Gli3 (NM_008130) Mouse Tagged ORF Clone

CAT#: MG226591

Gli3 (GFP-tagged) - Mouse GLI-Kruppel family member GLI3 (Gli3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gli3
• Synonyms: add; AI854843; AU023367; Bph; GLI3-190; GLI3FL; Pdn; Xt
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226591 representing NM_008130
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCAGGCCCACAGCTCTACGGCGACTGAGAGGAAGAAAGCTGAAAATTCCATTGGGAAATGTC
  CCACGAGAACAGATGTCAGCGAGAAGGCCGTGGCCTCTAGTACCACTTCCAATGAGGATGAAAGTCCTGG
  ACAGATCTATCACCGAGAGAGAAGAAACGCAATCACTATGCAGCCTCAGAGTGTGCAGGGTCTCAACAAA
  ATCAGTGAGGAGCCCTCCACGTCTAGTGATGAGAGGGCCTCGCTGATCAAGAAAGAGATCCATGGCTCTC
  TACCACATCTGGCGGAGCCCTCTCTCCCTTACCGTGGGACTGTGTTTGCCATGGATCCCCGGAATGGCTA
  CATGGAGCCTCACTACCACCCTCCTCATCTTTTCCCTGCCTTCCATCCTCCTGTACCAATTGATGCCAGA
  CATCATGAGGGCCGTTACCATTATGATCCATCTCCTATTCCTCCATTACATGTGCCTTCTGCCTTATCTA
  GTAGCCCGACGTATCCAGACTTGCCCTTCATTAGGATCTCCCCACACCGTAATCCCACTGCAGCCTCAGA
  GTCCCCCTTCAGCCCCCCACACCCCTACATCAACCCATATATGGACTACATCCGCTCATTGCACAGCAGC
  CCATCCCTCTCCATGATCTCTGCTGCCCGAGGGCTGAGCCCTACAGATGCTCCCCATGCTGGAGTCAGCC
  CTGCGGAATACTATCACCAGATGGCTCTGCTGACAGGCCAGCGCAGCCCTTATGCAGACATCCTTCCCTC
  AGCTGCCACTGCTGGTGCAGGGGCCATCCATATGGAGTACCTTCATGCCATGGACAGCACCAGATTTCCC
  AGCCCTAGGCTGTCAGCTAGGCCCAGCCGAAAGCGCACACTGTCAATATCGCCACTGTCAGATCATAGCT
  TCGACCTTCAGACCATGATAAGAACATCCCCTAACTCCTTGGTTACAATCCTCAATAATTCCCGTAGCAG
  CTCTTCAGCAAGTGGTTCCTATGGGCACTTATCGGCAAGTGCAATCAGCCCTGCATTGAGCTTCACCTAC
  CCCTCCGCTCCTGTGTCTCTTCACATGCATCAACAGATCCTAAGCCGACAGCAAAGCTTAGGCTCCGCAT
  TTGGACACAGCCCTCCTCTCATCCACCCTGCTCCAACATTTCCAACACAGAGACCTATCCCTGGGATTCC
  AACAGTTCTGAACCCCGTCCAGGTCAGCTCTGGCCCTTCTGAGTCCTCACAGAGCAAGCCCACAAGCGAG
  TCTGCAGTGAGCAGCACTGGTGACCCTATGCATAATAAGCGGTCCAAGATCAAGCCTGATGAAGACCTCC
  CCAGTCCAGGGTCACGGGGCCAGCAGGAACAGCCGGAAGGAACAACCCTAGTCAAGGAGGAAGCGGACAA
  AGATGAAAGCAAGCAGGAGCCTGAAGTCATCTACGAGACAAACTGCCACTGGGAAGGCTGCACCAGAGAG
  TTCGACACCCAGGATCAGCTTGTGCATCATATCAATAATGACCACATTCATGGAGAAAAGAAGGAGTTCG
  TGTGCCGCTGGCTTGATTGTTCACGAGAGCAGAAACCGTTCAAAGCCCAGTACATGTTGGTAGTGCATAT
  GAGAAGACACACTGGGGAGAAGCCTCACAAATGTACATTTGAAGGTTGCACAAAAGCCTACTCAAGACTC
  GAAAACCTGAAAACCCACTTGAGATCTCACACTGGAGAGAAGCCATACGTCTGTGAGCATGAGGGCTGCA
  ACAAGGCTTTCTCTAATGCTTCAGATCGGGCCAAGCACCAAAACAGAACACATTCCAATGAGAAACCATA
  TGTATGCAAAATCCCTGGCTGCACCAAGCGTTACACAGACCCCAGCTCTCTCCGGAAACACGTGAAGACT
  GTGCATGGCCCTGAGGCTCATGTTACCAAGAAGCAGCGTGGGGACATGCACCCTCGGCCTCCACCCCCGA
