGAGGAAGAGAAG
  AGCCAGTCAGATGTGGATTTTCAGTCGTGCGAGTCTTGTAGCAGCAGCGAGAAAGCAGAAAATGAGAGTG
  GCTCCAAAGGCTTTCCTGAGGACAGCAATGAGACCACCATGCTCATCCAGGACGAGGATGACCTGGAGAT
  GGCCAAAGACTGGCAGAAAGAGAAGGTGTGCAATAAGATCAACAAGGCAAATGCCGATGTAGAACTGGAC
  AAAGACAGGGACACAGTGTGTGAAACAGTTGACCTAAACAGCCAGGAGACCGTCAAAGTGCAGATACACG
  GCAGAGCCTCAGAATCTATCACTGATGTCCATCTGAATGACCTAGCTACGTCACAGATCCTTCCTTCAAA
  TGAAAGTGTTAGTCCACGGTTATCAGCAAGCCCTCCTAAGCTAGGCAGTCTGTGGCCAGGACTGTCGCCT
  CCACACAAGAAAGCTCAGTCTACATCTGCAAAGAGGAAAAAGCAGCATAAGAAATACAGAAGTGTCATCT
  CCGACATATTCGATGGAACAGTCATTAGCTCGGTACAGTGTCTGACGTGCGATAGGGTGTCTATAACCCT
  CGAGACCTTTCAGGATCTGTCCTTGCCGATTCCTGGCAAGGAGGACCTGGCTAAGCTGCACTCCTCCAGT
  CACCCAACTATAGTCAAAGCAGGGTCATGTGGTGAAGCGTACGCCCCGCAGGGGTGGATAGCTTTCTTCA
  TGGAGTATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCCAGTAGTTACCTTGCA
  AGATTGTCTTGCTGCCTTCTTCGCCAGAGATGAACTTAAAGGTGACAACATGTACAGTTGTGAAAAATGC
  AAAAAGTTGAGGAATGGAGTAAAGTTTTGTAAAGTACAGAAGTTTCCTGAGATTTTGTGTATCCACCTTA
  AAAGATTTCGACATGAACTGATGTTTTCCACCAAAATTAGCACCCATGTTTCCTTCCCCCTGGAAGGCCT
  CGATCTTCAGCCATTTCTTGCAAAGGACAGCCCAGCTCAGATTGTGACATATGATCTCCTGTCAGTCATC
  TGTCACCATGGGACTGCAAGTAGTGGGCACTACATTGCCTACTGCCGAAACAATTTAAATAACCTGTGGT
  ATGAGTTTGATGACCAGAGCGTCACTGAAGTTTCAGAGTCCACTGTACAGAATGCCGAGGCCTACGTCCT
  TTTCTACAGGAAGAGCAGTGAAGAGGCACAAAAGGAGAGGCGGAGGATATCAAATTTGTTGAACATCATG
  GAACCTAGCCTCCTTCAGTTCTACATATCTCGACAGTGGTTGAATAAATTTAAGACCTTTGCTGAACCTG
  GCCCTATTTCAAATAATGATTTTCTCTGTATCCATGGAGGTATTCCTCCACGAAAAGCGAGTTATATTGA
  AGACTTAGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTCTATAGCAGGTATGGAGGAGGGCCTGCT
  GTCAACCATCTCTACATCTGCCACACCTGCCAAATTGAGTTAGAGAAGATTGAAAAACGAAGAAAAACCG
  AATTGGAAATTTTTATTCGGCTCAACAGAGCATTTCAAGAGGAGGACTCCCCAGCTACTTTTTACTGTAT
  CAGCATGCAGTGGTTTAGAGAATGGGAGAGTTTTGTAAAGGGTAAGGATGGAGATCCCCCAGGTCCAATC
  GACAACACTAAAATTGCGGTTACTAAATGTGGCAGTGTGATGCTCAAGCAAGGAGCAGACTCTGGTCAAA
  TTTCAGAAGAAACATGGAATTTCCTGCAGTCTATATATGGTGGGGGGCCTGAAGTTATCCTCCGACCTCC
  AGTTGTTCATGTTGACCCTGATGTACTCCAAGCAGAGGAAAAGATTGAAGTAGAAACTCGCTCTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226445 representing NM_133247
  Red=Cloning site Green=Tags(s)
  
  MTTFRNHCPHLDSVGEITKEDLIQKSLGACQDCKVRGPNLWACLENRCSYVGCGESQVDHSTIHSQETKH
  YLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNPALKTPMVAVSEDLDI
  EVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWH
  KSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEMEEEPQTLTSEETVEEEK
  SQSDVDFQSCESCSSSEKAENESGSKGFPEDSNETTMLIQDEDDLEMAKDWQKEKVCNKINKANADVELD
  KDRDTVCETVDLNSQETVKVQIHGRASESITDVHLNDLATSQILPSNESVSPRLSASPPKLGSLWPGLSP
  PHKKAQSTSAKRKKQHKKYRSVISDIFDGTVISSVQCLTCDRVSITLETFQDLSLPIPGKEDLAKLHSSS
  HPTIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKC
  KKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVI
  CHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIM
  EPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPA
  VNHLYICHTCQIELEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPI
  DNTKIAVTKCGSVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDVLQAEEKIEVETRSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133247
• ORF Size: 2727 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133247.3, NP_573510.2
• RefSeq Size: 4206
• RefSeq ORF: 2730
• Locus ID: 170822