Smad9 (NM_019483) Mouse Tagged ORF Clone

CAT#: MG226782

Smad9 (GFP-tagged) - Mouse MAD homolog 9 (Drosophila) (Smad9), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smad9
• Synonyms: MADH6; Madh8; Madh9; Smad8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226782 representing NM_019483
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCCAGCACCCCCATCAGCTCCCTCTTCTCCTTCACCAGCCCCGCAGTGAAGCGGCTGCTGGGCT
  GGAAGCAGGGAGATGAAGAGGAGAAGTGGGCAGAGAAGGCGGTGGACTCTTTGGTGAAGAAGTTAAAGAA
  GAAGAAAGGCGCCATGGATGAACTGGAGAGGGCGCTGAGCTGCCCGGGTCAGCCTAGCAAGTGTGTCACC
  ATCCCACGGTCCCTCGATGGACGCCTCCAGGTGTCCCACCGAAAGGGGCTGCCCCACGTCATCTACTGCC
  GCGTGTGGCGCTGGCCAGACCTGCAGTCCCATCATGAGCTGAAGCCCTTGGAGTGCTGTGAGTTCCCGTT
  CGGCTCCAAGCAGAAGGAGGTCTGCATCAACCCATACCATTACCGCAGAGTGGAGACCCCAGTTCTGCCT
  CCAGTGCTGGTACCAAGACACAGCGAGTACAACCCTCAGCTCAGCCTCCTGGCCAAGTTCCGAAGTGCCT
  CGCTGCACAGCGAACCCCTCATGCCGCACAACGCCACCTACCCTGACTCTTTCCAGCAGTCTCTCTGTCC
  GGCACCGCCCTCCTCGCCAGGCCATGTGTTTCCGCAGTCTCCATGCCCCACCAGCTACCCGCACTCCCCC
  GGAAGTCCTTCAGACAGTCCCTATCAACACTCAGACTTCCGGCCAGTTTGCTACGAGGAACCCCAGCACT
  GGTGTTCTGTTGCCTACTACGAACTAAACAACCGGGTCGGAGAGACTTTCCAGGCGTCCTCGCGGAGCGT
  GCTCATAGACGGCTTCACCGACCCTTCCAATAACAGGAATAGGTTTTGCCTTGGGCTTCTCTCAAATGTA
  AACAGAAACTCGACCATAGAAAACACCAGGAGGCACATTGGAAAGGGTGTGCATTTGTACTACGTTGGGG
  GCGAGGTGTATGCGGAGTGCGTGAGCGACAGCAGCATCTTTGTCCAGAGCCGGAACTGCAACTACCAGCA
  CGGCTTCCACCCGGCCACCGTCTGCAAGATCCCCAGCGGCTGCAGCCTCAAGGTCTTCAACAACCAGCTC
  TTCGCCCAGCTGCTCGCCCAGTCCGTGCACCACGGCTTTGAAGTGGTGTATGAGCTGACGAAGATGTGCA
  CGATTCGGATGAGCTTTGTGAAGGGCTGGGGAGCAGAGTATCATCGCCAGGATGTCACGAGCACCCCCTG
  CTGGATCGAGATCCATCTTCATGGACCGCTGCAGTGGTTGGATAAGGTGCTCACTCAGATGGGCTCCCCA
  CACAACCCTATCTCTTCAGTGTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226782 representing NM_019483
  Red=Cloning site Green=Tags(s)
  
  MHPSTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALSCPGQPSKCVT
  IPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLP
  PVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDSFQQSLCPAPPSSPGHVFPQSPCPTSYPHSP
  GSPSDSPYQHSDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFCLGLLSNV
  NRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPATVCKIPSGCSLKVFNNQL
  FAQLLAQSVHHGFEVVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSP
  HNPISSVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019483
• ORF Size: 1290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019483.4, NP_062356.3
• RefSeq Size: 5368
• RefSeq ORF: 1293
• Locus ID: 55994
• Gene Summary: This gene encodes a member of a family of proteins that act as downstream effectors of the bone morphogenetic protein (BMP) signaling pathway. The encoded protein is phosphorylated by BMP receptors, which stimulates its binding to SMAD4 and translocation into the nucleus, where it functions as a regulator of transcription. Activity of this protein is important for embryonic development. Mutation of this gene results in defects in pulmonary vasculature. [provided by RefSeq, Mar 2013]