Stat4 (NM_011487) Mouse Tagged ORF Clone

CAT#: MG227035

Stat4 (GFP-tagged) - Mouse signal transducer and activator of transcription 4 (Stat4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Stat4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227035 representing NM_011487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTGGAATCAAGTCCAACAATTAGAAATCAAGTTTTTGGAGCAAGTAGATCAGTTCTATGATG
  ACAACTTTCCTATGGAAATCCGGCATCTGCTAGCTCAGTGGATTGAGACTCAAGACTGGGAAGTAGCTTC
  TAACAATGAAACTATGGCAACAATTCTGCTTCAAAACTTACTAATACAATTGGATGAACAGTCGGGGCGT
  GTTTCCAAAGAAAAAAATCTGCTATTGATTCACAATCTAAAGAGAATTAGAAAAGTTCTTCAGGGCAAGT
  TTCATGGAAATCCAATGCATGTAGCTGTGGTGATTTCAAATTGCTTAAGGGAAGAGAGGAGAATATTGGC
  TGCAGCCAACATGCCTATCCAGGGACCTCTGGAGAAATCCTTACAGAGTTCTTCAGTTTCTGAAAGACAA
  AGGAATGTGGAACACAAAGTGTCTGCCATTAAAAACAGTGTGCAGATGACAGAACAAGATACCAAATACT
  TAGAAGACCTGCAAGATGAGTTTGACTACAGGTATAAAACAATTCAGACAATGGATCAGGGTGACAAAAA
  CAGTATCCTGGTGAACCAGGAAGTTTTGACGCTGCAAGAAATGCTTAATAGTCTGGACTTCAAGAGAAAG
  GAAGCACTCAGTAAGATGACGCAGATAGTGAACGAGACAGACCTGCTCATGAACAGCATGCTTCTAGAAG
  AGCTGCAGGACTGGAAAAAGCGGCAGCAGATTGCCTGCATTGGTGGCCCGCTCCACAATGGGCTGGACCA
  GCTTCAGAACTGCTTTACCCTACTGGCAGAGAGTCTTTTCCAACTCAGACAGCAACTGGAGAAACTACAG
  GAGCAATCTACTAAAATGACCTATGAAGGGGATCCCATCCCTGCTCAAAGAGCACACCTCCTGGAAAGAG
  CTACCTTCCTGATCTACAACCTTTTCAAGAACTCATTTGTGGTCGAGCGACAGCCATGCATGCCAACGCA
  CCCTCAGAGGCCGATGGTACTTAAAACCCTCATTCAGTTCACTGTAAAACTGAGATTACTAATAAAATTG
  CCGGAACTAAACTATCAGGTGAAAGTAAAGGCGTCCATTGACAAGAATGTTTCAACTCTAAGCAATAGGA
  GATTTGTGCTTTGTGGAACTCACGTCAAAGCTATGTCCAGTGAGGAATCTTCCAATGGGAGCCTCTCAGT
  GGAGTTTAGACATTTGCAACCCAAGGAGATGAAGTGCAGTACTGGGAGTAAAGGAAACGAGGGCTGCCAC
  ATGGTGACAGAGGAGTTGCATTCCATAACCTTTGAGACCCAGATCTGCCTCTATGGCCTCACCATTAACC
  TAGAGACCAGCTCATTACCTGTCGTGATGATTTCTAATGTCAGCCAACTACCCAATGCATGGGCATCCAT
  CATTTGGTACAATGTATCAACTAACGACTCCCAGAACTTGGTTTTCTTTAATAACCCTCCATCTGTCACT
  TTGGGCCAACTCCTGGAAGTGATGAGCTGGCAATTTTCATCCTATGTCGGTCGTGGCCTTAATTCAGAGC
  AGCTCAACATGCTGGCAGAGAAGCTCACAGTTCAGTCTAACTACAATGATGGTCACCTCACCTGGGCCAA
  GTTCTGCAAGGAACATTTGCCTGGCAAAACATTTACCTTCTGGACTTGGCTTGAAGCAATATTGGACCTA
  ATTAAAAAACATATTCTTCCCCTCTGGATTGATGGGTACATCATGGGATTTGTTAGTAAAGAGAAGGAAC
  GGCTTCTGCTCAAAGATAAAATGCCTGGGACATTTTTGTTAAGATTCAGTGAGAGCCATCTTGGAGGGAT
  AACCTTCACCTGGGTGGACCAATCTGAAAATGGAGAAGTGAGATTCCACTCTGTAGAACCCTACAACAAA
  GGGAGACTGTCGGCTCTGCCGTTCGCTGACATCCTGCGAGACTACAAGGTTATCATGGCTGAAAACTTCC
  CTGAAAACCCTCTGAAGTACCTCTACCCTGACATTCCCAAAGACAAAGCCTTTGGCAAACACTACAGCTC
  CCAGCCGTGCGAAGTCTCAAGACCAACCGAACGGGGAGACAAGGGTTACGTCCCCTCTGTTTTTATCCCC
  ATTTCAACAATCCGAAGCGATTCCACGGAGCCACAATCTCCTTCAGACCTTCTCCCCATGTCTCCAAGTG
  CATATGCTGTGCTGAGAGAAAACCTGAGCCCAACGACAATTGAAACTGCAATGAATTCCCCATATTCTGC
  TGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227035 representing NM_011487
  Red=Cloning site Green=Tags(s)
  
  MAQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIETQDWEVASNNETMATILLQNLLIQLDEQSGR
  VSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPIQGPLEKSLQSSSVSERQ
  RNVEHKVSAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQGDKNSILVNQEVLTLQEMLNSLDFKRK
  EALSKMTQIVNETDLLMNSMLLEELQDWKKRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRQQLEKLQ
  EQSTKMTYEGDPIPAQRAHLLERATFLIYNLFKNSFVVERQPCMPTHPQRPMVLKTLIQFTVKLRLLIKL
  PELNYQVKVKASIDKNVSTLSNRRFVLCGTHVKAMSSEESSNGSLSVEFRHLQPKEMKCSTGSKGNEGCH
  MVTEELHSITFETQICLYGLTINLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPSVT
  LGQLLEVMSWQFSSYVGRGLNSEQLNMLAEKLTVQSNYNDGHLTWAKFCKEHLPGKTFTFWTWLEAILDL
  IKKHILPLWIDGYIMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDQSENGEVRFHSVEPYNK
  GRLSALPFADILRDYKVIMAENFPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIP
  ISTIRSDSTEPQSPSDLLPMSPSAYAVLRENLSPTTIETAMNSPYSAE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011487
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011487.4, NP_035617.1
• RefSeq Size: 2981
• RefSeq ORF: 2247
• Locus ID: 20849
• Gene Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Homozygous knockout mice for this gene exhibit reduced inflammation and cytokine production in response to immune challenge. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]