Was (NM_009515) Mouse Tagged ORF Clone

CAT#: MG226777

Was (GFP-tagged) - Mouse Wiskott-Aldrich syndrome homolog (human) (Was), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Was
• Synonyms: U42471; Wasp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226777 representing NM_009515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAGTGGCCCTGGCCCTGTAGGAGGCAGGCCTGGGGGCCGAGGGGGACCAGCCGTTCAGCAGAACA
  TTCCTTCCAACCTCCTCCAGGACCATGAAAACCAGAGACTCTTTGAGCTTCTTGGCCGAAAATGCTGGAC
  ACTGGCTACCACAGTTGTTCAGCTCTACCTGGCACTGCCCCCTGGAGCTGAGCACTGGACCATGGAACAC
  TGCGGGGCTGTGTGCTTCGTGAAGGATAACCCTCAGAAGTCCTACTTCATCCGCCTTTATGGCCTACAGG
  CTGGTCGGCTACTCTGGGAACAGGAGCTGTACTCTCAGCTGGTTTATCTCACTCCCACCCCGTTCTTCCA
  CACTTTTGCTGGAGATGACTGTCAAGTAGGACTGAACTTTGCGGATGAGAGTGAAGCCCAGGCCTTCCGG
  GCCTTGGTGCAGGAGAAGATACAAAAAAGGAATCAGAGGCAAAGCGGAGAAAGACGCCAGCTACCACCAC
  CACCAGCACCAATCAATGAGGAGAGAAGAGGAGGGCTCCCACCTGTGCCCCCACACCCGGGTGGAGATCA
  TGGGGGCCCATCAGGTGGTCCACTATCTCTAGGACTTGTGACGGTCGACATTCAGAACCCTGACATCACA
  AGTTCACGATACCGTGGGCTCCCTGCACCTGGCCCTGGCCCAACTGATAAGAAACGCTCAGGGAAAAAGA
  AGATCAGCAAAGCTGATATCGGAGCACCGAGTGGATTCAAACATGTCAGCCACGTGGGCTGGGATCCCCA
  GAATGGATTTGATGTGAACAACCTAGACCCGGATCTGCGGAGCTTGTTCTCCAGGGCAGGAATCAGCGAG
  GCCCAGCTCACTGACGCAGAGACCTCCAAGCTCATCTACGATTTTATTGAGGACCAGGGAGGTCTAGAGG
  CTGTCCGGCAGGAGATGAGGCGCCAAGAGCCACTCCCACCACCTCCGCCGCCATGCAGAGGAGGAGGAGG
  AGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCCAGCCTCTGAGACCTCCTGTTGTGGGG
  AGTAATAAGGGTCGCTCAGGTCCACTGCCCCCTGTACCTATGGGGGGTGCCCCACCTCCACCAACACCAC
  GAGGGCCCCCACCACCAGGCCGAGGGGGTCCTCCTCCACCACCCCCTCCAGCCACTGGACGATCTGGACC
  ACCACCTCCTCCACTCCCTGGAGCTGGGGGACCACCAGCACCGCCACCACCACCACCACCACCACCACCT
  CCACCCTGCCCTGGGAGTGGGCCCGCCCCTCCCCCGCTCCCTCCTACTCCAGTGTCTGGGGGGAGCCCAG
  CACCTGGTGGGGGCCGGGGTGCACTTTTGGACCAAATCCGGCAGGGAATTCAGCTGAACAAGACCCCTGG
  AGCTCTAGAGAACTCAGTACAGCAACCACCCGCGCAGCAGTCAGAAGGCCTAGTAGGTGCCCTGATGCAT
  GTCATGCAGAAGAGGAGTAGAGTCATCCATTCCTCAGATGAAGGGGAGGATCAGACCGGCGAGGATGAAG
  AGGATGATGAATGGGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226777 representing NM_009515
  Red=Cloning site Green=Tags(s)
  
  MNSGPGPVGGRPGGRGGPAVQQNIPSNLLQDHENQRLFELLGRKCWTLATTVVQLYLALPPGAEHWTMEH
  CGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYLTPTPFFHTFAGDDCQVGLNFADESEAQAFR
  ALVQEKIQKRNQRQSGERRQLPPPPAPINEERRGGLPPVPPHPGGDHGGPSGGPLSLGLVTVDIQNPDIT
  SSRYRGLPAPGPGPTDKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISE
  AQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPCRGGGGGGGGGGGGGGGGGGQPLRPPVVG
  SNKGRSGPLPPVPMGGAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPPPPPLPGAGGPPAPPPPPPPPPP
  PPCPGSGPAPPPLPPTPVSGGSPAPGGGRGALLDQIRQGIQLNKTPGALENSVQQPPAQQSEGLVGALMH
  VMQKRSRVIHSSDEGEDQTGEDEEDDEWDD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009515
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009515.2, NP_033541.1
• RefSeq Size: 2094
• RefSeq ORF: 1563
• Locus ID: 22376
• Gene Summary: Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. [UniProtKB/Swiss-Prot Function]