Eno1 (NM_023119) Mouse Tagged ORF Clone

CAT#: MG227149

Eno1 (GFP-tagged) - Mouse enolase 1 alpha non-neuron (Eno1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eno1
• Synonyms: 0610008I15; AL022784; Eno-1; MBP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227149 representing NM_023119
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATTCTCAGGATCCACGCCAGAGAGATCTTTGACTCCCGTGGGAATCCCACTGTTGAGGTCGATC
  TGTACACCGCAAAAGGTCTCTTCCGAGCTGCGGTGCCCAGCGGTGCGTCCACTGGCATCTACGAGGCCCT
  AGAACTCCGAGACAATGATAAGACCCGCTTCATGGGGAAGGGTGTCTCACAGGCTGTTGAGCACATCAAT
  AAAACTATTGCGCCTGCTCTGGTTAGCAAGAAAGTGAATGTTGTGGAGCAAGAGAAGATTGACAAGCTGA
  TGATCGAGATGGACGGCACAGAGAATAAATCTAAATTTGGTGCAAATGCCATCCTGGGAGTGTCCCTGGC
  TGTCTGCAAAGCTGGTGCCGTGGAAAAGGGGGTGCCCCTTTACCGCCACATTGCTGACTTGGCCGGCAAC
  CCTGAAGTCATCCTGCCTGTCCCGGCTTTCAATGTGATCAACGGTGGTTCTCATGCTGGCAACAAGCTGG
  CCATGCAAGAGTTCATGATCCTGCCTGTGGGGGCATCCAGCTTCCGGGAAGCCATGCGCATTGGAGCAGA
  GGTTTACCACAACCTGAAGAACGTGATCAAGGAGAAGTACGGGAAGGACGCCACCAATGTGGGTGATGAG
  GGTGGATTCGCACCTAACATCCTGGAGAACAAAGAAGCACTGGAGCTGCTCAAGACTGCAATCGCAAAGG
  CCGGCTACACTGACCAGGTTGTCATTGGCATGGATGTGGCTGCCTCCGAGTTCTACAGGTCTGGCAAGTA
  TGACCTGGACTTCAAGTCTCCGGATGACCCCAGCAGGTACATCACTCCCGACCAGCTGGCTGATCTGTAC
  AAGTCCTTCGTCCAGAACTACCCAGTGGTGTCCATCGAAGATCCCTTTGACCAGGACGACTGGGGCGCCT
  GGCAGAAGTTCACGGCTAGTGCGGGCATCCAGGTGGTGGGCGATGACCTCACAGTGACCAACCCTAAGCG
  GATTGCCAAGGCTGCGAGCGAGAAGTCCTGCAACTGCCTCTTGCTCAAAGTGAACCAGATCGGCTCTGTG
  ACCGAATCCCTGCAGGCGTGTAAGCTGGCCCAATCCAATGGCTGGGGCGTCATGGTGTCCCACCGATCTG
  GGGAAACTGAGGACACTTTCATCGCAGACCTGGTGGTGGGGCTCTGCACTGGGCAGATCAAGACTGGTGC
  CCCTTGCCGATCCGAGCGCCTGGCCAAGTACAATCAGATCCTCAGAATTGAGGAAGAGCTGGGCAGCAAA
  GCCAAGTTTGCTGGCAGGTCCTTCAGGAACCCCCTGGCCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227149 representing NM_023119
  Red=Cloning site Green=Tags(s)
  
  MSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHIN
  KTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGN
  PEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDE
  GGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLY
  KSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSV
  TESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSK
  AKFAGRSFRNPLAK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023119
• ORF Size: 1302 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_023119.2, NP_075608.2
• RefSeq Size: 1831
• RefSeq ORF: 1305
• Locus ID: 13806