Cyp1a1 (NM_009992) Mouse Tagged ORF Clone

CAT#: MG227414

Cyp1a1 (GFP-tagged) - Mouse cytochrome P450 family 1 subfamily a polypeptide 1 (Cyp1a1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyp1a1
• Synonyms: AHH; AHRR; CP11; CYPIA1; P450-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227414 representing NM_009992
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCCATGTATGGACTTCCAGCCTTCGTGTCAGCCACAGAGCTGCTCCTGGCTGTCACCGTATTCT
  GCCTTGGATTCTGGGTGGTCAGAGCCACAAGAACCTGGGTTCCCAAAGGCCTGAAGACTCCACCAGGGCC
  CTGGGGCTTGCCCTTCATTGGTCACATGCTGACTGTGGGGAAGAACCCACATCTGTCACTGACACGGCTG
  AGTCAGCAGTATGGGGACGTGCTGCAGATCCGCATCGGCTCCACTCCTGTGGTGGTGCTGAGCGGCCTGA
  ACACCATCAAGCAGGCCCTGGTGAGGCAGGGAGATGACTTCAAGGGCCGGCCAGACCTCTACAGCTTCAC
  ACTTATCACTAATGGCAAGAGCATGACTTTTAACCCAGACTCTGGACCCGTGTGGGCTGCCCGCCGGCGC
  CTGGCCCAGAATGCCCTGAAGAGCTTCTCCATAGCCTCGGACCCGACGTCAGCATCCTCTTGCTACTTGG
  AGGAGCACGTGAGCAAGGAGGCTAACTATCTCGTCAGCAAACTTCAGAAGGTGATGGCAGAGGTTGGCCA
  CTTTGACCCTTACAAGTATTTGGTCGTGTCAGTAGCCAATGTCATCTGTGCCATATGCTTTGGCCAACGT
  TATGACCATGATGACCAAGAGCTGCTCAGCATAGTCAATCTGAGCAATGAGTTTGGGGAGGTTACTGGCT
  CTGGATACCCAGCTGACTTCATTCCTGTCCTCCGTTACCTGCCTAACTCTTCCCTGGATGCCTTCAAGGA
  CTTGAATGATAAGTTCTACAGCTTCATGAAGAAGTTAATCAAAGAGCACTACAGGACATTTGAGAAGGGC
  CACATCCGGGACATCACAGACAGCCTCATTGAGCATTGTCAGGACAGGAAGCTGGACGAGAATGCCAATG
  TCCAGCTGTCAGATGATAAGGTCATCACGATTGTTTTGGACCTCTTTGGAGCTGGGTTTGACACAGTCAC
  AACTGCTATCTCGTGGAGCCTCATGTACCTGGTAACCAACCCTAGGGTACAGAGAAAGATCCAGGAGGAA
  CTAGACACAGTGATTGGCAGAGATCGGCAGCCCCGGCTTTCTGACAGACCTCAGCTGCCCTATCTGGAGG
  CCTTCATTCTGGAGACCTTCCGGCATTCATCCTTCGTCCCCTTCACCATCCCCCACAGCACCACAAGAGA
  TACAAGTCTGAATGGCTTCTATATCCCCAAGGGGTGCTGTGTCTTTGTGAACCAGTGGCAGGTTAACCAT
  GACCGGGAACTGTGGGGTGACCCAAACGAGTTCCGGCCTGAAAGGTTTCTCACCCCCAGCGGCACTCTGG
  ACAAGCGCTTGAGTGAGAAGGTCACTCTCTTTGGTTTGGGCAAGCGAAAGTGCATCGGAGAGACCATTGG
  CCGATCGGAGGTCTTTCTCTTCCTGGCCATCTTGCTGCAGCAAATAGAATTTAAGGTGTCTCCAGGGGAG
  AAGGTGGATATGACTCCTACCTATGGGCTGACTTTAAAACACGCCCGCTGTGAACACTTCCAAGTGCAGA
  TGCGGTCTTCTGGTCCTCAGCATCTTCAGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227414 representing NM_009992
  Red=Cloning site Green=Tags(s)
  
  MPSMYGLPAFVSATELLLAVTVFCLGFWVVRATRTWVPKGLKTPPGPWGLPFIGHMLTVGKNPHLSLTRL
  SQQYGDVLQIRIGSTPVVVLSGLNTIKQALVRQGDDFKGRPDLYSFTLITNGKSMTFNPDSGPVWAARRR
  LAQNALKSFSIASDPTSASSCYLEEHVSKEANYLVSKLQKVMAEVGHFDPYKYLVVSVANVICAICFGQR
  YDHDDQELLSIVNLSNEFGEVTGSGYPADFIPVLRYLPNSSLDAFKDLNDKFYSFMKKLIKEHYRTFEKG
  HIRDITDSLIEHCQDRKLDENANVQLSDDKVITIVLDLFGAGFDTVTTAISWSLMYLVTNPRVQRKIQEE
  LDTVIGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTSLNGFYIPKGCCVFVNQWQVNH
  DRELWGDPNEFRPERFLTPSGTLDKRLSEKVTLFGLGKRKCIGETIGRSEVFLFLAILLQQIEFKVSPGE
  KVDMTPTYGLTLKHARCEHFQVQMRSSGPQHLQA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009992
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009992.4, NP_034122.1
• RefSeq Size: 2716
• RefSeq ORF: 1575
• Locus ID: 13076
• Gene Summary: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. [UniProtKB/Swiss-Prot Function]