Ern1 (NM_023913) Mouse Tagged ORF Clone

CAT#: MG226999

Ern1 (GFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ern1
• Synonyms: 9030414B18Rik; AI225830; C85377; Ire1a; Ire1alpha; Ire1p
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226999 representing NM_023913
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCCGGTGGCTGTTGCTCCTGCTGGCGCTGCTGCTACCGCCGCCCGGCCCCGGGAGTTTTGGAA
  GAACCAGCACAGTTACACTGCCTGAGACCTTGTTGTTTGTCTCGACCCTGGATGGAAGCTTGCATGCTGT
  TAGCAAGAGGACGGGCTCCATCAAGTGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACACGTG
  GAAGAGCCGGCTTTCCTCCCAGATCCCAATGATGGCAGTCTGTACACACTTGGAGGCAAGAACAACGAAG
  GCCTGACGAAACTTCCCTTTACCATCCCAGAATTGGTTCAGGCCTCCCCATGCCGAAGTTCAGATGGAAT
  CCTCTACATGGGTAAAAAGCAAGATATTTGGTATGTTATCGACCTCCTGACTGGCGAGAAGCAGCAGACT
  TTGTCATCGGCCTTTGCTGATAGTCTCTGCCCATCAACTTCCCTTCTATATCTTGGACGGACAGAATACA
  CCATCACCATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACCTATTTTGACTATGCAGCCTC
  ACTGCCGGAAGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGCGATGGACTGGTGGTAACT
  GTGGACAGTGAATCTGGGGATGTCCTGTGGATCCAAAACTATGCCTCTCCTGTGGTGGCCTTCTACGTCT
  GGCAGGGGGAGGTCCTGAGAAAGGTGGTGCACATCAACGTTGCTGTGGAGACTCTACGCTACTTGACCTT
  CATGTCTGGGGAAGTGGGGCGCATCACCAAGTGGAAGTATCCATTCCCCAAGGAGACAGAGGCCAAGAGC
  AAGCTAACGCCTACTCTGTATGTTGGGAAGTATTCCACCAGCCTCTATGCCTCTCCCTCAATGGTGCATG
  AGGGGGTTGCTGTCGTGCCTCGAGGCAGCACTCTTCCTTTGCTGGAAGGCCCCCAGACAGATGGCGTCAC
  CATTGGAGACAAAGGAGAGTGTGTGATCACTCCCAGCACAGACCTCAAGTTTGACCCTGGACTCAAAGGG
  AAGAGCAAGCTGAACTACTTGAGGAATTACTGGCTTCTCATAGGACACCATGAAACTCCTCTGTCTGCAT
  CCACCAAGATGCTGGAGAGATTTCCTAACAACCTGCCCAAACATCGAGAAAATGTGATTCCTGCTGATTC
  AGAAAAAAGGAGCTTTGAGGAAGTTATCAACATAGTTGGCCAGACTTCAGACAACACACCGACCACCGTA
  TCTCAGGATGTGGAGGAGAAGCTCGCTCGCGCCCCTGCCAAGCCTGAGGCCCCCGTGGACTCCATGCTCA
  AGGACATGGCTACCATTATCCTGAGCACCTTCCTGCTGGTTGGATGGGTGGCGTTCATCATCACTTACCC
  CCTGAGCGTGCATCAGCAGCGTCAGCTCCAGCACCAACAGTTCCAGAAGGAGCTGGAGAAGATTCAGCTC
  CTGCAGCAGCAGCAGCTGCCCTTCCACCCACACGGAGACCTTACCCAGGACCCTGAGTTCCTGGATTCAT
  CTGGCCCCTTCTCAGAGAGCTCTGGCACCAGCAGCCCCAGCCCATCCCCCAGAGCCTCCAACCACTCCCT
  CCACCCCAGCAGCTCTGCCTCCAGGGCCGGCACCAGCCCCTCTCTGGAGCAGGATGATGAGGATGAGGAA
  ACCAGAATGGTGATTGTTGGGAAAATTTCATTCTGCCCCAAGGATGTCCTGGGTCATGGAGCTGAGGGCA
  CAATTGTATACAAAGGTATGTTTGACAACCGAGATGTGGCCGTGAAGAGGATCCTCCCTGAGTGTTTTAG
  CTTTGCCGACCGTGAGGTCCAGCTGCTTCGAGAATCAGACGAGCACCCAAATGTGATCCGCTACTTTTGC
  ACAGAGAAGGACCGGCAGTTCCAGTACATTGCTATCGAGCTGTGTGCAGCCACCCTACAAGAGTATGTGG
