Nefh (NM_010904) Mouse Tagged ORF Clone

CAT#: MG227134

Nefh (GFP-tagged) - Mouse neurofilament heavy polypeptide (Nefh), (10ug). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nefh
• Synonyms: mKIAA0845; NF-H; NF200; Nfh
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227134 representing NM_010904, codon optimized.
  Due to the complexity of NM_010904, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCCTTCGGATCAGCAGACGCGCTTCTCGGCGCCCCTTTTGCTCCTCTTCATGGCGGCGGTTCTC
  TTCATTACAGCCTGAGCCGCAAAGCAGGGCCAGGCGGGACCCGGAGCGCTGCTGGCTCCAGTAGCGGTTT
  CCACTCATGGGCCCGCACCAGCGTCAGTTCTGTCAGTGCTAGTCCAAGCAGGTTTCGGGGAGCAGCCTCT
  TCTACGGACTCTCTGGATACCCTTAGCAACGGGCCCGAGGGATGTGTCGTTGCAGCCGTCGCTGCGCGCA
  GCGAGAAGGAGCAGCTGCAGGCTCTGAATGATCGCTTTGCTGGATACATTGATAAAGTTCGGCAACTGGA
  AGCTCATAATAGATCTCTCGAAGGTGAGGCAGCCGCCCTCAGGCAGCAGCAGGCCGGACGAGCCGCTATG
  GGAGAACTCTACGAAAGAGAGGTCCGGGAAATGAGAGGGGCCGTGCTCAGACTGGGCGCTGCTCGAGGGC
  AATTGAGGCTCGAGCAGGAACACTTGCTCGAGGATATCGCCCACGTCCGGCAGAGACTGGACGAGGAGGC
  TAGACAGCGCGAAGAGGCCGAAGCTGCCGCCAGAGCCTTGGCACGCTTCGCCCAAGAGGCTGAGGCTGCT
  CGCGTTGAACTGCAGAAGAAAGCCCAGGCACTGCAGGAAGAATGTGGATACCTGCGCCGGCACCATCAGG
  AAGAAGTCGGGGAGTTGCTGGGACAGATTCAAGGCTGCGGCGCCGCCCAGGCACAAGCCCAGGCCGAAGC
  CCGGGACGCTCTGAAATGTGATGTCACGAGCGCACTCCGCGAGATACGAGCCCAGCTGGAGGGGCACGCC
  GTGCAGTCAACACTCCAGTCAGAGGAATGGTTCAGAGTCCGGCTGGATCGACTGTCCGAGGCCGCAAAGG
  TGAATACAGACGCCATGCGCTCTGCACAGGAAGAGATAACAGAGTACAGACGGCAGCTGCAGGCTAGGAC
  TACCGAGCTCGAAGCACTCAAGAGCACCAAGGAGAGTTTGGAGAGACAACGGAGCGAGCTGGAGGATAGG
  CACCAGGCTGACATTGCTTCATACCAAGACGCGATCCAACAGTTGGATTCCGAACTGCGCAATACCAAAT
  GGGAGATGGCCGCTCAGCTCAGAGAATACCAAGACCTGTTGAATGTCAAGATGGCTCTGGATATTGAGAT
  TGCTGCTTACCGCAAACTGCTGGAAGGCGAAGAGTGCCGGATAGGCTTTGGCCCCTCACCATTTTCTCTG
  ACAGAAGGACTTCCCAAGATCCCCAGCATCAGTACTCATATCAAAGTTAAAAGTGAGGAAATGATCAAGG
  TGGTAGAGAAGAGCGAAAAAGAAACGGTCATAGTGGAGGGTCAAACTGAAGAAATCAGGGTGACCGAGGG
  CGTGACCGAGGAAGAAGATAAAGAAGCGCAAGGGCAAGAGGGGGAGGAAGCCGAGGAAGGTGAAGAAAAG
  GAGGAAGAAGAAGGGGCTGCTGCCACATCCCCGCCAGCAGAAGAGGCCGCCAGCCCAGAGAAAGAGACTA
  AGAGCCGGGTGAAGGAGGAAGCCAAATCACCTGGCGAGGCCAAATCTCCCGGAGAGGCCAAAAGCCCAGC
  TGAAGCAAAGTCCCCAGGGGAGGCAAAGTCCCCAGGTGAGGCCAAGTCTCCCGGAGAGGCCAAGTCTCCA
  GCTGAGCCAAAGTCACCAGCGGAGCCAAAAAGTCCTGCTGAAGCAAAGAGCCCCGCAGAGCCTAAATCTC
  CCGCAACCGTCAAAAGCCCAGGCGAGGCTAAATCCCCTAGTGAGGCCAAATCTCCCGCTGAAGCCAAGAG
  CCCCGCCGAGGCTAAAAGCCCCGCAGAAGCCAAGAGTCCTGCCGAAGCCAAATCTCCAGCGGAGGCCAAA
  TCACCTGCGGAGGCAAAAAGTCCAGCGACCGTGAAGTCTCCGGGCGAGGCTAAGTCACCATCCGAAGCTA
  AGTCTCCGGCCGAGGCTAAATCCCCAGCAGAAGCAAAAAGTCCTGCGGAGGCTAAAAGCCCCGCTGAGGT
  GAAAAGTCCTGGCGAGGCCAAATCTCCTGCTGAGCCTAAGTCTCCTGCAGAGGCAAAATCCCCGGCTGAG
  GTCAAAAGCCCCGCGGAAGCTAAGTCCCCCGCTGAAGTAAAATCCCCTGGAGAAGCCAAGAGCCCCGCTG
  CCGTCAAGTCACCCGCGGAAGCTAAGTCTCCCGCTGCTGTCAAATCACCCGGGGAGGCTAAGTCACCTGG
  AGAGGCTAAGTCACCCGCAGAGGCAAAAAGCCCTGCCGAAGCTAAGTCCCCCATCGAAGTAAAGAGCCCT
  GAAAAGGCAAAGACCCCGGTGAAAGAGGGTGCCAAGTCCCCCGCCGAGGCAAAGTCCCCCGAGAAGGCAA
  AATCTCCTGTGAAGGAAGACATCAAGCCCCCCGCAGAGGCCAAAAGTCCAGAGAAGGCTAAGTCTCCCGT
  GAAAGAAGGGGCTAAACCACCAGAGAAGGCAAAGCCTCTGGACGTGAAGAGCCCAGAGGCACAGACACCA
  GTCCAGGAAGAGGCCAAACACCCTACGGACATTCGGCCGCCAGAACAGGTGAAGTCACCCGCAAAAGAGA
  AGGCCAAGAGTCCTGAGAAGGAAGAGGCTAAGACTTCCGAGAAGGTGGCTCCTAAAAAAGAGGAAGTAAA
  AAGTCCCGTCAAAGAAGAGGTGAAAGCTAAAGAGCCACCGAAGAAAGTCGAAGAAGAGAAAACTTTGCCT
  ACTCCCAAGACAGAGGCCAAGGAATCTAAAAAGGACGAAGCTCCTAAGGAGGCTCCAAAGCCCAAGGTCG
  AGGAAAAAAAGGAGACTCCAACAGAGAAGCCTAAAGACAGCACCGCCGAAGCCAAAAAGGAGGAGGCGGG
  CGAGAAGAAAAAGGCTGTTGCGAGCGAGGAGGAGACACCAGCAAAGCTGGGAGTGAAGGAAGAGGCCAAG
  CCCAAAGAAAAGACCGAAACAACAAAGACGGAGGCCGAGGACACTAAGGCTAAAGAACCAAGTAAACCCA
  CCGAAACTGAAAAGCCCAAAAAAGAGGAGATGCCCGCTGCTCCTGAAAAGAAGGATACCAAGGAGGAAAA
  AACTACCGAGAGCAGGAAGCCGGAGGAAAAGCCTAAGATGGAGGCCAAGGTCAAGGAAGACGATAAGTCC
  CTGTCCAAGGAACCGAGCAAGCCCAAAACGGAGAAGGCAGAAAAGAGCTCTTCTACAGATCAAAAAGAGT
  CCCAACCCCCAGAGAAAACCACAGAGGATAAGGCCACGAAGGGCGAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227134 representing NM_010904
  Red=Cloning site Green=Tags(s)
  
