Nfkb1 (NM_008689) Mouse Tagged ORF Clone

CAT#: MG226792

Nfkb1 (GFP-tagged) - Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 p105 (Nfkb1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfkb1
• Synonyms: NF-kappaB; NF-kappaB1; NF-KB1; p50; p50/p105; p105
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226792 representing NM_008689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACGATGATCCCTACGGAACTGGGCAAATGTTTCATTTGAACACTGCTTTGACTCACTCAATAT
  TTAATGCAGAATTATATTCACCAGAAATACCACTGTCAACAGATGGCCCATACCTTCAAATATTAGAGCA
  ACCAAAACAGAGGGGATTTCGATTCCGCTATGTGTGTGAAGGCCCATCACACGGAGGGCTTCCGGGAGCC
  TCTAGTGAGAAGAACAAGAAATCCTACCCACAGGTCAAAATTTGCAACTATGTGGGGCCTGCAAAGGTTA
  TCGTTCAGTTGGTCACAAATGGAAAAAACATCCACCTGCACGCCCACAGCCTGGTGGGCAAGCACTGTGA
  GGACGGGGTATGCACCGTAACAGCAGGACCCAAGGACATGGTGGTTGGCTTTGCAAACCTGGGAATACTT
  CATGTGACTAAGAAAAAGGTATTTGAAACACTGGAAGCACGGATGACAGAGGCGTGTATTAGGGGCTATA
  ATCCTGGACTTCTGGTGCATTCTGACCTTGCCTATCTACAAGCAGAAGGCGGAGGAGACCGGCAACTCAC
  AGACAGAGAGAAGGAGATCATCCGCCAGGCAGCCGTGCAGCAGACCAAGGAGATGGACCTGAGCGTGGTG
  CGCCTCATGTTCACAGCCTTCCTCCCTGACAGCACTGGCAGCTTCACTCGGAGACTGGAGCCTGTGGTGT
  CAGACGCCATCTATGATAGCAAAGCCCCGAATGCATCCAACCTGAAAATCGTGAGAATGGACAGAACAGC
  AGGATGTGTGACGGGAGGGGAGGAGATTTACCTTCTCTGTGACAAGGTTCAGAAAGATGACATCCAGATT
  CGGTTTTATGAAGAGGAAGAAAATGGCGGAGTTTGGGAAGGATTTGGGGACTTTTCCCCCACAGATGTTC
  ATAGACAGTTTGCCATTGTCTTCAAAACGCCAAAGTATAAGGATGTCAACATTACAAAGCCAGCTTCCGT
  GTTTGTTCAGCTTCGGAGGAAATCAGACCTGGAAACTAGTGAACCGAAACCCTTTCTCTACTACCCTGAA
  ATCAAAGACAAAGAGGAAGTGCAAAGGAAACGCCAGAAGCTTATGCCGAACTTCTCGGACAGCTTCGGTG
  GCGGCAGTGGAGCGGGAGCCGGTGGTGGAGGCATGTTCGGTAGTGGCGGTGGCGGAGGGAGTACCGGAAG
  CCCTGGCCCAGGGTATGGCTACTCGAACTACGGATTTCCTCCCTACGGTGGGATTACATTCCATCCCGGA
  GTCACGAAATCCAACGCAGGGGTCACCCATGGCACCATAAACACCAAATTTAAAAATGGCCCTAAAGATT
  GTGCCAAGAGTGATGACGAGGAGAGTCTGACTCTCCCTGAGAAGGAAACTGAAGGTGAAGGGCCCAGCCT
  GCCCATGGCCTGCACCAAGACGGAACCCATCGCCTTGGCATCCACCATGGAAGACAAGGAGCAGGACATG
  GGATTTCAGGATAACCTCTTTCTTGAGAAGGCTCTGCAGCTCGCCAGGCGACACGCCAACGCCCTTTTCG
  ACTACGCAGTGACGGGGGATGTGAAGATGTTGCTGGCCGTGCAACGCCATCTCACCGCCGTGCAGGATGA
  GAATGGGGACAGTGTCTTACACTTAGCCATCATCCACCTCCACGCTCAGCTTGTGAGGGATCTGCTGGAA
  GTCACATCTGGTTTGATCTCTGATGACATCATCAACATGAGAAATGACCTGTATCAGACACCTCTGCACT
  TGGCCGTGATCACCAAGCAGGAAGATGTAGTAGAGGATTTGCTGAGGGTTGGGGCTGACCTGAGCCTTCT
  GGACCGCTGGGGCAACTCTGTCCTGCACCTAGCTGCCAAAGAAGGACACGACAGAATCCTCAGCATCCTG
  CTCAAGAGCAGAAAAGCAGCGCCCCTTATCGACCACCCCAATGGGGAAGGTCTAAATGCCATCCACATAG
  CTGTGATGAGCAATAGCCTGCCATGTCTGCTGCTGCTGGTGGCTGCCGGGGCAGAAGTCAATGCTCAGGA
  GCAGAAGTCTGGGCGCACAGCGCTGCACCTGGCCGTGGAGTACGACAACATCTCCTTGGCTGGCTGCCTG
  CTTCTGGAGGGTGATGCCCACGTGGACAGTACCACCTATGATGGGACTACACCTCTGCATATAGCGGCCG
  GAAGAGGGTCCACCAGACTGGCAGCTCTTCTCAAAGCAGCAGGAGCAGACCCCCTGGTGGAGAACTTTGA
  GCCTCTCTATGACCTGGACGACTCTTGGGAGAAGGCTGGAGAAGATGAGGGAGTGGTGCCAGGTACCACA
  CCCCTGGACATGGCTGCCAACTGGCAGGTATTTGACATACTAAATGGGAAACCGTATGAGCCTGTGTTCA
  CATCTGATGATATACTACCACAAGGGGACATGAAGCAGCTGACAGAAGACACGAGGCTACAACTCTGCAA
  ACTGCTGGAAATTCCTGATCCAGACAAAAACTGGGCCACTCTGGCACAGAAGTTGGGTCTGGGGATACTG
  AACAATGCCTTCCGGCTGAGTCCTGCTCCTTCTAAAACTCTCATGGACAACTATGAGGTCTCTGGGGGTA
  CCATCAAAGAGCTGATGGAGGCCCTGCAACAGATGGGCTACACAGAGGCCATTGAAGTGATCCAGGCAGC
  CTTCCGCACCCCGGCAACCACAGCCTCCAGCCCCGTGACCACTGCTCAGGTCCACTGTCTGCCTCTCTCG
  TCTTCCTCCACGAGGCAGCACATAGATGAACTCCGGGATAGTGACAGCGTCTGTGACAGTGGTGTGGAGA
  CATCCTTCCGCAAACTCAGCTTTACAGAGTCTCTTACTGGAGACAGCCCACTGCTATCTCTGAACAAAAT
  GCCCCACGGTTATGGGCAGGAAGGACCTATTGAAGGCAAAATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226792 representing NM_008689
  Red=Cloning site Green=Tags(s)
  
  MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGA
  SSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGIL
  HVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVV
  RLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQI
  RFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPE
  IKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPG
  VTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTLPEKETEGEGPSLPMACTKTEPIALASTMEDKEQDM
  GFQDNLFLEKALQLARRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHAQLVRDLLE
  VTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRVGADLSLLDRWGNSVLHLAAKEGHDRILSIL
  LKSRKAAPLIDHPNGEGLNAIHIAVMSNSLPCLLLLVAAGAEVNAQEQKSGRTALHLAVEYDNISLAGCL
  LLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTT
  PLDMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGLGIL
  NNAFRLSPAPSKTLMDNYEVSGGTIKELMEALQQMGYTEAIEVIQAAFRTPATTASSPVTTAQVHCLPLS
  SSSTRQHIDELRDSDSVCDSGVETSFRKLSFTESLTGDSPLLSLNKMPHGYGQEGPIEGKI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008689
• ORF Size: 2913 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008689.2, NP_032715.2
• RefSeq Size: 4128
• RefSeq ORF: 2916
• Locus ID: 18033
• Gene Summary: Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-p50 complex. Homodimer; component of the NF-kappa-B p50-p50 complex. Component of the NF-kappa-B p105-p50 complex. Component of the NF-kappa-B p50-c-Rel complex. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. NFKB1/p105 interacts with CFLAR; the interaction inhibits p105 processing into p50. NFKB1/p105 forms a ternary complex with MAP3K8 and TNIP2. Interacts with GSK3B; the interaction prevents processing of p105 to p50. NFKB1/p50 interacts with NFKBIE (By similarity). NFKB1/p50 interacts with NFKBIZ. Nuclear factor NF-kappa-B p50 subunit interacts with NFKBID. Directly interacts with MEN1 (By similarity). Interacts with HIF1AN. [UniProtKB/Swiss-Prot Function]