Bco1 (NM_001163028) Mouse Tagged ORF Clone

CAT#: MG227449

Bcmo1 (GFP-tagged) - Mouse beta-carotene 1515'-monooxygenase (Bcmo1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bco1
• Synonyms: Bcdo; Bcdo1; Bcmo1; beta-CD; betaCMOOX; CMO1; Cmoi
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227449 representing NM_001163028
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATAATATTTGGCCAGAATAAGAAAGAACAGCTGGAGCCAGTTCAGGCCAAAGTGACAGGCAGCA
  TTCCAGCATGGCTGCAGGGGACCCTGCTCCGAAACGGGCCCGGGATGCACACAGTGGGAGAGAGCAAGTA
  CAACCATTGGTTTGATGGCCTGGCCCTTCTCCACAGTTTCTCCATCAGAGATGGGGAGGTCTTCTACAGG
  AGCAAATACCTGCAGAGTGACACCTACATCGCCAACATTGAGGCCAACAGAATCGTGGTGTCTGAGTTCG
  GAACCATGGCCTACCCGGACCCCTGCAAAAACATCTTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT
  CCCCGACTTCACAGACAACTGTCTGATCAACATCATGAAATGTGGAGAAGACTTCTATGCAACCACGGAG
  ACCAACTACATCAGGAAAATCGACCCCCAGACCCTAGAGACCTTGGAGAAGGTTGATTACCGGAAGTATG
  TGGCGGTAAACCTGGCTACCTCGCACCCTCATTATGACGAGGCTGGGAATGTCCTTAACATGGGCACATC
  CGTCGTGGACAAAGGGAGGACAAAATACGTGATATTTAAGATCCCTGCCACAGTGCCAGACAGCAAGAAG
  AAAGGGAAGAGTCCCGTGAAGCACGCGGAAGTTTTCTGCTCCATTTCCTCCCGCTCGTTGCTCTCTCCCA
  GCTACTACCACAGCTTTGGTGTCACGGAGAACTATGTGGTGTTTCTGGAGCAGCCTTTTAAGTTGGATAT
  CCTCAAGATGGCCACCGCATACATGAGGGGAGTGAGCTGGGCTTCCTGTATGTCATTCGACAGGGAGGAC
  AAGACATACATTCATATCATCGACCAGAGGACCAGGAAGCCTGTGCCTACCAAGTTCTACACAGATCCCA
  TGGTGGTCTTCCATCATGTCAATGCCTACGAGGAGGACGGCTGTGTGCTGTTTGATGTGATCGCCTATGA
  GGACAGCAGCCTCTATCAGCTCTTCTACCTGGCCAACCTGAACAAGGACTTCGAGGAGAAGTCCAGGCTG
  ACCTCAGTGCCTACCCTCAGGAGGTTTGCTGTGCCCCTCCATGTGGACAAGGATGCAGAAGTGGGCTCAA
  ATTTAGTCAAGGTGTCATCTACAACTGCAACAGCCCTGAAGGAGAAAGACGGCCATGTCTATTGCCAGCC
  CGAGGTCCTCTACGAAGGCCTAGAGCTCCCTCGGATAAATTATGCTTACAACGGGAAGCCATATCGCTAC
  ATCTTTGCAGCTGAAGTACAGTGGAGTCCAGTCCCAACCAAGATACTGAAATATGACATTCTCACAAAGT
  CCTCCTTAAAGTGGTCTGAGGAGAGCTGCTGGCCAGCAGAGCCTCTGTTTGTTCCCACGCCAGGTGCGAA
  GGATGAAGATGATGTCCCTTGCAGGCCAAGCAGAGACTGTGGAGTCCAAGGGCCCCATCGTAAATCACAT
  TGTCAGCTGAGCATAGCATGTGTGCCTGAGATCTCAGCATTTGGGAGGCAGACAGAAGACAATCAGGGGT
  CTACAGTCATTCTCAGATATACAGAGAATTTGAACTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227449 representing NM_001163028
  Red=Cloning site Green=Tags(s)
  
  MEIIFGQNKKEQLEPVQAKVTGSIPAWLQGTLLRNGPGMHTVGESKYNHWFDGLALLHSFSIRDGEVFYR
  SKYLQSDTYIANIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATTE
  TNYIRKIDPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSVVDKGRTKYVIFKIPATVPDSKK
  KGKSPVKHAEVFCSISSRSLLSPSYYHSFGVTENYVVFLEQPFKLDILKMATAYMRGVSWASCMSFDRED
  KTYIHIIDQRTRKPVPTKFYTDPMVVFHHVNAYEEDGCVLFDVIAYEDSSLYQLFYLANLNKDFEEKSRL
  TSVPTLRRFAVPLHVDKDAEVGSNLVKVSSTTATALKEKDGHVYCQPEVLYEGLELPRINYAYNGKPYRY
  IFAAEVQWSPVPTKILKYDILTKSSLKWSEESCWPAEPLFVPTPGAKDEDDVPCRPSRDCGVQGPHRKSH
  CQLSIACVPEISAFGRQTEDNQGSTVILRYTENLNY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163028
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001163028.1, NP_001156500.1
• RefSeq Size: 2532
• RefSeq ORF: 1581
• Locus ID: 63857
• Gene Summary: Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]