Wnt7b (NM_009528) Mouse Tagged ORF Clone

CAT#: MG226680

Wnt7b (GFP-tagged) - Mouse wingless-related MMTV integration site 7B (Wnt7b) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt7b
• Synonyms: Wnt-7b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226680 representing NM_009528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGAAACTTTCGAAAGTGGATCTTTTACGTGTTTCTCTGCTTTGGCGTCCTCTACGTGAAGCTCG
  GAGCATTGTCATCCGTGGTGGCCCTGGTAGCCAACATCATCTGCAACAAGATTCCTGGCCTGGCCCCACG
  GCAGCGTGCCATCTGCCAGAGCCGACCCGATGCCATCATTGTGATCGGGGAGGGGGCGCAGATGGGCATC
  GACGAGTGCCAGCACCAGTTCCGATTCGGCCGCTGGAACTGCTCCGCCCTGGGCGAGAAGACCGTCTTCG
  GGCAAGAACTCCGAGTAGGGAGTCGAGAGGCTGCCTTCACCTATGCCATCACGGCGGCGGGCGTGGCGCA
  TGCTGTCACCGCTGCCTGCAGCCAGGGCAATCTGAGCAATTGTGGCTGTGACCGGGAGAAGCAAGGCTAC
  TACAACCAGGCGGAAGGCTGGAAGTGGGGGGGCTGCTCAGCGGACGTCCGCTACGGCATCGACTTTTCTC
  GTCGCTTTGTGGATGCCCGTGAGATCAAAAAGAACGCCAGGCGCCTCATGAACCTTCACAACAATGAGGC
  GGGCAGAAAGGTTCTGGAGGACCGCATGAAGCTGGAATGTAAGTGTCACGGTGTGTCAGGCTCCTGTACC
  ACCAAAACTTGCTGGACCACGCTACCTAAGTTCCGCGAGGTGGGCCACCTGCTCAAGGAGAAGTACAACG
  CAGCGGTGCAGGTGGAGGTGGTGCGAGCCAGCCGCCTGCGCCAGCCCACCTTCCTGCGCATCAAGCAGCT
  ACGCAGCTACCAGAAGCCTATGGAGACGGACCTGGTGTACATCGAGAAGTCGCCCAACTACTGCGAGGAG
  GACGCGGCCACGGGCAGCGTGGGCACGCAGGGCCGTCTGTGCAACCGCACCTCGCCGGGGGCCGACGGCT
  GTGACACCATGTGCTGCGGCCGCGGCTACAACACGCACCAGTACACCAAGGTGTGGCAGTGTAACTGCAA
  ATTCCACTGGTGTTGCTTCGTCAAGTGCAACACGTGCAGCGAGCGCACCGAGGTCTTCACCTGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226680 representing NM_009528
  Red=Cloning site Green=Tags(s)
  
  MHRNFRKWIFYVFLCFGVLYVKLGALSSVVALVANIICNKIPGLAPRQRAICQSRPDAIIVIGEGAQMGI
  DECQHQFRFGRWNCSALGEKTVFGQELRVGSREAAFTYAITAAGVAHAVTAACSQGNLSNCGCDREKQGY
  YNQAEGWKWGGCSADVRYGIDFSRRFVDAREIKKNARRLMNLHNNEAGRKVLEDRMKLECKCHGVSGSCT
  TKTCWTTLPKFREVGHLLKEKYNAAVQVEVVRASRLRQPTFLRIKQLRSYQKPMETDLVYIEKSPNYCEE
  DAATGSVGTQGRLCNRTSPGADGCDTMCCGRGYNTHQYTKVWQCNCKFHWCCFVKCNTCSERTEVFTCK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009528
• ORF Size: 1047 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009528.3, NP_033554.3
• RefSeq Size: 3389
• RefSeq ORF: 1050
• Locus ID: 22422
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. [UniProtKB/Swiss-Prot Function]