F5 (NM_007976) Mouse Tagged ORF Clone
CAT#: MG227261
- TrueORF®
F5 (GFP-tagged) - Mouse coagulation factor V (F5), (10ug)
"NM_007976" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | F5 |
Synonyms | AI173222; Cf-5; Cf5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG227261 representing NM_007976
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTAGTCTGCCCGTGCTTCTTCCTCCTGGTGGTTCTGGGAACCCGCTGGGCGGGCTGGGGCAGCC ACCAGGCAGAGGCCGCGCAACTAAGGCAGTTCTATGTGGCAGCTCAGGGGATCCTCTGGAACTATCATCC TGAGCCCACAGATCCAAGTTTGAATTCTATACCTTCCTTCAAGAAAATTGTCTACAGAGAGTATGAACAG TATTTTAAGAAAGAAAAGCCACGATCTAGCAACTCAGGACTTCTTGGACCTACTTTATACGCTGAAGTTG GGGACGTCATTAAAGTTCACTTTAGAAACAAAGCAGACAAACCACTAAGCATCCATCCTCAAGGGATTAA ATACAGTAAATTTTCAGAAGGGGCTTCTTACGCAGACCACACGTTCCCTGCCGAGAGGAAGGATGATGCC GTGGCTCCTGGAGAAGAATACACCTATGAATGGATCGTCAGTGAGGACAGCGGGCCCACACCTGATGACC CACCATGCCTCACCCACATCTACTATTCCTATGAAAACCTGACCCAGGATTTCAACTCGGGTCTGATTGG GCCTCTGCTTATCTGCAAGAAAGGCACCCTGACCGAGGATGGGACTCAGAAGATGTTTGACAAGCAGCAT GTGCTCCTATTTGCTGTGTTTGATGAAAGCAAGAGCCGGAGCCAGTCACCATCCCTAATGTACACAATTA ATGGCTTTGTGAATAAGACGATGCCAGATATAACAGTCTGTGCCCATGACCACGTCAGCTGGCATCTGAT CGGGATGAGCTCGGGGCCAGAATTGTTTTCTATTCACTTCAACGGCCAAGTCCTAGAGCAGAACCAGCAT AAAGTGTCCACCGTCACCCTGGTCAGCGCAACATCTACGACTGCAAACATGACTATGAGCCCAGAAGGAA GATGGATTGTTTCTTCTCTCATCCCAAAGCATTATCAAGCTGGGATGCAGGCTTACATTGACATTAAAAA CTGCCCAAAGAAAACGAGGAGCCCCAAGACCCTCACTCGGGAGCAGAGGCGGTACATGAAGAGATGGGAG TATTTCATAGCCGCAGAGGAGGTCATTTGGAACTATGCACCCGTGATACCTGCGAATATGGACAAAATTT ACAGGTCTCAGCACTTGGATAATTTCTCAAACCAAATTGGAAAACATTACAAGAAAGTTATCTACAGGCA ATATGAAGAAGAGACCTTCACCAAACGCACTGACAACCCCAGCATCAAACAAAGTGGGATTCTGGGCCCT GTTATCAGAGCCCAGGTCAGAGACACACTCAAGATCGTGTTCAAAAATATGGCGAGCCGACCCTACAGCA TTTACCCTCACGGGGTGACCTTCTCTCCTTACGAAGATGGAATCAATTCTTCCTCCACCTCAGGCAGTCA CACCACGATCAGACCAGTTCAACCGGGGGAAACCTTCACTTACAAATGGAACATTCTAGAGTTTGATGAA CCCACGGAAAACGATGCCCAGTGCCTAACAAGGCCATACTACAGTGATGTGGACGTTACAAGGGATATTG CCTCTGGGCTGATAGGGCTGCTTCTAATTTGTAAGAGCAGGTCCCTGGACCAGAGGGGTGTACAGAGGGT