  AGCAGAAGGACTTTGCCCACCTTGGCCTCGAGCCCATCACCCTGCTTCATCAGACCACCTCAGGCCTGGC
  ACACCTGCATTCTCTCAACATTGTTCACAGAGACCTGAAGCCCCACAACATTCTCCTCTCCATGCCCAAC
  GCACATGGCAGGATCAAGGCGATGATCTCTGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGGCACA
  GTTTCAGCCGCCGTTCAGGGGTACCTGGCACTGAAGGGTGGATCGCCCCAGAGATGCTGAGTGAAGACTG
  TAAGGACAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGTGTCTTTTACTATGTCATCTCTGAG
  GGCAACCATCCTTTTGGCAAATCCTTGCAGCGGCAGGCCAACATCCTCCTGGGCGCCTGCAACCTTGACT
  GTTTCCACTCAGACAAGCATGAGGACGTCATTGCTCGTGAATTGATAGAGAAAATGATTGCTATGGATCC
  CCAGCAGCGTCCCTCTGCAAAGCACGTGCTGAAACACCCCTTCTTCTGGAGCCTGGAGAAGCAGCTCCAG
  TTTTTCCAGGATGTAAGTGACCGAATAGAAAAGGAGGCCTTGGACGGTCCAATCGTACGGCAGTTGGAGA
  GAGGCGGGAGAGCTGTGGTCAAGATGGACTGGCGGGAGAACATCACTGTCCCCCTGCAGACAGATCTGCG
  CAAATTCAGAACCTACAAAGGTGGCTCTGTGAGAGACCTCCTCCGAGCCATGAGAAACAAGAAACACCAC
  TACCGGGAGCTCCCCGTGGAGGTTCAGGAGACGCTGGGCTCCATCCCGGATGACTTTGTGCGCTACTTCA
  CTTCCCGCTTCCCCCACCTCCTCTCTCACACCTACCAAGCCATGGAGCTGTGCAGACATGAGAGACTCTT
  TCAGACCTACTACTGGCACGAGCCCACAGAACCCCAGCCTCCAGTGATTCCATATGCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226999 representing NM_023913
  Red=Cloning site Green=Tags(s)
  
  MPARWLLLLLALLLPPPGPGSFGRTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHV
  EEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQT
  LSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVT
  VDSESGDVLWIQNYASPVVAFYVWQGEVLRKVVHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKS
  KLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFDPGLKG
  KSKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKRSFEEVINIVGQTSDNTPTTV
  SQDVEEKLARAPAKPEAPVDSMLKDMATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQFQKELEKIQL
  LQQQQLPFHPHGDLTQDPEFLDSSGPFSESSGTSSPSPSPRASNHSLHPSSSASRAGTSPSLEQDDEDEE
  TRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFC
  TEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPN
  AHGRIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVISE
  GNHPFGKSLQRQANILLGACNLDCFHSDKHEDVIARELIEKMIAMDPQQRPSAKHVLKHPFFWSLEKQLQ
  FFQDVSDRIEKEALDGPIVRQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHH
  YRELPVEVQETLGSIPDDFVRYFTSRFPHLLSHTYQAMELCRHERLFQTYYWHEPTEPQPPVIPYAL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023913
• ORF Size: 2931 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_023913.2, NP_076402.1
• RefSeq Size: 3976
• RefSeq ORF: 2934
• Locus ID: 78943