Psmd1 (NM_027357) Mouse Tagged ORF Clone

CAT#: MG227375

Psmd1 (GFP-tagged) - Mouse proteasome (prosome macropain) 26S subunit non-ATPase 1 (Psmd1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Psmd1
• Synonyms: 2410026J11Rik; P112; S1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227375 representing NM_027357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACTTCAGCCGCTGGAATTATTTCTCTTCTGGATGAAGAAGAGCCACAGCTTAAGGAATTTGCAC
  TACACAAATTGAATGCAGTCGTGAATGACTTCTGGGCAGAGATTTCTGAGTCTGTAGATAAAATAGAAGT
  TTTATATGAAGATGAAGGTTTCCGAAGTCGGCAGTTTGCAGCCCTGGTGGCCTCGAAAGTGTTTTATCAC
  CTGGGGGCTTTTGAGGAGTCTCTGAATTATGCTCTTGGAGCAGGTGACCTCTTCAACGTCAATGATAACT
  CTGAATATGTGGAGACTATTATAGCAAAATGCATTGATCATTATACCAAACAGTGTGTGGAAAATGCAGA
  TTTGCCTGAAGGAGAAAAAAAGCCAATTGACCAGCGATTGGAAGGCATCGTGAATAAAATGTTCCAGCGA
  TGTCTCGATGATCACAAGTATAAGCAAGCGATTGGCATTGCTCTGGAGACCAGGAGACTGGATGTCTTTG
  AGAAGACTATACTGGAATCTAATGATGTCCCAGGAATGCTAGCTTACAGCCTCAAACTCTGCATGTCTTT
  AATGCAGAATAAACAGTTTCGGAATAAAGTATTGAGAGTCTTGGTTAAAATCTACATGAACTTGGAAAAG
  CCTGATTTCATCAATGTTTGTCAGTGCTTAATTTTCTTAGATGATCCTCAGGCTGTGAGTGATATTTTAG
  AAAAACTGGTAAAGGAGGACAACCTGCTGATGGCCTATCAGATTTGTTTTGATTTGTACGAAAGTGCTAG
  CCAGCAGTTCTTGTCATCTGTCATCCAGAATCTTCGAACTGTTGGTACCCCTATTGCTTCTGTGCCTGGA
  TCTACCAATACGGGGACTGTGCCAGGATCAGAGAAAGACAGTGACCCCATGGAGACGGAGGAGAAGACAG
  CCAGTGCAGTGGCTGGGAAGACACCAGATGCGAGTCCAGAGCCCAAGGACCAGACACTGAAAATGATTAA
  GATTTTAAGTGGTGAAATGGCTATTGAATTACACCTGCAGTTCCTAATACGAAATAATAACACAGACCTC
  ATGATTCTGAAAAACACAAAGGATGCAGTACGAAATTCTGTGTGTCATACAGCAACTGTAATAGCAAACT
  CATTCATGCACTGTGGTACTACCAGTGACCAGTTTCTTAGAGATAACTTGGAATGGTTGGCCAGAGCCAC
  CAACTGGGCCAAATTTACTGCAACGGCCAGCTTGGGTGTAATCCATAAGGGCCATGAGAAGGAAGCACTA
  CAGTTAATGGCAACATACCTTCCTAAGGACACTTCTCCAGGATCAGCCTATCAGGAAGGGGGCGGTCTCT
  ATGCATTGGGTCTCATTCATGCCAATCATGGTGGTGACATAATTGACTATCTCCTTAATCAGCTCAAGAA
  TGCCAGCAATGATATTGTTCGACATGGAGGCAGCCTGGGCCTTGGTTTGGCTGCAATGGGAACTGCACGT
  CAGGATGTGTATGATTTGCTGAAAACAAACCTGTATCAGGATGATGCTGTGACAGGGGAGGCAGCTGGCC
  TAGCCTTAGGTTTGGTTATGTTAGGTTCTAAAAATGCCCAGGCGATTGAGGATATGGTTGGCTATGCACA
  AGAGACTCAACACGAGAAGATTCTGCGCGGTCTGGCAGTTGGCATTGCGTTAGTGATGTACGGGAGGATG
  GAGGAGGCTGATGCTCTCATTGAGTCTCTCTGTCGTGACAAGGACCCAATTCTTAGAAGATCTGGAATGT
  ATACTGTAGCCATGGCTTATTGTGGCTCTGGTAACAACAAAGCCATTCGGCGCCTGCTACATGTTGCTGT
  AAGTGATGTCAATGATGATGTCAGAAGAGCAGCAGTGGAGTCACTTGGCTTCATTTTGTTCAGGACGCCT
  GAACAGTGTCCAAGTGTGGTTTCTTTGTTGTCAGAGAGTTATAACCCTCACGTGCGCTATGGAGCTGCAA
  TGGCCCTGGGAATCTGCTGTGCTGGTACAGGAAACAAGGAAGCAATTAATTTACTAGAGCCAATGACCAA
  CGACCCTGTGAACTACGTGAGGCAAGGGGCTCTGATAGCTTCAGCTCTCATCATGATCCAACAGACTGAG