GGCAGACATCGAGCAGCAGGCCGTGTTTGCTGTGTTTGACGAGAACAAGAGCTGGTACATTGAGGACAAC ATCAACAAGTTCTGTGAGAATCCTGATGAGGTGAAGCGTGATGATCCCAAGTTTTACGAATCAAACATCA TGAGCACTATCAACGGCTACGTGCCCGAGAGCATTTCCACTCTGGGATTCTGTTTTGATGACACTGTCCA GTGGCACTTCTGCAGTGTGGGAACTCATGATGATATTTTGACCATCCACTTCACTGGGCACTCGTTCATC TATGGGAGGAGGCACGAGGACACCTTGACCCTGTTCCCCATGCGTGGTGAATCTGTGACAGTTACAATGG ATAATGTTGGAACTTGGATGTTGACCACCATGAATTCCAATCCAAAACGCAGAAACCTAAGACTGAGATT CAGAGATGTTAAGTGTAATCGGGATTATGACAATGAGGACTCATATGAGATTTATGAACCTCCTGCACCT ACATCCATGACAACTCGGAGAATTCATGATTCCTTAGAAAATGAATTTGGCATAGACAACGAAGATGATG ATTACCAGTACTTACTGGCGTCATCATTAGGAATTAGGTCATTCAAAAACTCATCATTGAATCCAGAGGA AAATGAGTTCAATCTCACTGCTCTCGCTCTGGAGAACAGCTCTGAGTTCATATCTCCAAGCACAGACAGA GTTGTTGACTCAAACTCTTCACGAATCCTTAGTAAAATCATCAATAATAACCTCAAAGACTTTCAAAGAA CACTTCCTGGCTCAGGAGCCACCGTGGCTGGTACCCTCCTTAGAAACCTCATTGGCTTAGATGAGAACTT CGTCCTCAACTCTTCTACAGAACATCGTTCCAGCTCATATCATGAAAATGATATGGAAAATCCACAGTCA AACATCACAATGGTATACCTACTTCCTCTTGGTCCAAAAGGATCTGGGAATCGAGAACAAGATAAACCTA AAACCATCAAGACAGGAAGACCCCACATGATGAAGCACAGGTTCTCCTGGATGAAAGCGCCAGCTGGTAA AACTGGGAGGCATTCAAACCCAAAGAATTCGTATTCTGGAATGAAGTCTGAGGAGGACATTCCTAGCGAG TTGATACCCTTAAAGCAAAAGATCACTTCCAAATTTCTGAATAGACGATGGCGTGTGGCTTCTGAAAAGG GTAGTTATGAAATAATAGCAGCAAATGGTGAAGACACAGATGTGGATAAGCTGACCAACAGTCCTCAAAA TCAGAATATCACAGTACCTCGGGGAGAGAGCACCTCTCACACAAACACAACAAGAAAGCCAAGTGACCTC CCAACATTTTCTGGAGTTGGACATAAATCTCCACATGTAAGACAGGAGGAAGAAAACAGTGGTTTTCAGA AAAGACAGTTATTCATCAGGACACGGAAGAAGAAGAAAAATAAGAAGCTTGCACTACACAGTCCTCTATC TCCAAGGGGCTTTGACCCTTTGAGAGGACATAACCATTCCCCATTTCCAGACAGGAGACTACTTAATCAC TCACTGTTACTCCACAAGTCCAATGAAACAGCTCTTTCTCCAGACCTGAACCAGACCTCTCCTTCAATGA GTACGGACAGGTCACTTCCTGACTATAATCAGTACTCGAAAAATGACACTGAGCAGATGAGCTCTTCTTT AGATCTTTATCAGTCAGTGCCCGCAGAGGAACACTCTCCAACATTTCCTGCCCAAGATCCTGATCAAACA CACTCTACCACAGATCCTAGCTACAGATCCTCTCCGCCAGAGCTCAGCCAGGGGCTTGATTATGACCTAA GTCATGACTTTTACCCTGATGACATTGGTCTAACATCTTTCTTTCCAGACCAAAGTCAAAAGTCATCTTT