Pld1 (NM_008875) Mouse Tagged ORF Clone

CAT#: MG226799

Pld1 (GFP-tagged) - Mouse phospholipase D1 (Pld1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pld1
• Synonyms: AA536939; C85393; mPLD1; Pld1a; Pld1b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226799 representing NM_008875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTGAAAAGCGAGACCAGGGTGAACACGTCTACACTGCAGAAAATCGCCGCAGATATGAGCAACC
  TGATAGAAAATCTCGACACCCGGGAGCTTCATTTTGAGGGAGAGGAGGTGGAATATGACGCATCTCCAGG
  AGACCCCAAGGCCCAGGAAGGGTGCATCCCATTCTCTTCTATTTACAACACTCAAGGATTTAAGGAGCCC
  AACATCCAGACCTACCTCTCTGGCTGTCCCATAAAAGCACAAGTACTGGAAGTGGAGCGCTTCACATCGA
  CCTCAAGGGTGCCAAGTATCAACCTGTACACGATCGAGTTAACGCACGGGGAATTCACATGGCAAGTTAA
  GAGGAAATTCAAGCACTTCCAAGAGTTTCACAGAGAGCTGCTCAAGTACAAAGCCTTCATCCGGATCCCC
  ATCCCCACTAAAAGACATACATTTAGAAGACAAAATGTCAAAGAAGAACCCCGAGAGATGCCCAGTCTGC
  CCCGTTCATCAGAAAACGCAATCCAGGAAGAGCAGTTCTTTGGCAGGAGGAAGCAACTGGAAGATTACTT
  GACAAAAATACTAAAAATGCCCATGTACAGAAACTATCATGCCACGACAGAGTTCCTTGATGTAAGCCAG
  CTGTCCTTCATCCATGATCTGGGACCAAAGGGCCTGGAAGGAATGATCATGAAAAGGTCTGGGGGACACA
  GGATACCAGGTGTGAATTGCTGTGGCCATGGACGAGCCTGCTACCGGTGGTCAAAGAGGTGGTTGATAGT
  AAAAGACTCTTTCCTGCTCTATATGAAACCAGATAGCGGGGCTATTGCCTTCGTCCTCCTTGTAGATAAA
  GAATTCAGAGTCAAAGTGGGAAGGAAAGAGACAGAAACGAAATATGGACTTCGGATCGATAATCTTTCAA
  GGACCCTGATTTTAAAATGTAACAGCTACAGACACGCCCGGTGGTGGGGAGGCGCCATCGAGGAGTTCAT
  CCGGAAGCATGGAGCTGACTTCCTCAAAGACCATCGCTTCGGGTCCTACGCCGCTCTCCATGAGAACACG
  TTAGCTAAGTGGTATGTGAACGCCAAAGGTTACTTTGAAGACATCGCAAATGCAATGGAAGAGGCATCGG
  AAGAGATTTTTATCACAGATTGGTGGTTAAGTCCAGAAATCTTCCTGAAGCGCCCAGTGGTGGAAGGCAA
  CCGCTGGAGGCTGGACTGCATCCTCAAACGGAAAGCACAACAAGGTGTGCGGATCTTCATAATGCTTTAC
  AAAGAGGTGGAGCTAGCCCTCGGGATCAACAGCGAGTACAGCAAGCGGACGTTGATGAGGCTGCACCCCA
  ACATAAAGGTGATGAGACACCCAGACCACGTGTCCTCCAGCGTGTACCTGTGGGCTCACCATGAGAAGCT
  TGTCATTATCGACCAATCAGTGGCTTTCGTGGGTGGGATTGACCTGGCCTACGGAAGGTGGGATGACAAT
  GAGCACAGACTCACAGACGTGGGCAGTGTCAAGCGGGTCACCTCAGGACTGTCTCTGGGCTCCCTCACGG
  CAGCATCAGTAGAGTCTATGGAATCCTTAAGCCTCAAGGATAAACATGAATTTCATAAAAAGGAGCCCAT
  CTCAAAGATTGTTGATGAGACTGACATGAAGCTGAAAGGGATAGGAAAGTCCAGGAAATTCTCCAAATTT
  AGTCTCTATCGCCAGCTGCACCGTCACCACCTGCACAACGCGGACAGCATCAGCAGCATCGACAGCACCT
  CCAACACTGGCTCCATCCGCAGCGTGCAGACCGGAGTGGGAGAACTCCACGGGGAGACTAGGTTTTGGCA
  TGGGAAGGATTACTGCAACTTTGTCTTCAAGGACTGGGTTCAACTGGACAAACCTTTTGCTGATTTCATT
  GACAGGTACTCAACCCCCCGGATGCCTTGGCATGATATTGGCTCTGTGGTCCATGGAAAGGCAGCTCGGG
  ATGTGGCTCGACACTTCATCCAGCGCTGGAACTTCACGAAGATTATGAAGCCAAAATACCGCTCCCTTTC
  TTATCCCTTCCTGCTACCAAAGTCTCAAGCGACAGCACACGAGTTGAGATATCAGGTGCCAGGGGCCGTC
  CCCGCCAAAGTGCAGTTGCTCCGATCTGCTGCTGATTGGTCTGCTGGTATAAAGCACCATGAGGAATCCA
  TCCACGCTGCCTACATCCATGTAATAGAGAACAGCAAGCACTACATCTATATTGAAAACCAATTTTTCAT
  AAGCTGTGCTGATGATAAAGTTGTGTTCAACAAGGTGGGAGATGCTATCGCCCAGAGAATCCTGAAAGCC
  CACAGGGAAGGCCAGAGATATCGGGTGTACATTGTGATACCGCTGCTGCCGGGATTCGAGGGAGACATTT
  CCACAGGCGGGGGGAACGCTCTACAGGCAATAATGCACTTCAACTACAGAACCATGTGCAGAGGGGAGAG
  CTCCATCCTTGAACAGTTAAAGCCAGAGCTTGGTAATAAGTGGATAAATTACATATCGTTTTGTGGACTG
  AGAACACATGCAGAGCTGGAAGGAAACCTAGTCACCGAGCTCATCTACGTCCACAGCAAGTTGTTAATTG
  CTGACGACAACACAGTTATTATTGGTTCTGCCAACATCAATGACCGGAGCATGCTGGGGAAGCGAGACAG
  CGAAATGGCTGTCATAGTGCAGGACACAGAGACGGTCCCATCGGTGATGGATGGAAAGGAATATCAGGCT
  GGTCGCTTTGCCCGAGACCTTCGCCTGGAGTGCTTCAGACTTGTCCTGGGTTATCTTTCTGACCCAAGTG
  AGGACCTTCAGGATCCTGTGAGTGACAAATTCTTCAAGGAGATTTGGGTTTCAACAGCAGCTCGAAACGC
  TACCATATATGATAAGGTCTTCCGGTGCCTTCCCAATGATGAAGTACACAATTTAATCCAGCTGCGGGAC
  TTTATAAACAAGCCCATATTAGCAAAAGAGGATCGCCTTCGCGCCGAGGAAGAACTGAGGAAGATCCGCG
  GGTTCTTGGTGCAGTTCCCTCTCTATTTCTTATCAGAAGAGAACCTGCTGCCTTCTGTTGGAACCAAAGA
  AGCCATTGTGCCTATGGAGGTTTGGACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226799 representing NM_008875
  Red=Cloning site Green=Tags(s)
  
