Trpv1 (NM_001001445) Mouse Tagged ORF Clone

CAT#: MG227160

Trpv1 (GFP-tagged) - Mouse transient receptor potential cation channel subfamily V member 1 (Trpv1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpv1
• Synonyms: OTRPC1; TRPV1alpha; TRPV1beta; VR-1; Vr1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227160 representing NM_001001445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAATGGGCTAGCTTAGACTCGGATGAATCTGAGCCCCCAGCCCAAGAGAACTCCTGCCCGGACC
  CTCCAGACAGAGACCCTAACTCCAAGCCGCCTCCAGCCAAGCCCCACATCTTTGCTACCAGGAGTCGCAC
  CCGGCTTTTTGGGAAGGGTGACTCAGAAGAGGCCTCTCCCATGGACTGCCCTTATGAGGAAGGCGGGCTG
  GCCTCCTGCCCTATCATCACCGTCAGCTCTGTTGTCACTCTCCAGAGGTCTGTGGATGGACCTACCTGTC
  TCAGGCAGACATCCCAGGACTCTGTCTCCACTGGTGTTGAGACGCCCCCAAGGCTCTATGATCGCAGGAG
  CATCTTCGACGCTGTGGCTCAGAGCAACTGCCAGGAGCTGGAGAGCCTGCTGTCCTTCCTGCAGAAGAGC
  AAGAAGCGCCTGACTGACAGCGAGTTCAAAGACCCAGAGACGGGAAAGACCTGTCTGCTCAAAGCCATGC
  TCAATCTGCACAATGGGCAGAACGACACCATTGCTCTGCTCCTGGACATTGCCCGGAAGACAGATAGCCT
  GAAGCAGTTTGTCAATGCCAGCTACACAGACAGCTACTACAAGGGCCAGACAGCATTACACATTGCCATT
  GAAAGGCGGAACATGGCACTGGTGACCCTCTTGGTGGAGAATGGAGCAGATGTCCAGGCTGCTGCTAACG
  GGGACTTCTTCAAGAAAACCAAAGGGAGGCCTGGCTTCTACTTTGGTGAGCTGCCCCTGTCCCTGGCTGC
  GTGCACCAACCAGCTGGCCATTGTGAAGTTCCTGCTGCAGAACTCCTGGCAGCCTGCAGACATCAGTGCA
  CGGGATTCGGTGGGCAACACGGTGCTGCACGCCCTTGTGGAGGTGGCAGATAACACAGCTGACAACACCA
  AGTTCGTGACAAACATGTACAACGAGATCCTGATCCTGGGGGCCAAACTCCACCCCACACTGAAGCTAGA
  AGAACTCACCAACAAGAAGGGGCTTACACCGCTGGCTCTGGCTGCCAGCAGTGGGAAGATTGGGGTCTTG
  GCCTACATTCTCCAGAGGGAGATCCACGAACCAGAGTGCCGGCACCTGTCCAGGAAGTTCACTGAATGGG
  CCTATGGGCCCGTGCACTCCTCCCTTTATGACCTGTCCTGCATTGACACCTGTGAGAAGAATTCAGTGCT
  GGAGGTGATCGCCTACAGTAGCAGTGAGACCCCCAACCGCCACGACATGCTTCTCGTGGAGCCCTTGAAC
  CGACTCCTGCAGGACAAGTGGGACAGATTTGTCAAGCGCATCTTCTACTTCAACTTCTTCGTCTACTGCT
  TGTATATGATCATCTTCACCACGGCTGCTTACTATCGGCCTGTGGAAGGCTTGCCCCCCTATAAGCTGAA
  TAACACCGTTGGGGACTATTTCCGTGTCACTGGAGAGATCCTGTCTGTGTCAGGAGGAGTCTACTTCTTC
  TTCCGAGGGATCCAGTATTTCCTGCAGAGGCGACCATCCCTCAAGAGTTTGTTTGTGGACAGCTACAGTG
  AGATACTTTTCTTTGTACAGTCACTGTTCATGCTGGTGTCTGTGGTACTGTACTTCAGCCATCGCAAGGA
  GTATGTGGCTTCCATGGTGTTCTCCCTGGCCATGGGCTGGACCAACATGCTCTACTACACCCGAGGATTC
  CAGCAGATGGGCATCTATGCTGTCATGATTGAGAAGATGATCCTCAGAGACCTGTGTCGGTTTATGTTCG
  TCTACCTCGTGTTCTTGTTTGGATTTTCCACAGCCGTAGTGACACTGATCGAGGATGGGAAGAATAACTC
  ACTGCCTGTGGAGTCCCCACCACACAAGTGTCGGGGATCTGCCTGCAGGCCAGGTAACTCTTACAACAGC
  CTGTATTCCACATGTCTGGAGCTGTTCAAGTTCACCATCGGCATGGGTGACCTGGAGTTCACCGAGAACT
  ATGACTTCAAGGCTGTCTTCATCATCCTGTTACTGGCCTATGTGATTCTCACCTACATCCTCCTGCTCAA
  CATGCTCATTGCTCTCATGGGCGAGACTGTCAACAAGATTGCACAAGAGAGCAAGAACATCTGGAAGCTG
  CAGCGAGCCATCACCATCCTGGATACAGAGAAGAGTTTCCTGAAGTGCATGAGGAAGGCCTTCCGCTCCG
  GCAAGCTGCTGCAGGTGGGGTTCACGCCGGACGGCAAGGATGACTTCCGGTGGTGCTTCAGGGTGGATGA
  GGTGAACTGGACTACCTGGAACACCAACGTGGGCATCATCAACGAGGACCCAGGCAACTGTGAGGGCGTC
  AAGCGCACCCTGAGCTTCTCCCTGCGGTCAGGCCGAGTTTCAGGGAGAAACTGGAAGAACTTTGCCCTGG
  TTCCCCTTCTGAGGGACGCAAGCACTCGAGATAGGCATAGCACCCAGCCGGAAGAAGTTCAGCTGAAGCA
  CTATACGGGATCCCTTAAGCCAGAGGATGCTGAGGTCTTCAAGGATTCCATGGCCCCAGGGGAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227160 representing NM_001001445
  Red=Cloning site Green=Tags(s)
  