  GAGATTCCGGCAGCCATTCACAGTCCAGGTCCCCTGGCCGGCCAACTCAGGGAGCATTTGGTGAGCAGAA
  GGAGCTGAGCAACACTACCTCAAAGCGGGAAGAATGCCTCCAGGTGAAGACTGTCAAGGCTGAGAAGCCC
  ATGACATCTCAGCCAAGCCCTGGTGGTCAGTCTTCATGCAGCAGCCAACAGTCCCCCATCAGCAACTATT
  CCAACAGTGGGCTCGAGCTTCCTCTGACTGATGGAGGTAGTGTAGCAGACCTCAGTGCCATCGATGAAAC
  CCCAATCATGGACTCGACCATTTCCACGGCAACCACAGCCCTTGCTTTGCAAGCCAGGAGAAACCCGGCA
  GGGACCAAATGGATGGAGCACATCAAACTTGAAAGGCTAAAACAAGTGAATGGAATGTTTCCAAGACTGA
  ACCCTATTCTACCCTCCAAAGCCCCTGCGGTATCTCCTCTCATAGGAAATGGCACACAGTCCAATAACAA
  CTATAGCTCGGGTGGGCCCGGGACCCTCCTCCCGAGCAGAAGTGACCTGTCAGGTGTAGACTTCACAGTG
  CTGAACACACTCAACAGGAGAGACAGCAATACCAGCACTATCAGCTCTGCCTACCTGAGCAGCCGCAGAT
  CCTCGGGCATCTCCCCCTGCTTTTCCAGCCGCAGGTCCAGCGAGGCGTCCCAGGCTGAAGGGCGACCCCA
  GAATGTGAGTGTGGCTGACTCCTACGATCCCATCTCCACAGATGCTTCAAGGAGGTCCAGCGAGGCCAGC
  CAGGGTGACGGGCTGCCCAGTCTGCTCAGCCTCACACCCGTGCAGCAGTACCGCCTTAAGGCCAAGTATG
  CAGCTGCCACAGGTGGCCCACCACCCACACCTCTGCCCCACATGGAGAGGCTGAGCCTGAAGACCAAGAT
  GGCCTTGCTAGGGGAAGGGAGGGACTCTGGGGTGACCCTGCCTCCAGTCCATCCTCCTCGAAGATGCAGT
  GATGGGGGAGGTCACACATACAGAGGGCGTCACCTGATGCCTCATGATGCACTAGCGAACAGTGTGAGGA
  GAGCCAGCGACCCTGTGAGGACAGTCTCGGAGAACATGTCACTTGCCAGGGTTCAACGCTTCAGCAGCCT
  CAATAGCTTTAATCCTCCAAATTTGCCTCCATCGGTGGAAAAGCGTAGCTTAGTGCTGCAAAATTATACC
  CGGCAGGAGAGCAGCCAACCCCGGTACTTCCAGGCATCCCCTTGCCCTCCCAGCATCACAGAGAATGTTG
  CCCTGGAGGCTCTGACCATGGATGCTGATGCTAACTTGAATGATGAAGAACTCCTGCCAGATGATGTGGT
  ACAGTATTTAAATTCCCAGAACCAAACAGGGTATGGGCAGCAGTTGCAGAGTGGCATCTCTGAAGACAGC
  AAAGTAGCCCATGAGCCAGAGGACTTGGACTTAGCAGGGCTGCCAGACAGTCATGTTGGCCAGGAGTACC
  CAGCTCTGGAGCAGCCCTGCTCTGAGGGCAGTAAAACTGATTTGCCCATCCAGTGGAACGAGGTCAGTTC
  TGGAACTTCTGATCTGTCATCCTCCAAGCTGAAGTGTGGTCAGCAGCGCCCTAGCGCACAGCAGCCTCGG
  GGTTTTGGGCTATACAACAATATGGTGGTACACCCACATAATCTGTGGAAGGTTGGCACTGGCCCAGCTG
  GGGGATATCAGACCCTCGGGGAGAATAGCAGTACCTACAATGGCCCAGAACACTTTGCAATCCACAGTGG
  GGATGGACTTGGCACCAATGGAAATACTTTCCATGAACAGCCCTTTAAGACCCAGCAGTATGGGAGCCAG
  CTCAACAGGCAGCCACTGACTTCCAGTGCTCTAGATCATGCCTGTGGTGCAGGGATTCAAGGGTCCAAGC
  TAAAAGGCAACAGCTTGCAAGAGAATGGGGGTTTGCTAGATTTCAGCCTGTCCGTGGCACCAAATGAGTT
  AGCTGGCAACACAGTGAATGGCATGCAAACCCAAGATCAAATGGGACAGGGATACGTTGCCCATCAGCTA
  CTCAGTGGCAGCATGCAACACCAGGGGCCCAGCCGCCCTGGTCAACAGGTACTAGGGCAGGTTGGTGCTA
  CCTCACATATCAACATCTATGAAGGGACAGAGAGCTGCCTACCAGGGACTCAGGACAACAGCAGCCAGCC
  ATCAAGCATGGCAGCTATCAGGGGCTACCAGCCCTGTGCCAGCTATGGGGGTAACAGGCGTCAGGCAATG
  CCAAGGGGCAACCTCACTCTGCAACAAGGACAGCTCAGTGACATGAGTCAGAGCAGCAGGGTGAACAGCA
  TCAAAATGGAGGCACAAGGTCAGTCCCAGCAGCTCTGCTCTACCGTGCAGAATTATTCCGGTCAGTTCTA