  MMSFGSADALLGAPFAPLHGGGSLHYSLSRKAGPGGTRSAAGSSSGFHSWARTSVSSVSASPSRFRGAAS
  STDSLDTLSNGPEGCVVAAVAARSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAM
  GELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDEEARQREEAEAAARALARFAQEAEAA
  RVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHA
  VQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKESLERQRSELEDR
  HQADIASYQDAIQQLDSELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFSL
  TEGLPKIPSISTHIKVKSEEMIKVVEKSEKETVIVEGQTEEIRVTEGVTEEEDKEAQGQEGEEAEEGEEK
  EEEEGAAATSPPAEEAASPEKETKSRVKEEAKSPGEAKSPGEAKSPAEAKSPGEAKSPGEAKSPGEAKSP
  AEPKSPAEPKSPAEAKSPAEPKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEAKSPAEAK
  SPAEAKSPATVKSPGEAKSPSEAKSPAEAKSPAEAKSPAEAKSPAEVKSPGEAKSPAEPKSPAEAKSPAE
  VKSPAEAKSPAEVKSPGEAKSPAAVKSPAEAKSPAAVKSPGEAKSPGEAKSPAEAKSPAEAKSPIEVKSP
  EKAKTPVKEGAKSPAEAKSPEKAKSPVKEDIKPPAEAKSPEKAKSPVKEGAKPPEKAKPLDVKSPEAQTP
  VQEEAKHPTDIRPPEQVKSPAKEKAKSPEKEEAKTSEKVAPKKEEVKSPVKEEVKAKEPPKKVEEEKTLP
  TPKTEAKESKKDEAPKEAPKPKVEEKKETPTEKPKDSTAEAKKEEAGEKKKAVASEEETPAKLGVKEEAK
  PKEKTETTKTEAEDTKAKEPSKPTETEKPKKEEMPAAPEKKDTKEEKTTESRKPEEKPKMEAKVKEDDKS
  LSKEPSKPKTEKAEKSSSTDQKESQPPEKTTEDKATKGEK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010904
• ORF Size: 3270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010904.3, NP_035034.2
• RefSeq Size: 4004
• RefSeq ORF: 3273
• Locus ID: 380684
• Gene Summary: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProtKB/Swiss-Prot Function]