CTCTTCAGATGATGACCAAGCAATCCCTTCCTCAGACTTAAGCCTCTTTACCATCTCTCCAGAATTGGAT CAGACAATTATTTACCCAGACCTGGATCAGTTGCTCCTTTCTCCAGAAGACAATCAGAAGACCTCCTCCC CAGACCTGGGCCAGGTGCCCCTTTCTCCAGATGACAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGT GTCCCTTTCTCCAGATGATAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCTAGAT GACAACCAGAAGACGACCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCCAGATGACAACCAGATGATCA CCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTTCTGATAACCAGAAGACCTCTTCCCCAGATCTGGGTCA GGTGCCTCTTTTTCCTGAAGACAACCAGAATTACTTCCTAGACCTGAGTCAGGTACCTCTCTCCTCAGAC CAAAACCAGGAGACCTCCTCCACAGACCTACTGACTCTCTCTCCTGATTTTGGTCAGACAGTCCTTTCCC CAGACTTGGATCAGCTGCCACTCCCTTCAGACAATAGTCAGGTGACCGTTTCCCCAGACCTCAGCCTCTT GACCCTCTCACCAGATTTTAATGAGATAATCCTAGCCCCAGACCTTGGTCAAGTGACCCTCTCTCCAGAC CTCATCCAGACAAACCCTGCTCTTAATCATGGACACAAAGCATCCTCTGCAGACCCTGATCAAGCATCCT ACCCTCCAGATTCTGGTCAGGCTTCATCGCTTCCAGAACTGAATCGGACTCTTCCTCATCCAGATCTCAC TCACATACCACCTCCTTCACCATCTCCCACACTCAATAACACTTCTTTGTCAAGGAAATTTAACCCTCTT GTTGTAGTAGGTCTCAGTAGAGTAGATGGAGACGACGTTGAGATTGTTCCAAGTGAGGAGCCAGAGAGAA TAGATGAAGATTATGCCGAGGATGACTTTGTAACCTATAATGACCCCTACAGAACAGACACTAGGACAGA TGTCAATTCCTCCAGAAATCCTGACACTATCGCAGCATGGTACCTCCGAGGCCACGGTGGACACAAAAAA TTCTACTATATTGCAGCTGAAGAAATAACCTGGAATTACGCAGAGTTTGCACAAAGTGAAATGGACCATG AAGACACAGGCCACACTCCAAAGGACACCACATACAAGAAAGTCGTTTTCAGAAAATACCTTGATAGCAC GTTTACAAGTCGTGATCCTCGGGCAGAATATGAGGAGCACCTTGGCATTCTCGGTCCTGTGATCCGGGCT GAAGTGGATGATGTGATCCAAGTTCGATTTAAAAATTTGGCATCCAGACCGTATTCTCTTCATGCTCACG GACTTTCCTATGAAAAATCCTCAGAGGGGAAGACTTATGAAGATGAATCTCCTGAATGGTTTCAGGAAGA TGATGCTGTCCAGCCCAATAGCAGTTACACCTATGTATGGCATGCCACCAAGCGCTCAGGGCCAGAGAAC CCTGGTTCTGCCTGCCGGGCTTGGGCCTACTATTCTGCAGTGAATGTGGAGAGGGACATCCACTCAGGCT TGATCGGCCCCCTTCTGATCTGCCGGAAAGGAACACTTCACATGGAGCGCAACCTGCCTATGGACATGAG AGAGTTTGTCTTACTCTTCATGGTCTTTGATGAGAAGAAGAGCTGGTACTATGAAAAGTCCAAGGGGTCA CGGAGAATTGAATCCCCAGAAGAGAAAAATGCCCACAAGTTTTACGCAATTAATGGGATGATCTACAACC TGCCCGGCCTGAGAATGTACGAGCAAGAGTGGGTGAGGCTACACCTGCTGAACATGGGCGGCTCCCGAGA