  MSLKSETRVNTSTLQKIAADMSNLIENLDTRELHFEGEEVEYDASPGDPKAQEGCIPFSSIYNTQGFKEP
  NIQTYLSGCPIKAQVLEVERFTSTSRVPSINLYTIELTHGEFTWQVKRKFKHFQEFHRELLKYKAFIRIP
  IPTKRHTFRRQNVKEEPREMPSLPRSSENAIQEEQFFGRRKQLEDYLTKILKMPMYRNYHATTEFLDVSQ
  LSFIHDLGPKGLEGMIMKRSGGHRIPGVNCCGHGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDK
  EFRVKVGRKETETKYGLRIDNLSRTLILKCNSYRHARWWGGAIEEFIRKHGADFLKDHRFGSYAALHENT
  LAKWYVNAKGYFEDIANAMEEASEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLY
  KEVELALGINSEYSKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDN
  EHRLTDVGSVKRVTSGLSLGSLTAASVESMESLSLKDKHEFHKKEPISKIVDETDMKLKGIGKSRKFSKF
  SLYRQLHRHHLHNADSISSIDSTSNTGSIRSVQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFI
  DRYSTPRMPWHDIGSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQATAHELRYQVPGAV
  PAKVQLLRSAADWSAGIKHHEESIHAAYIHVIENSKHYIYIENQFFISCADDKVVFNKVGDAIAQRILKA
  HREGQRYRVYIVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGESSILEQLKPELGNKWINYISFCGL
  RTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQA
  GRFARDLRLECFRLVLGYLSDPSEDLQDPVSDKFFKEIWVSTAARNATIYDKVFRCLPNDEVHNLIQLRD
  FINKPILAKEDRLRAEEELRKIRGFLVQFPLYFLSEENLLPSVGTKEAIVPMEVWT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008875
• ORF Size: 3108 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008875.4, NP_032901.2
• RefSeq Size: 4932
• RefSeq ORF: 3111
• Locus ID: 18805