Best1 (NM_011913) Mouse Tagged ORF Clone

CAT#: MG227104

Best1 (GFP-tagged) - Mouse bestrophin 1 (Best1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Best1
• Synonyms: Bmd; mBest1; Vmd2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227104 representing NM_011913
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTATCACCTACACAAACAAAGTAGCCAATGCCCGCCTCGGTTCGTTCTCGTCCCTCCTCCTGTGCT
  GGCGAGGCAGCATCTACAAGCTGCTGTATGGAGAATTCCTTGTCTTCATATTCCTCTACTATTCCATCCG
  TGGACTCTACAGAATGGTTCTCTCGAGTGATCAGCAGCTGTTGTTTGAGAAGCTGGCTCTGTACTGCGAC
  AGCTACATTCAGCTCATCCCTATATCCTTCGTTCTGGGTTTCTATGTTACATTGGTGGTGAGCCGCTGGT
  GGAGCCAGTACGAGAACTTGCCGTGGCCCGACCGCCTCATGATCCAGGTGTCTAGCTTCGTGGAGGGCAA
  GGATGAGGAAGGCCGTTTGCTGCGGCGCACGCTCATCCGCTACGCCATCCTGGGCCAAGTGCTCATCCTG
  CGCAGCATCAGCACCTCGGTCTACAAGCGCTTTCCCACTCTTCACCACCTGGTGCTAGCAGGTTTTATGA
  CCCATGGGGAACATAAGCAGTTGCAGAAGTTGGGCCTACCACACAACACATTCTGGGTGCCCTGGGTGTG
  GTTTGCCAACTTGTCAATGAAGGCCTATCTTGGAGGTCGAATCCGGGACACCGTCCTGCTCCAGAGCCTG
  ATGAATGAGGTGTGTACTTTGCGTACTCAGTGTGGACAGCTGTATGCCTACGACTGGATAAGTATCCCAT
  TGGTGTACACACAGGTGGTGACAGTGGCAGTATACAGCTTTTTCCTTGCATGCTTGATCGGGAGGCAGTT
  TCTGAACCCAAACAAGGACTACCCAGGCCATGAGATGGATCTGGTTGTGCCTGTCTTCACAATCCTGCAA
  TTCTTATTCTACATGGGCTGGCTGAAGGTGGCAGAACAGCTCATCAACCCCTTCGGGGAGGACGATGATG
  ATTTTGAGACTAACTGGATCATTGACAGAAACCTGCAGGTGTCCCTGTTGTCCGTGGATGGGATGCACCA
  GAACTTGCCTCCCATGGAACGTGACATGTACTGGAACGAGGCAGCGCCTCAGCCGCCCTACACAGCTGCT
  TCTGCCAGGTCTCGCCGGCATTCCTTCATGGGCTCCACCTTCAACATCAGCCTAAAGAAAGAAGACTTAG
  AGCTTTGGTCAAAAGAGGAGGCTGACACGGATAAGAAAGAGAGTGGCTATAGCAGCACCATAGGCTGCTT
  CTTAGGACTGCAACCCAAAAACTACCATCTTCCCTTGAAAGACTTAAAGACCAAACTATTGTGTTCTAAG
  AACCCCCTCCTCGAAGGCCAGTGTAAGGATGCCAACCAGAAAAACCAGAAAGATGTCTGGAAATTTAAGG
  GTCTGGACTTCTTGAAATGTGTTCCAAGGTTTAAGAGGAGAGGCTCCCATTGTGGCCCACAGGCACCCAG
  CAGCCACCCTACTGAGCAGTCAGCACCCTCCAGTTCAGACACAGGTGATGGGCCTTCCACAGATTACCAA
  GAAATCTGTCACATGAAAAAGAAAACTGTGGAGTTTAACTTGAACATTCCAGAGAGCCCCACAGAACATC
  TTCAACAGCGCCGTTTGGACCAGATGTCAACCAATATACAGGCTCTAATGAAGGAGCATGCAGAGTCCTA
  TCCCTACAGGGATGAAGCTGGCACCAAACCTGTTCTCTATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227104 representing NM_011913
  Red=Cloning site Green=Tags(s)
  
  MTITYTNKVANARLGSFSSLLLCWRGSIYKLLYGEFLVFIFLYYSIRGLYRMVLSSDQQLLFEKLALYCD
  SYIQLIPISFVLGFYVTLVVSRWWSQYENLPWPDRLMIQVSSFVEGKDEEGRLLRRTLIRYAILGQVLIL
  RSISTSVYKRFPTLHHLVLAGFMTHGEHKQLQKLGLPHNTFWVPWVWFANLSMKAYLGGRIRDTVLLQSL
  MNEVCTLRTQCGQLYAYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLNPNKDYPGHEMDLVVPVFTILQ
  FLFYMGWLKVAEQLINPFGEDDDDFETNWIIDRNLQVSLLSVDGMHQNLPPMERDMYWNEAAPQPPYTAA
  SARSRRHSFMGSTFNISLKKEDLELWSKEEADTDKKESGYSSTIGCFLGLQPKNYHLPLKDLKTKLLCSK
  NPLLEGQCKDANQKNQKDVWKFKGLDFLKCVPRFKRRGSHCGPQAPSSHPTEQSAPSSSDTGDGPSTDYQ
  EICHMKKKTVEFNLNIPESPTEHLQQRRLDQMSTNIQALMKEHAESYPYRDEAGTKPVLYE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011913
• ORF Size: 1653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011913.2, NP_036043.2
• RefSeq Size: 2087
• RefSeq ORF: 1656
• Locus ID: 24115