Apaf1 (NM_001042558) Mouse Tagged ORF Clone

CAT#: MG226529

Apaf1 (GFP-tagged) - Mouse apoptotic peptidase activating factor 1 (Apaf1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Apaf1
• Synonyms: 6230400I06Rik; Apaf-1; Apaf1l; fog; mKIAA0413
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226529 representing NM_001042558
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCAAAGGCCCGCAATTGTTTGCTTCAACATAGAGAAGCTTTGGAAAAGGACATCAAAACATCCT
  ACATCATGGATCACATGATCAGTAATGGCGTCTTGTCAGTGATAGAGGAGGAGAAGGTCAAAAGTCAGGC
  CACTCAATATCAACGAGCAGCCGCTTTAATTAAAATGATACTTAATAAAGACAACTGTGCCTACATTTCA
  TTCTACAACGCTCTGCTACACGAGGGCTATAAGGACCTTGCTGCGCTTCTGCAGAGTGGCCTTCCTCTTG
  TGTCGTCTTCCAGTGGCAAGGACACAGATGGTGGAATAACTTCATTTGTAAGGACAGTGCTGTGTGAAGG
  TGGAGTACCCCAGAGGCCGGTTATTTTCGTTACTAGAAAGAAGCTGGTTCATGCGATTCAGCAGAAGCTC
  TGGAAACTGAATGGAGAACCAGGGTGGGTCACCATCTATGGGATGGCAGGCTGCGGCAAGTCTGTGTTAG
  CTGCGGAAGCCGTTCGAGATCACTCCCTCTTAGAAGGTTGCTTTTCAGGGGGTGTACACTGGGTTTCCAT
  TGGAAAACAAGACAAATCTGGGCTTCTCATGAAACTGCAGAATCTGTGCATGCGCTTGGACCAAGAAGAG
  AGTTTCTCTCAGAGGCTTCCACTTAATATTGAGGAGGCCAAAGACCGCCTCCGTGTTCTGATGCTGCGCA
  AACACCCAAGGTCTCTGTTGATCTTGGATGATGTTTGGGATCCTTGGGTGTTAAAAGCTTTTGACAATCA
  GTGTCAGATTCTTCTTACAACCAGAGATAAGAGTGTTACAGATTCAGTAATGGGTCCTAAGCATGTTGTC
  CCTGTGGAGAGTGGTCTAGGGAGAGAGAAAGGACTTGAGATCTTGTCACTTTTTGTTAACATGAAGAAAG
  AAGATCTGCCAGCGGAGGCTCACAGTATTATAAAGGAATGCAAAGGTTCTCCTCTTGTAGTGTCTTTAAT
  TGGTGCACTTTTACGTGATTTTCCCAATCGCTGGGCATACTACCTCAGACAGCTTCAGAATAAGCAGTTT
  AAGAGAATAAGGAAGTCTTCATCTTATGATTATGAGGCTCTAGATGAAGCCATGTCGATAAGTGTTGAAA
  TGCTCAGAGAAGACATCAAAGACTATTACACAGACCTTTCCATCCTTCAGAAGGACGTCAAGGTACCTAC
  AAAGGTGTTGTGCGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCCTGCAGGAGTTCGTTAAT
  AAGTCTCTCTTATTCTGTAATCGGAATGGAAAGTCATTTTGTTATTATTTACACGATCTTCAAGTAGATT
  TTCTTACAGAGAAGAATCGCAGTCAGCTTCAGGATCTGCACAGGAAGATGGTCACTCAGTTTCAGAGGTA
  TTACCAGCCCCACACGCTGTCTCCAGACCAGGAGGACTGCATGTATTGGTACAACTTCCTAGCCTATCAC
  ATGGCTAGTGCCAATATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGACTGGATTAAAGCAAAAA
  CAGAACTTGTCGGCCCTGCCCATCTGATTCACGAGTTCGTGGCATATAGGCATATATTGGATGAAAAGGA
  TTGTGCAGTCTGTGAGAATTTTCAAGAGTTTTTATCTTTAAATGGACACCTCCTTGGACGACAGCCATTT
  CCTAATATTGTACAGCTGGGCCTCTGTGAACCAGAAACTTCCGAAGTTTATCGACAAGCAAAGCTGCAGG
  CCAAGCAGGAGGGGGATACTGGGCGCCTTTACCTGGAATGGATAAACAAAAAAACTATCAAGAATCTGTC
  CCGCTTAGTCGTCCGCCCCCACACAGATGCTGTTTACCACGCGTGTTTTTCTCAGGATGGCCAGAGAATA
  GCTTCTTGTGGGGCTGATAAAACCTTACAGGTGTTCAAAGCCGAGACAGGAGAGAAACTTCTTGACATTA
  AAGCTCATGAAGATGAGGTGCTCTGCTGCGCGTTCTCCTCAGACGACAGTTACATAGCGACCTGCTCAGC
  GGATAAGAAGGTTAAGATTTGGGATTCTGCGACTGGGAAGCTTGTGCACACCTACGACGAGCACTCGGAG
  CAAGTCAATTGCTGCCATTTCACCAACAAGAGTAACCACCTTCTCTTGGCCACCGGGTCAAATGATTTCT
  TCCTCAAGCTCTGGGATTTGAATCAAAAAGAATGTCGAAATACCATGTTTGGTCACACGAACTCAGTCAA
  CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCTAGCTGCTCAGCTGACGGGACTTTAAGGCTTTGG
  GATGTGAGATCAGCAAACGAGAGGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCTC
  CAGAGGATGTGGAGGTGATCGTGAAGTGTTGTTCCTGGTCTGCAGATGGTGACAAAATAATAGTGGCAGC
  AAAAAACAAAGTCCTCCTTTTTGATATTCATACTAGTGGCCTATTGGCAGAGATCCACACAGGCCATCAC
  AGCACCATCCAGTACTGTGACTTCTCCCCCTATGACCATTTGGCTGTGATTGCCCTGTCTCAGTACTGTG
  TGGAGTTGTGGAACATAGACTCCCGCCTAAAGGTGGCCGACTGCAGAGGACATTTGAGTTGGGTTCACGG
  TGTGATGTTTTCTCCCGATGGCTCCTCATTTTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG
  ACAAAAAAGGTATGCAAGAACTCTGCCATCGTGCTAAAGCAGGAAATAGACGTCGTGTTTCAAGAGAACG
  AAACGATGGTCCTTGCAGTTGACAACATAAGAGGCCTGCAACTCATTGCTGGAAAAACAGGCCAGATTGA
  TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTCAGTCCACACCTTGAGTACGTGGCATTCGGAGAT
  GAAGATGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTCTTCAGTTCTGGGGTTGGGCACAAGA
  AAGCTGTGCGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGATTCTGTGAT
  TCAGGTATGGAATTGGCAGACAGGGGACTATGTATTTTTGCAAGCCCACCAGGAAACGGTAAAGGACTTC
  AGGCTCCTCCAAGATTCAAGATTGCTTTCTTGGTCATTTGATGGAACGGTGAAGGTGTGGAATGTCATTA
  CCGGAAGAATAGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC
  GACCAAGTTTTCCTCTACCTCTGCTGATAAGACTGCCAAGATCTGGAGTTTTGACCTCCTTTCCCCTCTT
  CATGAGCTGAAGGGCCATAATGGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTGGCAA
  CTGGAGATGACAATGGAGAAATCCGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTCGTGTGCTCC
  GATCTCGGTAGAGGAAGGAACTGCTACCCACGGCGGCTGGGTAACTGATGTGTGCTTCTCTCCCGACAGT
  AAAACGCTTGTCTCTGCTGGAGGATATCTCAAGTGGTGGAATGTTGCCACTGGGGACTCCTCACAGACCT
  TCTACACAAATGGAACAAACCTCAAGAAAATCCACGTGTCCCCTGACTTCAGAACCTATGTGACTGTCGA
  TAATCTCGGTATTTTATATATTTTACAGGTTTTAGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226529 representing NM_001042558
  Red=Cloning site Green=Tags(s)
  
  MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYIS
  FYNALLHEGYKDLAALLQSGLPLVSSSSGKDTDGGITSFVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKL
  WKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEE
  SFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVV
  PVESGLGREKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQF
  KRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVN
  KSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYH
  MASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPF
  PNIVQLGLCEPETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRI
  ASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSE
  QVNCCHFTNKSNHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLW
  DVRSANERKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHH
  STIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWE
  TKKVCKNSAIVLKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGD
  EDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDF
  RLLQDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPL
  HELKGHNGCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDS
  KTLVSAGGYLKWWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042558
• ORF Size: 3747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042558.1, NP_001036023.1
• RefSeq Size: 6557
• RefSeq ORF: 3750
• Locus ID: 11783