Prf1 (NM_011073) Mouse Tagged ORF Clone

CAT#: MG227213

Prf1 (GFP-tagged) - Mouse perforin 1 (pore forming protein) (Prf1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prf1
• Synonyms: Pfn; Pfp; Prf-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227213 representing NM_011073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGTGCCTGTTCCTCCTGGGCCTTTTCCTGCTGCTGCCACGACCTGTCCCTGCTCCCTGCTACA
  CTGCCACTCGGTCAGAATGCAAGCAGAAGCACAAGTTCGTGCCAGGTGTATGGATGGCTGGGGAAGGCAT
  GGATGTGACTACCCTCCGCCGCTCCGGCTCCTTCCCAGTGAACACACAGAGGTTCCTGAGGCCTGACCGC
  ACCTGCACCCTCTGTAAAAACTCCCTAATGAGAGACGCCACACAGCGCCTACCTGTGGCAATCACCCACT
  GGCGGCCTCACAGCTCACACTGCCAGCGTAATGTGGCCGCAGCCAAGGTCCACTCCACGGAGGGTGTGGC
  CCGGGAGGCAGCTGCTAATATCAATAACGACTGGCGTGTGGGGCTGGATGTGAACCCTAGGCCAGAGGCA
  AACATGCGCGCCTCCGTGGCTGGCTCCCACTCCAAGGTAGCCAATTTTGCAGCTGAGAAGACCTATCAGG
  ACCAGTACAACTTTAATAGCGACACAGTAGAGTGTCGCATGTACAGTTTTCGCCTGGTACAAAAACCTCC
  ACTCCACCTTGACTTCAAAAAGGCGCTCAGAGCCCTCCCCCGCAACTTTAACAGCTCCACAGAGCATGCT
  TACCACAGGCTCATCTCCTCCTATGGCACGCACTTTATCACGGCTGTGGACCTCGGTGGCCGCATCTCGG
  TCCTTACAGCCCTGCGTACCTGTCAGCTGACCCTGAATGGGCTCACAGCTGATGAGGTAGGAGACTGCCT
  GAACGTGGAGGCCCAGGTCAGCATCGGTGCCCAAGCCAGCGTCTCCAGTGAATACAAAGCTTGTGAGGAG
  AAGAAGAAACAGCACAAAATGGCCACCTCTTTCCACCAGACCTACCGTGAGCGTCACGTCGAAGTACTTG
  GTGGCCCTCTGGACTCCACGCATGATCTGCTCTTCGGGAACCAAGCTACACCAGAGCAGTTCTCAACCTG
  GACAGCCTCACTGCCCAGCAACCCTGGTCTGGTGGACTACAGCCTGGAGCCCCTGCACACATTACTGGAA
  GAACAGAACCCGAAGCGGGAGGCTCTGAGACAGGCTATCAGCCATTATATAATGAGCAGAGCCCGGTGGC
  AGAACTGTAGCAGGCCCTGCAGGTCAGGCCAGCATAAGAGTAGCCATGATTCATGCCAGTGTGAGTGCCA
  GGATTCAAAGGTCACCAACCAGGACTGCTGCCCACGACAGAGGGGCTTGGCCCATTTGGTGGTAAGCAAT
  TTCCGGGCAGAACATCTGTGGGGAGACTACACCACAGCTACTGATGCCTACCTAAAGGTCTTCTTTGGTG
  GCCAGGAGTTCAGGACCGGTGTCGTGTGGAACAATAACAATCCCCGGTGGACTGACAAGATGGACTTTGA
  GAATGTGCTCCTGTCCACAGGGGGACCCCTCAGGGTGCAGGTCTGGGATGCCGACTACGGCTGGGATGAT
  GACCTTCTTGGTTCTTGTGACAGGTCTCCCCACTCTGGTTTCCATGAGGTGACATGTGAGCTAAACCACG
  GCAGGGTGAAATTCTCCTACCATGCCAAGTGTCTGCCCCATCTCACTGGAGGGACCTGCCTGGAGTATGC
  CCCCCAGGGGCTTCTGGGAGATCCTCCAGGAAACCGCAGTGGGGCTGTGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227213 representing NM_011073
  Red=Cloning site Green=Tags(s)
  
  MATCLFLLGLFLLLPRPVPAPCYTATRSECKQKHKFVPGVWMAGEGMDVTTLRRSGSFPVNTQRFLRPDR
  TCTLCKNSLMRDATQRLPVAITHWRPHSSHCQRNVAAAKVHSTEGVAREAAANINNDWRVGLDVNPRPEA
  NMRASVAGSHSKVANFAAEKTYQDQYNFNSDTVECRMYSFRLVQKPPLHLDFKKALRALPRNFNSSTEHA
  YHRLISSYGTHFITAVDLGGRISVLTALRTCQLTLNGLTADEVGDCLNVEAQVSIGAQASVSSEYKACEE
  KKKQHKMATSFHQTYRERHVEVLGGPLDSTHDLLFGNQATPEQFSTWTASLPSNPGLVDYSLEPLHTLLE
  EQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCCPRQRGLAHLVVSN
  FRAEHLWGDYTTATDAYLKVFFGGQEFRTGVVWNNNNPRWTDKMDFENVLLSTGGPLRVQVWDADYGWDD
  DLLGSCDRSPHSGFHEVTCELNHGRVKFSYHAKCLPHLTGGTCLEYAPQGLLGDPPGNRSGAVW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011073
• ORF Size: 1662 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011073.3, NP_035203.3
• RefSeq Size: 2054
• RefSeq ORF: 1665
• Locus ID: 18646