Ihh (NM_010544) Mouse Tagged ORF Clone

CAT#: MG227435

Ihh (GFP-tagged) - Mouse Indian hedgehog (Ihh), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ihh
• Synonyms: HHG-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227435 representing NM_010544
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCCCAAGAGCCACCCAGACGCCTGAGTCCCCGAAGCTGTCCCAGCCACGCGCCCACCTATCAG
  CCCACCAGGCGCCCTCGCCCGCTGCTCTCCCGGGCTACCCGGCCATGTCTCCCGCCTGGCTCCGGCCCCG
  ACTGCGGTTCTGTCTGTTCCTGCTGCTGCTGCTTCTGGTGCCGGCGGCGCGGGGCTGCGGGCCGGGCCGG
  GTGGTGGGCAGCCGCCGGAGGCCGCCTCGCAAGCTCGTGCCTCTTGCCTACAAGCAGTTCAGCCCCAACG
  TGCCGGAGAAGACCCTGGGCGCCAGCGGGCGCTACGAAGGCAAGATCGCGCGCAGCTCTGAGCGCTTCAA
  AGAGCTCACCCCCAACTACAATCCCGACATCATCTTCAAGGACGAGGAGAACACGGGTGCCGACCGCCTC
  ATGACCCAGCGCTGCAAGGACCGTCTGAACTCACTGGCCATCTCTGTCATGAACCAGTGGCCTGGTGTGA
  AACTGCGGGTGACCGAAGGCTGGGATGAAGATGGCCATCACTCAGAGGAGTCTTTACACTATGAGGGCCG
  CGCGGTGGATATCACCACCTCAGACCGTGACCGAAATAAGTATGGACTGCTGGCGCGCTTAGCAGTGGAG
  GCCGGCTTCGACTGGGTGTATTACGAGTCCAAGGCCCACGTGCATTGCTCTGTCAAGTCTGAGCATTCGG
  CCGCTGCCAAGACAGGTGGCTGCTTTCCTGCCGGAGCCCAGGTGCGCCTAGAGAACGGGGAGCGTGTGGC
  CCTGTCAGCTGTAAAGCCAGGAGACCGGGTGCTGGCCATGGGGGAGGATGGGACCCCCACCTTCAGTGAT
  GTGCTTATTTTCCTGGACCGCGAGCCAAACCGGCTGAGAGCTTTCCAGGTCATCGAGACTCAGGATCCTC
  CGCGTCGGCTGGCGCTCACGCCTGCCCACCTGCTCTTCATTGCGGACAATCATACAGAACCAGCAGCCCA
  CTTCCGGGCCACATTTGCCAGCCATGTGCAACCAGGCCAATATGTGCTGGTATCAGGGGTACCAGGCCTC
  CAGCCTGCTCGGGTGGCAGCTGTCTCCACCCACGTGGCCCTTGGGTCCTATGCTCCTCTCACAAGGCATG
  GGACACTTGTGGTGGAGGATGTGGTGGCCTCCTGCTTTGCAGCTGTGGCTGACCACCATCTGGCTCAGTT
  GGCCTTCTGGCCCCTGCGACTGTTTCCCAGTTTGGCATGGGGCAGCTGGACCCCAAGTGAGGGTGTTCAC
  TGGTACCCTCAGATGCTCTACCGCCTGGGGCGTCTCTTGCTAGAAGAGAGCACCTTCCATCCACTGGGCA
  TGTCTGGGGCAGGAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010544
• ORF Size: 1347 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010544.2, NP_034674.1
• RefSeq Size: 2464
• RefSeq ORF: 1236
• Locus ID: 16147
• Gene Summary: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). [UniProtKB/Swiss-Prot Function]