Nodal (NM_013611) Mouse Tagged ORF Clone

CAT#: MG226998

Nodal (GFP-tagged) - Mouse nodal (Nodal), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nodal
• Synonyms: Tg.413d
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226998 representing NM_013611
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCCCACAGCCTCCGCATCCTTCTTCTTCAAGCCTGTTGGGCTCTACTCCACCCGCGCGCCCCGA
  CCGCGGCCGCTTTGCCTCTGTGGACACGGGGGCAGCCCTCGTCACCGTCCCCTCTGGCGTACATGTTGAG
  CCTCTACCGAGACCCGCTGCCTCGGGCGGACATCATCCGCAGCCTCCAGGCGCAAGATGTGGACGTGACC
  GGACAGAACTGGACTTTCACGTTTGACTTCTCTTTTTTGAGCCAAGAAGAGGATCTGGTATGGGCGGAGC
  TCCGGTTGCAGCTGCCGGGCCCCATGGACATACCCACTGAGGGCCCACTCACCATTGACATTTTCCACCA
  GGCCAAGGGGGATCCAGAGCGGGACCCCGCTGACTGCCTGGAGCGCATTTGGATGGAGACGTTCACCGTC
  ATTCCTTCTCAGGTCACGTTTGCCTCAGGCAGCACAGTCCTGGAGGTGACCAAGCCACTCTCCAAGTGGC
  TAAAGGACCCCAGGGCACTGGAAAAGCAGGTGTCCAGTCGAGCAGAAAAGTGTTGGCATCAGCCCTACAC
  CCCACCTGTACCTGTCGCCAGCACCAATGTGCTCATGCTCTACTCCAACCGGCCTCAGGAGCAGAGGCAG
  CTAGGGGGCGCCACTTTGCTTTGGGAAGCTGAGAGCTCCTGGCGGGCCCAGGAGGGACAGCTGTCTGTAG
  AGAGGGGCGGATGGGGCAGAAGGCAACGCCGACATCATTTGCCAGACAGAAGCCAACTGTGTAGGAGGGT
  CAAGTTCCAGGTGGACTTCAACCTGATTGGCTGGGGCTCCTGGATCATCTACCCCAAGCAGTACAATGCC
  TATCGCTGTGAGGGCGAGTGTCCTAACCCTGTGGGGGAGGAGTTTCATCCTACCAACCATGCCTACATCC
  AGAGCCTGCTGAAACGATACCAACCCCACCGGGTTCCTTCCACGTGCTGTGCCCCCGTGAAGACCAAGCC
  ACTGAGCATGCTTTATGTGGACAATGGCAGGGTCCTCCTGGAACACCACAAGGACATGATTGTGGAGGAG
  TGTGGGTGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226998 representing NM_013611
  Red=Cloning site Green=Tags(s)
  
  MSAHSLRILLLQACWALLHPRAPTAAALPLWTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAQDVDVT
  GQNWTFTFDFSFLSQEEDLVWAELRLQLPGPMDIPTEGPLTIDIFHQAKGDPERDPADCLERIWMETFTV
  IPSQVTFASGSTVLEVTKPLSKWLKDPRALEKQVSSRAEKCWHQPYTPPVPVASTNVLMLYSNRPQEQRQ
  LGGATLLWEAESSWRAQEGQLSVERGGWGRRQRRHHLPDRSQLCRRVKFQVDFNLIGWGSWIIYPKQYNA
  YRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLEHHKDMIVEE
  CGCL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013611
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013611.4, NP_038639.2
• RefSeq Size: 2094
• RefSeq ORF: 1065
• Locus ID: 18119
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. Homozygous knockout mice for this gene exhibit early embryonic lethality, while expression of a hypomorphic allele results in defects in anteroposterior and left-right patterning. [provided by RefSeq, Aug 2016]