Sirt1 (NM_019812) Mouse Tagged ORF Clone

CAT#: MG227263

Sirt1 (GFP-tagged) - Mouse sirtuin 1 (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) (Sirt1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sirt1
• Synonyms: AA673258; Sir2; Sir2a; Sir2alpha; SIR2L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227263 representing NM_019812
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGAGGTGGCGCTCGCCCTTCAGGCCGCCGGCTCCCCTTCCGCGGCGGCCGCCATGGAGGCCG
  CGTCGCAGCCGGCGGACGAGCCGCTCCGCAAGAGGCCCCGCCGAGACGGGCCTGGCCTCGGGCGCAGCCC
  GGGCGAGCCGAGCGCAGCAGTGGCGCCGGCGGCCGCGGGGTGTGAGGCGGCGAGCGCCGCGGCCCCGGCG
  GCGCTGTGGCGGGAGGCGGCAGGGGCGGCGGCGAGCGCGGAGCGGGAGGCCCCGGCGACGGCCGTGGCCG
  GGGACGGAGACAATGGGTCCGGCCTGCGGCGGGAGCCGAGGGCGGCTGACGACTTCGACGACGACGAGGG
  CGAGGAGGAGGACGAGGCGGCGGCGGCAGCGGCGGCGGCAGCGATCGGCTACCGAGACAACCTCCTGTTG
  ACCGATGGACTCCTCACTAATGGCTTTCATTCCTGTGAAAGTGATGACGATGACAGAACGTCACACGCCA
  GCTCTAGTGACTGGACTCCGCGGCCGCGGATAGGTCCATATACTTTTGTTCAGCAACATCTCATGATTGG
  CACCGATCCTCGAACAATTCTTAAAGATTTATTACCAGAAACAATTCCTCCACCTGAGCTGGATGATATG
  ACGCTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAGCGGAAAAAAAGAAAAGATATCAATA
  CAATTGAAGATGCTGTGAAGTTACTGCAGGAGTGTAAAAAGATAATAGTTCTGACTGGAGCTGGGGTTTC
  TGTCTCCTGTGGGATTCCTGACTTCAGATCAAGAGACGGTATCTATGCTCGCCTTGCGGTGGACTTCCCA
  GACCTCCCAGACCCTCAAGCCATGTTTGATATTGAGTATTTTAGAAAAGACCCAAGACCATTCTTCAAGT
  TTGCAAAGGAAATATATCCCGGACAGTTCCAGCCGTCTCTGTGTCACAAATTCATAGCTTTGTCAGATAA
  GGAAGGAAAACTACTTCGAAATTATACTCAAAATATAGATACCTTGGAGCAGGTTGCAGGAATCCAAAGG
  ATCCTTCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTTGTAAATACAAAGTTGATTGTGAAG
  CTGTTCGTGGAGACATTTTTAATCAGGTAGTTCCTCGGTGCCCTAGGTGCCCAGCTGATGAGCCACTTGC
  CATCATGAAGCCAGAGATTGTCTTCTTTGGTGAAAACTTACCAGAACAGTTTCATAGAGCCATGAAGTAT
  GACAAAGATGAAGTTGACCTCCTCATTGTTATTGGATCTTCTCTGAAAGTGAGACCAGTAGCACTAATTC
  CAAGTTCTATACCCCATGAAGTGCCTCAAATATTAATAAATAGGGAACCTTTGCCTCATCTACATTTTGA
  TGTAGAGCTCCTTGGAGACTGCGATGTTATAATTAATGAGTTGTGTCATAGGCTAGGTGGTGAATATGCC
  AAACTTTGTTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAAACCTCCACGCCCACAAAAGGAATTGG
  TTCATTTATCAGAGTTGCCACCAACACCTCTTCATATTTCGGAAGACTCAAGTTCACCTGAAAGAACTGT
  ACCACAAGACTCTTCTGTGATTGCTACACTTGTAGACCAAGCAACAAACAACAATGTTAATGATTTAGAA
  GTATCTGAATCAAGTTGTGTGGAAGAAAAACCACAAGAAGTACAGACTAGTAGGAATGTTGAGAACATTA
  ATGTGGAAAATCCAGATTTTAAGGCTGTTGGTTCCAGTACTGCAGACAAAAATGAAAGAACTTCAGTTGC
  AGAAACAGTGAGAAAATGCTGGCCTAATAGACTTGCAAAGGAGCAGATTAGTAAGCGGCTTGAGGGTAAT
  CAATACCTGTTTGTACCACCAAATCGTTACATATTCCACGGTGCTGAGGTATACTCAGACTCTGAAGATG
  ACGTCTTGTCCTCTAGTTCCTGTGGCAGTAACAGTGACAGTGGCACATGCCAGAGTCCAAGTTTAGAAGA
  ACCCTTGGAAGATGAAAGTGAAATTGAAGAATTCTACAATGGCTTGGAAGATGATACGGAGAGGCCCGAA
  TGTGCTGGAGGATCTGGATTTGGAGCTGATGGAGGGGATCAAGAGGTTGTTAATGAAGCTATAGCTACAA
  GACAGGAATTGACAGATGTAAACTATCCATCAGACAAATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227263 representing NM_019812
  Red=Cloning site Green=Tags(s)
  
  MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGRSPGEPSAAVAPAAAGCEAASAAAPA
  ALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLL
  TDGLLTNGFHSCESDDDDRTSHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDM
  TLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFP
  DLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQR
  ILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKY
  DKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYA
  KLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLE
  VSESSCVEEKPQEVQTSRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGN
  QYLFVPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLEDDTERPE
  CAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019812
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019812.2, NP_062786.1
• RefSeq Size: 3920
• RefSeq ORF: 2214
• Locus ID: 93759
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]