Adora3 (NM_009631) Mouse Tagged ORF Clone

CAT#: MG227237

Adora3 (GFP-tagged) - Mouse adenosine A3 receptor (Adora3) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adora3
• Synonyms: A3AR; A3R; AA3R; ARA3; Gpcr2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227237 representing NM_009631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCCGACAACACCACGGAGACGGACTGGCTGAACATCACCTACATCACCATGGAGGCTGCCATCG
  GGCTCTGTGCCGTTGTGGGCAACATGCTGGTCATCTGGGTGGTCAAGCTGAACCCCACTCTGAGGACCAC
  CACGTTCTATTTCATTGTCTCCCTAGCACTGGCTGACATTGCCGTTGGGGTGCTGGTCACACCTTTGGCC
  ATTGCTGTCAGCCTGCAAGTCAAGATGCACTTCTATGCCTGCCTTTTCATGTCCTGTGTGCTGCTGATCT
  TCACCCATGCTTCCATCATGTCCTTGCTGGCCATTGCTGTAGACCGATACCTGCGGGTCAAGCTGACAGT
  CAGATATAGAACGGTTACCACTCAAAGAAGAATATGGCTATTCTTGGGCCTTTGCTGGCTAGTTTCCTTT
  CTGGTGGGGCTGACCCCCATGTTTGGCTGGAATAGAAAAGCAACCTTAGCGAGCTCTCAAAATAGCAGCA
  CTCTTTTGTGCCACTTCCGTTCCGTGGTCAGTTTGGATTACATGGTCTTCTTCAGCTTCGTCACCTGGAT
  CCTCGTCCCCCTGGTTGTCATGTGTGTCATCTACCTAGACATCTTCTACATCATCCGAAATAAGCTCAGT
  CAAAACCTGTCTGGCTTCAGAGAGACGCGTGCATTTTATGGACGGGAGTTCAAGACAGCTAAGTCCCTGT
  TTCTGGTTCTCTTCTTGTTTGCGCTGTGCTGGCTGCCTTTGTCCATCATCAATTTTGTTTCCTATTTTGA
  TGTAAAGATACCAGATGTCGCAATGTGCCTGGGGATCCTGTTGTCCCACGCGAACTCCATGATGAACCCT
  ATTGTCTACGCCTGCAAAATAAAAAAGTTCAAAGAGACCTACTTTCTGATCCTCAGAGCTCTCAGGCTCT
  GTCAGACCTCAGATTCTTTGGACTCAAACATGGAACAGACTACTGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227237 representing NM_009631
  Red=Cloning site Green=Tags(s)
  
  MEADNTTETDWLNITYITMEAAIGLCAVVGNMLVIWVVKLNPTLRTTTFYFIVSLALADIAVGVLVTPLA
  IAVSLQVKMHFYACLFMSCVLLIFTHASIMSLLAIAVDRYLRVKLTVRYRTVTTQRRIWLFLGLCWLVSF
  LVGLTPMFGWNRKATLASSQNSSTLLCHFRSVVSLDYMVFFSFVTWILVPLVVMCVIYLDIFYIIRNKLS
  QNLSGFRETRAFYGREFKTAKSLFLVLFLFALCWLPLSIINFVSYFDVKIPDVAMCLGILLSHANSMMNP
  IVYACKIKKFKETYFLILRALRLCQTSDSLDSNMEQTTE
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009631
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009631.3, NP_033761.2
• RefSeq Size: 1797
• RefSeq ORF: 960
• Locus ID: 11542
• Gene Summary: This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. This gene shares its 3' terminal exon with a transcript variant from overlapping GeneID:69296, which encodes an immunoglobulin domain-containing protein. [provided by RefSeq, Nov 2014]