  ATCACGTGTCCAAAGGTGAATCAGTTCCGGCAGCTGTATTCCAAAGTCATCAATGACAAACATGATGATG
  TCATGGCCAAATTTGGCGCTATTCTGGCCCAAGGCATACTGGATGCAGGTGGTCATAATGTCACAATCTC
  CTTACAGTCCAGGACTGGGCACACTCATATGCCGTCTGTGGTTGGCGTCCTTGTCTTCACCCAGTTCTGG
  TTCTGGTTTCCCCTTTCACACTTCCTGTCATTGGCTTATACTCCTACCTGTGTCATTGGCCTTAACAAGG
  ATTTAAAGATGCCGAAAGTTCAATATAAATCAAATTGTAAACCATCCACATTCGCATATCCTGCCCCTCT
  GGAAGTACCAAAAGAAAAAGAAAAGGAAAAGGTTTCCACCGCTGTGTTGTCTATTACTGCCAAAGCTAAA
  AAAAAAGAAAAAGAAAAAGAAAAAAAGGAGGAGGAGAAGATGGAAGTGGATGAAGCAGAGAAGAAGGAAG
  AGAAAGAGAAGAAAAAAGAACCTGAACCAAACTTCCAGCTGTTGGATAATCCAGCACGAGTTATGCCTGC
  CCAGCTGAAGGTCTTAAGTATGACAGAGACCTGCAGATACCAGCCTTTTAAACCACTCTCCATCGGGGGC
  ATCATAATTCTGAAAGACACCAGCGAAGACGTTGAAGAACTCGTGGAACCCGTGGCAGCACACGGCCCAA
  AGATTGAAGAGGAAGAGCAAGAGCCAGAGCCCCCAGAGCCGTTCGAGTATATCGATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227375 representing NM_027357
  Red=Cloning site Green=Tags(s)
  
  MITSAAGIISLLDEEEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYH
  LGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQR
  CLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEK
  PDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPG
  STNTGTVPGSEKDSDPMETEEKTASAVAGKTPDASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDL
  MILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEAL
  QLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTAR
  QDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRM
  EEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTP
  EQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTE
  ITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFW
  FWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAK
  KKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLSMTETCRYQPFKPLSIGG
  IIILKDTSEDVEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027357
• ORF Size: 2859 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027357.2, NP_081633.1
• RefSeq Size: 3256
• RefSeq ORF: 2862
• Locus ID: 70247
• Gene Summary: In eukaryotic cells, most proteins in the cytosol and nucleus are degraded via the ubiquitin-proteasome pathway. The 26S proteasome is a self-compartmentalizing protease comprised of approximately 31 different subunits. It contains a barrel-shaped proteolytic core complex (the 20S proteasome), capped at one or both ends by 19S regulatory complexes, which recognize ubiquitinated proteins. Protein degradation by proteasomes is the source of most antigenic peptides presented on MHC class I molecules. This gene encodes a non-ATPase subunit of the 26S proteasome. [provided by RefSeq, Jul 2008]