Ddx27 (NM_153065) Mouse Tagged ORF Clone

CAT#: MG226500

Ddx27 (GFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ddx27
• Synonyms: C86129
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226500 representing NM_153065
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCGGAGCTCGGCTTCATCCGGACCATCGGCGAGAATGACGAGGTGCCGGTTGAGCCCGAGTCGG
  ACTCCGGAGACGAGGAGGAGGAGGGACCCATTGTGCTGGGCCGCAAGCAGAAAGCCTTGCAGAAGAACCG
  CAGTGCTGACTTCAACCCTGACTTTGTTTTCACTGAGAAGGAGGGCATGTATGATGGCAGCTGGGCCCTG
  GCTGATGTCATGAGCCAGCTCAAGAAGAAGCGGGCAGCCACTACACTAGATGAGAAGATTGAGAAAGTCC
  GGAAGAGAAGGAAGGCAGAGGACAAGGAAGCCAAGTCTGGGAAGGTGGAGGAGAAAGAAGGCCAGGCAGA
  CTCTGACCTGAAGGGGCAGGAGAATCCGGGTGAGGATGAGGCAGGCTCCAAGGATGAAGACTCTGAGACT
  GACTACTCCTCTGAGGATGAGGAAATCCTCACCAAAGCAGACACACTCAAAGTGAAGGAGAAGAAGAAGA
  AGAAGAAGGGCCAGGCAGCAGGAGGATTCTTTGAAGATGCATCCGAGTATGACAAAAGCCTGTCCTTCCA
  GGACATGAACCTCTCCCGGCCCCTCCTGAAGGCCATTACAGCCATGGGCTTCAAGCAGCCCACGCCAATC
  CAGAAGGCGTGTATCCCTGTGGGTCTGCTGGGGAAGGACATCTGCGCCTGTGCAGCCACTGGGACAGGCA
  AAACTGCAGCCTTTGCACTGCCCGTCTTGGAGCGTCTGATCTACAAGCCCCGCCAGGCTGCAGTCACTCG
  CGTGCTGGTGCTGGTCCCCACGAGAGAGCTGGGCATCCAAGTGCACTCTGTCACCAAGCAGCTGGCCCAG
  TTCTGCAGCATCACTACCTGCCTGGCTGTGGGTGGCCTGGATGTGAAGTCTCAGGAAGCCGCTCTTCGGG
  CAGCACCAGACATCCTCATTGCCACACCTGGCCGGCTCATCGACCACCTCCATAACTGCCCCTCCTTCCA
  CCTGAGCAGCATTGAAGTGCTCATCTTGGATGAGGCTGACAGGATGCTGGATGAGTATTTCGAGGAGCAG
  ATGAAGGAGATTATCCGAATGTGTTCCCACCACCGCCAGACCATGCTCTTCTCGGCCACCATGACAGATG
  AGGTGAAAGATCTGGCTTCGGTCTCCTTAAAGAATCCTGTGCGGATATTTGTGAACAGCAACACAGATGT
  CGCTCCCTTCCTGCGGCAAGAGTTCATACGGATCCGGCCTAATAGGGAAGGGGACCGGGAGGCCATTGTG
  GCAGCTCTGCTGATGAGAACCTTCACTGACCACGTGATGCTCTTCACCCAGACCAAGAAGCAGGCCCATC
  GCATGCACATCCTCCTGGGGCTGCTGGGGCTGCAGGTGGGCGAGCTCCACGGCAACTTGTCACAGACCCA
  GCGCCTGGAGGCCCTCCGGCGCTTTAAGGACGAACAGATTGACATCCTGGTGGCCACAGATGTGGCAGCC
  CGCGGTCTTGACATTGAAGGAGTGAAAACAGTGATCAACTTCACCATGCCCAACACTGTTAAACACTATG
  TGCACCGGGTGGGGCGGACGGCCCGTGCTGGCAGGGCTGGACGCTCTGTCTCCCTAGTGGGAGAGGAGGA
  GCGGAAGATGCTGAAGGAGATCGTGAAGGCTGCCAAGGCGCCAGTGAAGGCTCGGATCCTTCCCCAGGAT
  GTTATCCTCAAGTTCCGGGACAAGATTGAGAAATTGGAGAAAGATGTGTATGCAGTGCTGCAGCTGGAGG
  CGGAGGAAAAAGAGATGCAGCAGTCGGAAGCCCAGATTGACACAGCACAGCGACTCTTAGCCAAAGGGAA
  AGAGACAGCAGACCAGGAGCCTGAGAGGAGCTGGTTCCAGACCAAAGAAGAGAGGAAGAAGGAGAAGATT
  GCTAAGGCTCTACAGGAGTTTGACTTGGCCTTAAGAGGAAAGAAGAAAAGGAAGAAGTTTATGAAGGATG
  CCAAGAAAAAGGGGGAGATGACGGCAGAGGAGAGGTCCCAGTTTGAAATCCTCAAGGCACAGATGTTTGC
  AGAGCGGCTGGCCAAGAGGAATCGCAGAACCAAGCGAGCACGGGCAATGCCTGAGGACGAGCCTACAGGC
  CCTGCTAAAAAGCAGAAGCAGCAGCAGAAGTCTGTGTTTGATGAAGAACTCACCAACACAAGCAAGAAGG
  CCCTGAAACAGTATCGGGCTGGCCCTTCCTTTGAAGAGAGGAAACAGTCAGGTCTGCCCCGCCAGAGGAG
  AGGGAACTTCAAATCTAAGTCCAGGTACAAGAGAAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226500 representing NM_153065
  Red=Cloning site Green=Tags(s)
  
  MLAELGFIRTIGENDEVPVEPESDSGDEEEEGPIVLGRKQKALQKNRSADFNPDFVFTEKEGMYDGSWAL
  ADVMSQLKKKRAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGEDEAGSKDEDSET
  DYSSEDEEILTKADTLKVKEKKKKKKGQAAGGFFEDASEYDKSLSFQDMNLSRPLLKAITAMGFKQPTPI
  QKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQ
  FCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQ
  MKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIV
  AALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAA
  RGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQD
  VILKFRDKIEKLEKDVYAVLQLEAEEKEMQQSEAQIDTAQRLLAKGKETADQEPERSWFQTKEERKKEKI
  AKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRTKRARAMPEDEPTG
  PAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQSGLPRQRRGNFKSKSRYKRKK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153065
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153065.3, NP_694705.2
• RefSeq Size: 2592
• RefSeq ORF: 2283
• Locus ID: 228889