Hsd11b1 (NM_008288) Mouse Tagged ORF Clone

CAT#: MG227258

Hsd11b1 (GFP-tagged) - Mouse hydroxysteroid 11-beta dehydrogenase 1 (Hsd11b1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hsd11b1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227258 representing NM_008288
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTTATGAAAAATTACCTCCTCCCGATCCTGGTGCTCTTCCTGGCCTACTACTACTATTCTACAA
  ATGAAGAGTTCAGACCAGAAATGCTCCAGGGAAAGAAAGTGATTGTCACTGGGGCCAGCAAAGGGATTGG
  AAGAGAAATGGCATATCATCTGTCAAAAATGGGAGCCCATGTGGTATTGACTGCCAGGTCGGAGGAAGGT
  CTCCAGAAGGTAGTGTCTCGCTGCCTTGAACTCGGAGCAGCCTCTGCTCACTACATTGCTGGCACTATGG
  AAGACATGACATTTGCGGAGCAATTTATTGTCAAGGCGGGAAAGCTCATGGGCGGACTGGACATGCTTAT
  TCTAAACCACATCACTCAGACCTCGCTGTCTCTCTTCCATGACGACATCCACTCTGTGCGAAGAGTCATG
  GAGGTCAACTTCCTCAGCTACGTGGTCATGAGCACAGCCGCCTTGCCCATGCTGAAGCAGAGCAATGGCA
  GCATTGCCGTCATCTCCTCCTTGGCTGGGAAAATGACCCAGCCTATGATTGCTCCCTACTCTGCAAGCAA
  GTTTGCTCTGGATGGGTTCTTTTCCACCATTAGAACAGAACTCTACATAACCAAGGTCAACGTGTCCATC
  ACTCTCTGTGTCCTTGGCCTCATAGACACAGAAACAGCTATGAAGGAAATCTCTGGGATAATTAACGCCC
  AAGCTTCTCCCAAGGAGGAGTGCGCCCTGGAGATCATCAAAGGCACAGCTCTACGCAAAAGCGAGGTGTA
  CTATGACAAATCGCCTTTGACTCCAATCCTGCTTGGGAACCCAGGAAGGAAGATCATGGAATTTTTTTCA
  TTACGATATTATAATAAGGACATGTTTGTAAGTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227258 representing NM_008288
  Red=Cloning site Green=Tags(s)
  
  MAVMKNYLLPILVLFLAYYYYSTNEEFRPEMLQGKKVIVTGASKGIGREMAYHLSKMGAHVVLTARSEEG
  LQKVVSRCLELGAASAHYIAGTMEDMTFAEQFIVKAGKLMGGLDMLILNHITQTSLSLFHDDIHSVRRVM
  EVNFLSYVVMSTAALPMLKQSNGSIAVISSLAGKMTQPMIAPYSASKFALDGFFSTIRTELYITKVNVSI
  TLCVLGLIDTETAMKEISGIINAQASPKEECALEIIKGTALRKSEVYYDKSPLTPILLGNPGRKIMEFFS
  LRYYNKDMFVSN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008288
• ORF Size: 876 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008288.2, NP_032314.2
• RefSeq Size: 1381
• RefSeq ORF: 879
• Locus ID: 15483
• Gene Summary: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. [UniProtKB/Swiss-Prot Function]