Ncam1 (NM_001081445) Mouse Tagged ORF Clone

CAT#: MG227176

Ncam1 (GFP-tagged) - Mouse neural cell adhesion molecule 1 (Ncam1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ncam1
• Synonyms: CD56; E-NCAM; Ncam; NCAM-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227176 representing NM_001081445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCCCTGCAGGTAGATA
  TTGTTCCCAGCCAAGGAGAAATCAGCGTTGGAGAGTCCAAATTCTTCCTGTGTCAAGTGGCAGGAGATGC
  CAAAGATAAGGACATCTCCTGGTTCTCCCCCAATGGGGAGAAGCTGAGCCCAAACCAGCAGCGGATCTCA
  GTGGTGTGGAATGATGACGACTCCTCTACCCTCACCATCTACAACGCCAACATCGACGATGCCGGCATAT
  ACAAGTGTGTGGTCACGGCTGAGGACGGCACCCAGTCTGAGGCCACTGTCAACGTGAAGATCTTCCAGAA
  GCTCATGTTCAAGAATGCACCAACCCCACAGGAGTTTAAGGAAGGGGAGGATGCTGTGATTGTCTGTGAT
  GTGGTCAGCTCCCTGCCTCCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATTCTGAAAAAAGACG
  TCCGGTTCATAGTCCTGTCCAACAACTACCTGCAGATCAGGGGCATCAAGAAAACAGATGAGGGTACTTA
  CCGCTGTGAGGGCAGGATCCTGGCCCGCGGGGAAATCAACTTCAAGGACATTCAGGTCATTGTGAATGTA
  CCACCCACTGTCCAGGCCAGACAGAGCATCGTGAATGCCACTGCCAACCTGGGCCAGTCTGTCACCCTGG
  TGTGTGATGCCGATGGCTTCCCAGAGCCCACCATGAGCTGGACAAAGGATGGGGAACCCATAGAGAACGA
  GGAGGAAGATGACGAGAAGCACATCTTCAGTGATGACAGCTCCGAGCTGACCATCAGGAATGTGGATAAA
  AACGACGAGGCCGAATACGTCTGCATCGCAGAGAACAAGGCTGGCGAGCAGGATGCCTCCATCCACCTCA
  AGGTCTTTGCAAAGCCCAAAATCACCTATGTAGAGAATCAGACGGCCATGGAACTAGAGGAGCAAGTCAC
  TCTGACCTGTGAAGCCTCCGGAGACCCCATCCCCTCCATCACCTGGAGAACATCCACCCGGAACATCAGC
  AGTGAAGAAAAGACTCTGGATGGGCACATGGTGGTTCGCAGCCATGCTCGTGTGTCCTCCTTGACCCTGA
  AGAGCATCCAGTACACAGATGCTGGAGAATACATCTGCACTGCCAGCAACACCATCGGCCAGGATTCCCA
  GTCCATGTACCTCGAAGTTCAATATGCTCCCAAGCTCCAGGGCCCTGTGGCTGTGTACACTTGGGAAGGG
  AACCAGGTGAACATCACCTGTGAGGTCTTCGCCTACCCAAGTGCCACAATCTCCTGGTTCCGAGATGGTC
  AGTTGCTGCCAAGCTCCAACTACAGCAATATCAAGATCTACAACACCCCATCTGCTAGCTATCTGGAGGT
  GACCCCTGATTCAGAAAATGACTTTGGAAACTACAACTGTACAGCAGTGAACCGTATTGGACAGGAGTCC
  TTGGAATTCATCCTTGTTCAAGCAGACACACCGTCTTCTCCATCCATTGACCGGGTGGAACCATACTCCA
  GCACAGCGCAGGTGCAGTTTGATGAGCCAGAGGCCACAGGGGGCGTGCCCATTCTCAAGTACAAGGCTGA
  GTGGAAGTCGCTGGGCGAAGAATCCTGGCATTTCAAGTGGTATGATGCCAAAGAAGCCAACATGGAAGGC
  ATTGTCACCATCATGGGCCTGAAACCTGAGACGAGGTACTCGGTACGACTGGCGGCCCTCAACGGCAAGG
  GGCTGGGCGAGATCAGTGCAGCCACTGAGTTCAAGACACAGCCAGTCCGGGAACCCAGTGCACCCAAGCT
  GGAAGGGCAGATGGGAGAGGACGGGAACTCCATCAAGGTGAACCTGATCAAGCAGGATGACGGAGGCTCC
  CCCATCAGACACTATCTGGTCAAGTACAGAGCGCTCGCCTCTGAGTGGAAACCGGAAATCAGGCTCCCAT
  CCGGCAGTGACCACGTCATGCTCAAGTCCCTGGACTGGAACGCAGAGTATGAAGTCTATGTGGTAGCTGA
  AAACCAGCAAGGAAAATCCAAGGCAGCTCACTTTGTGTTCAGGACCTCAGCCCAGCCCACGGCCATCCCA
  GCCAATGGCAGCCCCACCGCAGGCCTGAGCACAGGCGCCATTGTGGGCATCCTCATTGTCATCTTCGTCC
  TGCTCCTGGTGGTCATGGACATCACCTGCTACTTCCTGAACAAGTGTGGCCTGCTCATGTGCATCGCTGT
  TAACCTGTGTGGCAAAGCTGGGCCCGGAGCCAAGGGCAAAGACATGGAGGAGGGCAAGGCTGCTTTCTCG
  AAAGATGAGTCAAAAGAACCCATTGTGGAGGTCCGAACGGAGGAAGAACGGACTCCAAACCATGATGGGG
  GGAAGCACACAGAGCCCAACGAGACCACGCCGCTGACAGAACCCGAAAAGGGCCCTGTAGAAACAAAGTC
  TGAGCCCCCGGAGTCAGAAGCCAAGCCAGCGCCAACTGAAGTCAAGACGGTCCCCAACGATGCCACACAA
  ACAAAAGAGAATGAGAGCAAAGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227176 representing NM_001081445
  Red=Cloning site Green=Tags(s)
  
  MLRTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRIS
  VVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCD
  VVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNV
  PPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDK
  NDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNIS
  SEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEG
  NQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQES
  LEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEG
  IVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGS
  PIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIP
  ANGSPTAGLSTGAIVGILIVIFVLLLVVMDITCYFLNKCGLLMCIAVNLCGKAGPGAKGKDMEEGKAAFS
  KDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVETKSEPPESEAKPAPTEVKTVPNDATQ
  TKENESKA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081445
• ORF Size: 2544 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081445.1, NP_001074914.1
• RefSeq Size: 6192
• RefSeq ORF: 2547
• Locus ID: 17967
• Gene Summary: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. [UniProtKB/Swiss-Prot Function]