  TGACCAAACCATGGGCTTCAGTCAGCAAGACAGGAAAGCTGGCTCATTCTCCCTCTCAGATGCCAACTGC
  CTGCTCCAAGGGAATGGCACTGAAAACTCTGAGTTACTCTCCCCAGGTGCTAACCAGGTAACAAGCACAG
  TTGACAGCTTTGAGAGTCATGACCTAGAAGGTGTGCAGATTGATTTTGATGCCATCATAGATGATGGGGA
  CCATACCAGCCTAATGTCAGGGGCCTTGAGCCCAAGTATTATTCAGAACCTTTCCCACAGCTCCTCCCGT
  CTCACCACTCCGCGGGCATCCCTCCCATTCCCATCCCTATCCATGGGCACCACCAACATGGCTATCGGGG
  ATATGAGTTCTTTGCTGACCTCCCTTGCAGAAGAAAGCAAGTTCCTTGCAGTTATGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226591 representing NM_008130
  Red=Cloning site Green=Tags(s)
  
  MEAQAHSSTATERKKAENSIGKCPTRTDVSEKAVASSTTSNEDESPGQIYHRERRNAITMQPQSVQGLNK
  ISEEPSTSSDERASLIKKEIHGSLPHLAEPSLPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDAR
  HHEGRYHYDPSPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSS
  PSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADILPSAATAGAGAIHMEYLHAMDSTRFP
  SPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTY
  PSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQSKPTSE
  SAVSSTGDPMHNKRSKIKPDEDLPSPGSRGQQEQPEGTTLVKEEADKDESKQEPEVIYETNCHWEGCTRE
  FDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRL
  ENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKT
  VHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKELSNTTSKREECLQVKTVKAEKP
  MTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSVADLSAIDETPIMDSTISTATTALALQARRNPA
  GTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTV
  LNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEAS
  QGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMERLSLKTKMALLGEGRDSGVTLPPVHPPRRCS
  DGGGHTYRGRHLMPHDALANSVRRASDPVRTVSENMSLARVQRFSSLNSFNPPNLPPSVEKRSLVLQNYT
  RQESSQPRYFQASPCPPSITENVALEALTMDADANLNDEELLPDDVVQYLNSQNQTGYGQQLQSGISEDS
  KVAHEPEDLDLAGLPDSHVGQEYPALEQPCSEGSKTDLPIQWNEVSSGTSDLSSSKLKCGQQRPSAQQPR
  GFGLYNNMVVHPHNLWKVGTGPAGGYQTLGENSSTYNGPEHFAIHSGDGLGTNGNTFHEQPFKTQQYGSQ
  LNRQPLTSSALDHACGAGIQGSKLKGNSLQENGGLLDFSLSVAPNELAGNTVNGMQTQDQMGQGYVAHQL
  LSGSMQHQGPSRPGQQVLGQVGATSHINIYEGTESCLPGTQDNSSQPSSMAAIRGYQPCASYGGNRRQAM
  PRGNLTLQQGQLSDMSQSSRVNSIKMEAQGQSQQLCSTVQNYSGQFYDQTMGFSQQDRKAGSFSLSDANC
  LLQGNGTENSELLSPGANQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSR
  LTTPRASLPFPSLSMGTTNMAIGDMSSLLTSLAEESKFLAVMQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008130
• ORF Size: 4749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008130.2, NP_032156.2
• RefSeq Size: 8428
• RefSeq ORF: 4752
• Locus ID: 14634