Sphk1 (NM_011451) Mouse Tagged ORF Clone

CAT#: MG227335

Sphk1 (GFP-tagged) - Mouse sphingosine kinase 1 (Sphk1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sphk1
• Synonyms: 1110006G24Rik; Sk1; Spk1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227335 representing NM_011451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTGGTGTTGTGTTTTGTTTGTAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGC
  TGGTGCTGCTGAACCCCCAGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTT
  CCTGGAGGAGGCAGAGATAACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTG
  TGTGCAGAGGAGTTGGGTCACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGG
  TGAATGGGCTAATGGAACGGCCAGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGG
  CTCCGGCAATGCGCTGGCAGCTTCTGTGAACCACTATGCTGGGTACGAGCAGGTGACTAATGAAGACCTG
  CTCATCAACTGCACACTGCTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTG
  CTTCTGGGCTGCGGCTCTATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAG
  TGAGAAGTACAGGCGCTTGGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGC
  ATCTACCAAGGCCAACTGGCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGG
  TGCAGAAGGGCCCCGTCGACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGT
  ACCAGAACAGGACTTCGTCCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCA
  CCCATGGGCCGCTGTGAGGCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGC
  TGCTGCGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGT
  GCCCGTGGTTGCTTTCCGCCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATG
  GTATGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCC
  CATCCGGCCGGGACTCCCGGCGGGGGCCACCTCCAGAAGAACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227335 representing NM_011451
  Red=Cloning site Green=Tags(s)
  
  MWWCCVLFVVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELV
  CAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQVTNEDL
  LINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLR
  IYQGQLAYLPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAA
  PMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQRGVFSVDGELM
  VCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011451
• ORF Size: 1164 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011451.3, NP_035581.1
• RefSeq Size: 1537
• RefSeq ORF: 1167
• Locus ID: 20698
• Gene Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]