  MEKWASLDSDESEPPAQENSCPDPPDRDPNSKPPPAKPHIFATRSRTRLFGKGDSEEASPMDCPYEEGGL
  ASCPIITVSSVVTLQRSVDGPTCLRQTSQDSVSTGVETPPRLYDRRSIFDAVAQSNCQELESLLSFLQKS
  KKRLTDSEFKDPETGKTCLLKAMLNLHNGQNDTIALLLDIARKTDSLKQFVNASYTDSYYKGQTALHIAI
  ERRNMALVTLLVENGADVQAAANGDFFKKTKGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADISA
  RDSVGNTVLHALVEVADNTADNTKFVTNMYNEILILGAKLHPTLKLEELTNKKGLTPLALAASSGKIGVL
  AYILQREIHEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN
  RLLQDKWDRFVKRIFYFNFFVYCLYMIIFTTAAYYRPVEGLPPYKLNNTVGDYFRVTGEILSVSGGVYFF
  FRGIQYFLQRRPSLKSLFVDSYSEILFFVQSLFMLVSVVLYFSHRKEYVASMVFSLAMGWTNMLYYTRGF
  QQMGIYAVMIEKMILRDLCRFMFVYLVFLFGFSTAVVTLIEDGKNNSLPVESPPHKCRGSACRPGNSYNS
  LYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKL
  QRAITILDTEKSFLKCMRKAFRSGKLLQVGFTPDGKDDFRWCFRVDEVNWTTWNTNVGIINEDPGNCEGV
  KRTLSFSLRSGRVSGRNWKNFALVPLLRDASTRDRHSTQPEEVQLKHYTGSLKPEDAEVFKDSMAPGEK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001445
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001445.1, NP_001001445.1
• RefSeq Size: 2520 bp
• RefSeq ORF: 2520 bp
• Locus ID: 193034
• Cytogenetics: 11 45.25 cM