Chrna3 (NM_145129) Mouse Tagged ORF Clone

CAT#: MG226689

Chrna3 (GFP-tagged) - Mouse cholinergic receptor nicotinic alpha polypeptide 3 (Chrna3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chrna3
• Synonyms: (a)3; A730007P14Rik; Acra-3; Acra3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226689 representing NM_145129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTCTTCAGACATGGGTGTTGTGCTGCCCCCGCCGCCGCTGTCCATGCTGATGCTGGTGCTGATGC
  TGCTGCCAGTGGCCAGCGCCTCGGAAGCTGAGCACCGCCTGTTCCAGTACCTGTTTGAAGATTACAACGA
  GATCATCCGGCCGGTGGCTAACGTGTCCCATCCTGTCATCATCCAGTTTGAGGTGTCTATGTCTCAGCTG
  GTGAAGGTGGATGAAGTAAACCAGATCATGGAAACCAACCTGTGGCTGAAGCAAATCTGGAATGACTACA
  AGCTGAAATGGAAACCCTCTGACTACCAAGGGGTGGAGTTCATGCGAGTCCCTGCAGAGAAGATCTGGAA
  ACCAGACATCGTGCTTTACAACAACGCCGATGGGGATTTCCAAGTGGATGACAAAACCAAAGCTCTACTC
  AAGTACACAGGAGAAGTGACTTGGATCCCTCCGGCCATCTTTAAGAGCTCATGCAAAATCGATGTGACCT
  ACTTCCCGTTTGACTACCAAAACTGCACCATGAAGTTCGGCTCCTGGTCCTACGACAAGGCAAAGATCGA
  CCTGGTCCTCATTGGCTCCTCCATGAACCTCAAGGACTATTGGGAAAGTGGCGAGTGGGCCATCATTAAA
  GCCCCGGGCTACAAACATGAAATCAAGTACAACTGCTGTGAGGAGATCTACCAAGACATCACGTACTCGC
  TATACATTCGCCGCCTGCCGCTGTTCTACACCATCAACCTCATCATTCCGTGCCTGCTCATCTCCTTCCT
  CACTGTGCTCGTCTTCTACCTGCCCTCCGACTGTGGGGAGAAGGTGACGCTCTGCATCTCCGTGCTCCTC
  TCCCTGACGGTCTTTCTCCTCGTGATCACCGAGACCATCCCTTCCACCTCACTGGTCATCCCCTTGATCG
  GGGAGTACCTCCTCTTCACTATGATTTTTGTCACCTTGTCCATCGTCATCACAGTCTTTGTGCTCAACGT
  GCACTACAGAACTCCGACCACACACACGATGCCCACTTGGGTCAAGGCTGTGTTTTTGAACCTTCTCCCC
  AGGGTCATGTTTATGACTAGGCCAACCAGCACCGAGGAAGACGCCCCAAAGACGAGGAACTTCTACGGTG
  CTGAGCTCTCAAACCTGAACTGCTTCAGCCGTGCAGACTCCAAAAGCTGCAAGGAAGGCTACCCCTGCCA
  AGATGGGACATGTGGCTATTGCCACCACCGTAGGGTAAAAATCTCAAATTTCAGTGCCAACCTCACAAGA
  AGCTCCAGTTCTGAGTCTGTTGATGCTGTGTTGTCCCTGTCTGCTCTGTCACCAGAAATCAAAGAAGCCA
  TCCAAAGTGTGAAGTATATTGCTGAAAATATGAAAGCACAGAATGTAGCCAAAGAGATTCAAGATGATTG
  GAAGTATGTTGCCATGGTGATTGATCGCATTTTTCTCTGGGTTTTCATCCTGGTGTGCATTTTAGGAACT
  GCAGGATTATTTCTGCAACCCTTGATGGCCAGAGATGACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226689 representing NM_145129
  Red=Cloning site Green=Tags(s)
  
  MRSSDMGVVLPPPPLSMLMLVLMLLPVASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQL
  VKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALL
  KYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIK
  APGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLL
  SLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLP
  RVMFMTRPTSTEEDAPKTRNFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTR
  SSSSESVDAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGT
  AGLFLQPLMARDDT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145129
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145129.2, NP_660111.2
• RefSeq Size: 3123
• RefSeq ORF: 1500
• Locus ID: 110834