Prkaa2 (NM_178143) Mouse Tagged ORF Clone

CAT#: MG226994

Prkaa2 (GFP-tagged) - Mouse protein kinase AMP-activated alpha 2 catalytic subunit (Prkaa2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prkaa2
• Synonyms: 2310008I11Rik; A830082D05; AMPKalpha2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226994 representing NM_178143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGACACTACGTCCTGGGGGACACCCTGGGCG
  TCGGCACCTTCGGCAAAGTGAAGATTGGAGAACACCAATTGACAGGCCATAAAGTGGCAGTTAAGATCTT
  AAATAGACAGAAGATTCGCAGTTTAGATGTTGTTGGAAAAATAAAACGAGAAATTCAAAATCTTAAACTC
  TTTCGTCATCCTCATATTATCAAACTCTACCAGGTGATCAGCACTCCGACAGACTTTTTTATGGTAATGG
  AATATGTGTCTGGAGGTGAATTGTTCGACTACATCTGCAAACATGGGCGGGTTGAAGAGGTGGAAGCGCG
  CCGGCTCTTCCAGCAGATCCTGTCTGCCGTGGATTACTGTCACAGGCATATGGTTGTCCATAGGGACCTG
  AAGCCAGAGAATGTGCTGCTGGATGCCCAGATGAACGCTAAGATAGCTGACTTTGGACTATCTAATATGA
  TGTCAGATGGTGAATTTCTACGAACTAGCTGTGGATCGCCAAATTATGCAGCACCTGAGGTCATCTCAGG
  AAGGCTGTATGCAGGTCCCGAGGTCGATATCTGGAGCTGTGGTGTCATCCTGTATGCCCTTCTCTGTGGC
  ACCCTCCCTTTCGATGATGAGCACGTGCCTACGCTCTTCAAGAAGATCCGAGGGGGTGTGTTTTACATCC
  CAGACTATCTCAACCGTTCTGTCGCCACTCTGCTGATGCACATGCTCCAGGTGGACCCCCTGAAGCGAGC
  GACTATCAAAGACATACGAGAACATGAATGGTTTAAACAGGATTTGCCCAGCTACCTATTTCCTGAAGAC
  CCCTCCTACGATGCGAATGTCATTGACGATGAGGCTGTGAAGGAAGTCTGTGAGAAATTCGAGTGTACAG
  AGTCAGAAGTGATGAATAGTCTGTATAGTGGTGACCCTCAAGACCAGCTTGCAGTGGCTTATCATCTTAT
  CATTGACAATCGGAGAATAATGAACCAAGCCAGTGAGTTCTACCTCGCCTCTAGTCCTCCATCAGGTTCT
  TTTATGGATGACAGCGCCATGCATATTCCTCCAGGCTTGAAACCACATCCAGAAAGGATGCCGCCTCTCA
  TCGCAGACAGCCCCAAAGCACGCTGTCCACTGGATGCACTCAACACAACGAAGCCCAAGTCTCTAGCTGT
  GAAAAAAGCCAAGTGGCATCTTGGAATCCGAAGCCAGAGCAAAGCGTGTGACATTATGGCTGAAGTGTAC
  CGAGCTATGAAGCAGCTGGGTTTTGAATGGAAGGTAGTGAATGCATACCATCTTCGAGTAAGAAGAAAAA
  ACCCAGTGACTGGCAACTATGTGAAAATGAGCTTACAGCTTTACCTGGTAGACAGTCGGAGCTATCTTCT
  GGACTTCAAAAGCATCGATGATGAGGTGGTGGAGCAGAGGTCTGGTTCTTCAACACCCCAGCGCTCCTGT
  TCTGCTGCGGGCCTCCACAGAGCACGGTCAAGTTTTGATTCCAGCACAGCTGAGAACCACTCCCTTTCTG
  GCTCTCTCACTGGCTCTTTGACTGGCAGCACTTTGTCCTCGGCATCCCCGCGCCTGGGCAGTCACACCAT
  GGATTTTTTTGAAATGTGCGCCAGTCTTATCACTGCTTTAGCTCGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226994 representing NM_178143
  Red=Cloning site Green=Tags(s)
  
  MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKL
  FRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDL
  KPENVLLDAQMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCG
  TLPFDDEHVPTLFKKIRGGVFYIPDYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPED
  PSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGS
  FMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKACDIMAEVY
  RAMKQLGFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDSRSYLLDFKSIDDEVVEQRSGSSTPQRSC
  SAAGLHRARSSFDSSTAENHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITALAR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178143
• ORF Size: 1656 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178143.2, NP_835279.2
• RefSeq Size: 8201
• RefSeq ORF: 1659
• Locus ID: 108079