Irak1 (NM_008363) Mouse Tagged ORF Clone

CAT#: MG227189

Irak1 (GFP-tagged) - Mouse interleukin-1 receptor-associated kinase 1 (Irak1) transcript variant 4, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Irak1
• Synonyms: AA408924; Il1rak; IRAK; IRAK-1; IRAK1-S; IRAK1b; mPLK; Plpk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227189 representing NM_008363
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGGGCCGGGCCCCGGGGAGCCTGTGGTTCCAGGCGCCCAGCACTTCTTGTACGAGGTGCCAC
  CCTGGGTTATGTGCCGCTTCTACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCGGC
  CTTGATCGTGCGCGACCAGACAGAGCTGCGGCTGTGCGAGCGCTCCGAGCAGCGCACAGCCAGTGTTCTG
  TGGCCCTGGATCAACCGCAACGCGCGCGTAGCTGACCTCGTTCACATCCTCACGCACCTGCAGCTGCTGC
  GTGCGAGGGACATCATCACAGCCTGGCACCCTCCTGCCCCCGTTGTGCCCCCAAGCACCGCTGCCCCAAG
  GCCCAGCAGCATCTCTGCAGGCTCTGAGGCCGGGGACTGGAGCCCCCGGAAATTGCAGTCCTCTGCCTCC
  ACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTCTGAGTCAGAGCTCCTCCAGGTTCCACTCC
  CTGTTTCCCTCGGGCCACCACTACCATCTTCAGCCCCTTCCTCCACCAAGTCAAGCCCAGAGAGCCCAGT
  GTCTGGCCTCCAAAGAGCCCATCCCTCCCCGTTTTGCTGGCCCTTCTGTGAGATTTCCCAAGGCACCTGC
  AACTTCTCTGAAGAACTCAGGATTGGAGAGGGTGGTTTTGGATGTGTGTACCGAGCAGTCATGAGAAATA
  CTACATATGCTGTGAAGAGACTGAAGGAGGAAGCTGACCTAGAGTGGACTATGGTGAAACAGAGCTTCTT
  AACAGAGGTGGAACAGCTATCAAGGTTTCGTCACCCAAATATCGTAGACTTTGCTGGCTACTGTGCAGAG
  AGTGGCTTATACTGCCTTGTTTATGGCTTCTTGCCCAATGGCTCCTTGGAGGATCAGCTCCACCTTCAGA
  CCCAGGCCTGCTCCCCACTTTCCTGGCCTCAACGACTGGACATTCTTCTGGGCACAGCCCGGGCTATTCA
  GTTTTTACATCAGGATAGCCCCAGCCTTATCCATGGAGACATCAAGAGTTCTAACGTGCTTCTGGATGAG
  AGACTGATGCCCAAGCTGGGAGACTTTGGCCTGGCTCGTTTCAGCCGCTTTGCGGGGGCCAAAGCAAGCC
  AGAGCAGTACTGTGGCCCGGACTTCCACAGTTCGAGGTACCCTCGCCTACTTGCCTGAGGAGTACATCAA
  GACAGGCCGACTGGCTGTGGACACCGATACCTTCAGCTTTGGGGTGGTAATACTGGAGACCCTTGCTGGT
  CAGAGGGCTGTGAGGACACAAGGTGCAAAGACCAAGTATTTGAAAGACCTGATTGAAGATGAGGCTGAAG
  AGGCTGGAGTGACCCTGAAAAGCACCCAGCCTACTCTGTGGGTGGGTGTAGCCACGGATGCTTGGGCTGC
  TCCAATTGCTGCCCAGATCTATAAGAAGCACCTGGACTCCAGACCTGGGCCCTGCCCACCCCAGTTGGGC
  CTGGCCCTGGCTCAACTAGCTTGCTGCTGCATGCACCGTCGGGCCAAGAAGAGGCCCCCCATGACCCAGG
  TATACAAGAGACTAGAAGGGCTTCAGGCAGGGCCTCCCTGGGAGCTAGAGGTTGCCGGCCATGGCTCCCC
  TTCCCCACAGGAGAACTCCTACATGTCTACCACTGGCAGTGCCCAGAGTGGGGATGAACCATGGCAGCCT
  CTAGTAGTGACCACAAGAGCCCCAGCCCAGGCTGCCCAGCAACTCCAGAGAAGTCCCAACCAGCCAGTGG
  AAAGTGATGAGAGTGTTCCCGGCCTCTCTGCTACCCTGCATTCCTGGCACTTGACTCCAGGTTCCCACCC
  AAGCCCAGCGTCCTTCAGAGAGGCTAGCTGTACCCAGGGAGGCACTACCAGAGAATCAAGTGTGAGGAGT
  AGCCCAGGCTTCCAGCCTACAACCATGGAAGGCTCACCCACGGGCAGCTCATCCCTGCTGTCATCAGAAC
  CACCACAGATCATCATCAACCCAGCCCGACAGAAGATGGTACAAAAGCTGGCTCTTTATGAAGAAGGGGT
  CTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGGCTTGGATTTAGAACCTGAAAAGAGCCAG
  GGACCTGAAGAAAGTGATGAATTCCAGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227189 representing NM_008363
  Red=Cloning site Green=Tags(s)
  
  MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVL
  WPWINRNARVADLVHILTHLQLLRARDIITAWHPPAPVVPPSTAAPRPSSISAGSEAGDWSPRKLQSSAS
  TFLSPAFPGSQTHSESELLQVPLPVSLGPPLPSSAPSSTKSSPESPVSGLQRAHPSPFCWPFCEISQGTC
  NFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTMVKQSFLTEVEQLSRFRHPNIVDFAGYCAE
  SGLYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDE
  RLMPKLGDFGLARFSRFAGAKASQSSTVARTSTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAG
  QRAVRTQGAKTKYLKDLIEDEAEEAGVTLKSTQPTLWVGVATDAWAAPIAAQIYKKHLDSRPGPCPPQLG
  LALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAGPPWELEVAGHGSPSPQENSYMSTTGSAQSGDEPWQP
  LVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPGSHPSPASFREASCTQGGTTRESSVRS
  SPGFQPTTMEGSPTGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLDLEPEKSQ
  GPEESDEFQS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008363
• ORF Size: 2130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008363.2, NP_032389.2
• RefSeq Size: 3831
• RefSeq ORF: 2133
• Locus ID: 16179