TATTCACGTGGTTCACTTCCATGGCCAGACCCTGCTGGATAATAGGACCAAACAGCACCAGTTAGGCGTC TGGCCCCTTCTGCCTGGTTCATTTAAAACTCTTGAAATGAAGGCATCCAAGCCTGGCTGGTGGCTCCTAG ACACAGAGGTTGGAGAAAACCAGGTAGCTGGCATGCAAACGCCATTTCTCATCATAGACAAAGAGTGTAA GATGCCAATGGGACTAAGCACTGGTGTCATATCTGATTCACAGATCAAGGCTTCGGAATATCTGACTTAT TGGGAGCCCAGATTAGCACGATTAAACAATGCTGGTTCATACAATGCTTGGAGTATAGAAAAAACTGCAT TAGATTTTCCCATTAAACCTTGGATCCAGGTGGACATGCAGAAGGAAGTTGTAGTCACCGGGATACAAAC CCAAGGTGCTAAACACTACCTAAAGTCCTGCTTTACCACGGAGTTCCAAGTGGCTTACAGCTCTGACCAA ACCAACTGGCAGATCTTCAGAGGGAAGAGCGGGAAGAGCGTGATGTATTTTACTGGTAATTCAGATGGCT CTACAATAAAAGAGAATCGACTTGACCCACCCATTGTGGCTAGATACATTAGGATACACCCAACAAAATC CTATAATAGACCCACCCTTCGGCTGGAGCTGCAGGGCTGTGAGGTGAACGGATGTTCCACACCACTGGGC CTGGAAGATGGACGGATTCAAGACAAGCAAATTACTGCATCTTCATTTAAAAAGTCGTGGTGGGGAGACT ACTGGGAGCCCTCCCTTGCCCGCCTGAACGCCCAGGGCCGCGTGAACGCCTGGCAAGCCAAGGCAAACAA CAACAAGCAGTGGTTACAAGTCGATCTGCTCAAAATCAAGAAGGTAACGGCCATCGTAACGCAGGGCTGT AAGTCTCTGTCCTCTGAGATGTACGTGAAGAGCTACAGCATCCAGTACAGTGACCAGGGTGTGGCATGGA AACCTTACCGACAGAAATCCTCCATGGTGGACAAGATTTTTGAAGGAAACAGCAATACCAAGGGGCACAT GAAGAACTTTTTCAACCCGCCCATTATTTCCAGATTTATCCGCATCATTCCTAAAACATGGAACCAGAGC ATCGCCCTTCGCCTAGAGCTCTTCGGCTGTGACATTTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227261 representing NM_007976
Red=Cloning site Green=Tags(s) MLLVCPCFFLLVVLGTRWAGWGSHQAEAAQLRQFYVAAQGILWNYHPEPTDPSLNSIPSFKKIVYREYEQ YFKKEKPRSSNSGLLGPTLYAEVGDVIKVHFRNKADKPLSIHPQGIKYSKFSEGASYADHTFPAERKDDA VAPGEEYTYEWIVSEDSGPTPDDPPCLTHIYYSYENLTQDFNSGLIGPLLICKKGTLTEDGTQKMFDKQH VLLFAVFDESKSRSQSPSLMYTINGFVNKTMPDITVCAHDHVSWHLIGMSSGPELFSIHFNGQVLEQNQH KVSTVTLVSATSTTANMTMSPEGRWIVSSLIPKHYQAGMQAYIDIKNCPKKTRSPKTLTREQRRYMKRWE YFIAAEEVIWNYAPVIPANMDKIYRSQHLDNFSNQIGKHYKKVIYRQYEEETFTKRTDNPSIKQSGILGP VIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDGINSSSTSGSHTTIRPVQPGETFTYKWNILEFDE PTENDAQCLTRPYYSDVDVTRDIASGLIGLLLICKSRSLDQRGVQRVADIEQQAVFAVFDENKSWYIEDN INKFCENPDEVKRDDPKFYESNIMSTINGYVPESISTLGFCFDDTVQWHFCSVGTHDDILTIHFTGHSFI YGRRHEDTLTLFPMRGESVTVTMDNVGTWMLTTMNSNPKRRNLRLRFRDVKCNRDYDNEDSYEIYEPPAP TSMTTRRIHDSLENEFGIDNEDDDYQYLLASSLGIRSFKNSSLNPEENEFNLTALALENSSEFISPSTDR VVDSNSSRILSKIINNNLKDFQRTLPGSGATVAGTLLRNLIGLDENFVLNSSTEHRSSSYHENDMENPQS NITMVYLLPLGPKGSGNREQDKPKTIKTGRPHMMKHRFSWMKAPAGKTGRHSNPKNSYSGMKSEEDIPSE LIPLKQKITSKFLNRRWRVASEKGSYEIIAANGEDTDVDKLTNSPQNQNITVPRGESTSHTNTTRKPSDL PTFSGVGHKSPHVRQEEENSGFQKRQLFIRTRKKKKNKKLALHSPLSPRGFDPLRGHNHSPFPDRRLLNH SLLLHKSNETALSPDLNQTSPSMSTDRSLPDYNQYSKNDTEQMSSSLDLYQSVPAEEHSPTFPAQDPDQT HSTTDPSYRSSPPELSQGLDYDLSHDFYPDDIGLTSFFPDQSQKSSFSSDDDQAIPSSDLSLFTISPELD QTIIYPDLDQLLLSPEDNQKTSSPDLGQVPLSPDDNQKTSSPDLGQVSLSPDDNQKTSSPDLGQVPLSLD DNQKTTSPDLGQVPLSPDDNQMITSPDLGQVPLSSDNQKTSSPDLGQVPLFPEDNQNYFLDLSQVPLSSD QNQETSSTDLLTLSPDFGQTVLSPDLDQLPLPSDNSQVTVSPDLSLLTLSPDFNEIILAPDLGQVTLSPD LIQTNPALNHGHKASSADPDQASYPPDSGQASSLPELNRTLPHPDLTHIPPPSPSPTLNNTSLSRKFNPL VVVGLSRVDGDDVEIVPSEEPERIDEDYAEDDFVTYNDPYRTDTRTDVNSSRNPDTIAAWYLRGHGGHKK FYYIAAEEITWNYAEFAQSEMDHEDTGHTPKDTTYKKVVFRKYLDSTFTSRDPRAEYEEHLGILGPVIRA EVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDESPEWFQEDDAVQPNSSYTYVWHATKRSGPEN PGSACRAWAYYSAVNVERDIHSGLIGPLLICRKGTLHMERNLPMDMREFVLLFMVFDEKKSWYYEKSKGS RRIESPEEKNAHKFYAINGMIYNLPGLRMYEQEWVRLHLLNMGGSRDIHVVHFHGQTLLDNRTKQHQLGV WPLLPGSFKTLEMKASKPGWWLLDTEVGENQVAGMQTPFLIIDKECKMPMGLSTGVISDSQIKASEYLTY WEPRLARLNNAGSYNAWSIEKTALDFPIKPWIQVDMQKEVVVTGIQTQGAKHYLKSCFTTEFQVAYSSDQ TNWQIFRGKSGKSVMYFTGNSDGSTIKENRLDPPIVARYIRIHPTKSYNRPTLRLELQGCEVNGCSTPLG LEDGRIQDKQITASSFKKSWWGDYWEPSLARLNAQGRVNAWQAKANNNKQWLQVDLLKIKKVTAIVTQGC KSLSSEMYVKSYSIQYSDQGVAWKPYRQKSSMVDKIFEGNSNTKGHMKNFFNPPIISRFIRIIPKTWNQS IALRLELFGCDIY TRTRRLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007976 |
ORF Size | 6549 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_007976.2 |
RefSeq Size | 7406 |
RefSeq ORF | 6552 |
Locus ID | 14067 |
Gene Summary | This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015] |
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