Agap1 (NM_001037136) Mouse Tagged ORF Clone

CAT#: MG227528

Agap1 (GFP-tagged) - Mouse ArfGAP with GTPase domain ankyrin repeat and PH domain 1 (Agap1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Agap1
• Synonyms: Centg2; Ggap1; mKIAA1099; R75364
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227528 representing NM_001037136
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTCGAGTCGGTCC
  ACCCCAACATCTACTCCATCTACGAGCTGCTGGAGCGCGTGGAGGAGCCGGTGCTGCAGAACCAGATTCG
  GGAGCACGTCATCGCCATCGAAGATGCTTTCGTGAACAGCCAGGAATGGACACTCAGCCGATCGGTACCG
  GAGCTCAAAGTGGGAATTGTGGGTAACCTGGCCAGTGGCAAGTCTGCGCTGGTGCACCGGTACCTGACAG
  GCACGTACGTGCAGGAGGAATCTCCAGAAGGTGGCAGGTTCAAGAAGGAGATTGTTGTTGATGGACAGAG
  TTATCTGCTGCTGATTAGGGATGAAGGGGGCCCCCCGGAGGCACAGTTCGCCATGTGGGTGGACGCGGTC
  ATCTTTGTCTTCAGCTTGGAGGATGAGATCAGTTTCCAGACTGTCTACCATTACTACAGCCGAATGGCCA
  ACTACAGGAACACCAGTGAGATCCCATTAGTGCTGGTGGGGACCCAGGATGCCATAAGTTCCACCAACCC
  GAGGGTCATCGATGACGTCAGGGCTCGGAAGCTCTCCAATGACCTGAAGAGGTGCACGTACTATGAGACG
  TGCGCCACCTATGGGCTCAACGTGGAGCGGGTCTTCCAGGACGTTGCCCAGAAGATCGTTGCCACAAGGA
  AGAAGCAGCAGCTATCCATAGGCCCCTGCAAGTCCTTACCCAACTCTCCAAGCCACTCGTCTGTGTGTTC
  TGCCCAAGTGTCCGCGGTACACATCAGCCAGACAAGTAACGGAGGTGGGAGTTTAAGTGACTACTCATCC
  TCTGTTCCTTCGACCCCAAGCACCAGCCAGAAAGAGCTTCGGATCGACGTCCCTCCCACTGCCAACACTC
  CAACGCCTGTCCGGAAGCAGTCCAAGCGCAGGTCCAACCTATTCACATCTAGGAAAGGGAGCGACCCAGA
  CAAAGAGAAGAAAGGCCTGGAGAGCCGTGCGGACAGCATCGGGAGCGGGCGAGCCATCCCAATTAAACAG
  GGCATGCTGCTGAAGCGAAGTGGCAAGTCACTGAACAAAGAGTGGAAAAAGAAGTACGTCACCCTGTGCG
  ACAACGGTGTGCTGACCTACCACCCCAGTTTACATGATTACATGCAGAATGTCCACGGCAAAGAGATAGA
  CCTCTTGAGGACCACTGTGAAGGTGCCGGGGAAGAGGCCGCCCCGAGCCACGTCTGCCTGTGCACCGATC
  TCCAGTCCCAAAACCAACGGCCTGGCCAAGGACATGAGCAGTTTACACATCTCACCAAATTCAGACACGG
  GGCTGGGTGACTCGGTGTGTTCCAGCCCAAGTATCTCCAGCAGCACCAGCCCCAAGCTCGACCCACCCCC
  CTCCCCCCACGCCAACAGAAAGAAGCACCGGAGGAAGAAAAGTACCAGCAACTTCAAGGCTGACGGGCTC
  TCAGGCACTGCTGAAGAGCAAGAAGAAAACTTGGAGTTTATCATTGTGTCCCTCACTGGCCAGACGTGGC
  ACTTTGAAGCCACCACATACGAGGAGCGAGACGCATGGGTCCAAGCCATCGAGAGCCAGATCCTCGCCAG
  CCTGCAGTCGTGCGAGAGCAGCAAGAATAAGTCCCGACTAACCAGCCAGAGCGAAGCCATGGCCCTGCAG
  TCCATCCGTAACATGAGAGGGAACTCGCACTGCGTGGACTGTGACACCCAAAACCCTAACTGGGCCAGTT
  TGAACTTGGGAGCCCTCATGTGTATCGAGTGCTCGGGGATCCACCGGAATCTCGGCACCCACCTCTCTCG
  GGTCAGATCGCTAGACCTGGACGACTGGCCCATGGAGCTGATCAAGGTGATGTCATCCATCGGGAATGAG
  CTGGCCAACAGCGTGTGGGAAGAGGGCAGCCAGGGACGGACAAAGCCCTCACTGGACTCCACCAGGGAAG
  AAAAGGAAAGGTGGATCCGGGCCAAGTATGAGCAGAAACTCTTCCTGGCCCCACTGCCATGCACAGAGTT
  CTCCCTGGGCCAGCAGCTGCTGCGTGCCACTGCCGAGGAAGACCTGCGGACTGTCATCCTACTTCTGGCA
  CATGGGTCCCGGGACGAGGTGAATGAGACCTGTGGGGAAGGAGACGGTCGCACGGCGCTGCACCTGGCCT
  GTCGCAAGGGCAACGTGGTCCTGGCTCAGCTGCTGATCTGGTACGGAGTGGACGTCATGGCCCGAGACGC
  CCACGGGAACACAGCACTGGCCTATGCCCGGCAGGCCTCCAGCCAGGAGTGTATTGACGTGCTTCTACAG
  TATGGCTGCCCCGACGAGCGCTTTGTGCTCATGGCTACCCCTAACCTGTCCAGGAAGAGCAACAGCAGGA
  ACAACAGTAGCGGGAGGGCGCCCAGTGTCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227528 representing NM_001037136
  Red=Cloning site Green=Tags(s)
  
  MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQNQIREHVIAIEDAFVNSQEWTLSRSVP
  ELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAV
  IFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYET
  CATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSS
  SVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQ
  GMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPI
  SSPKTNGLAKDMSSLHISPNSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGL
  SGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQ
  SIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNE
  LANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLRATAEEDLRTVILLLA
  HGSRDEVNETCGEGDGRTALHLACRKGNVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQ
  YGCPDERFVLMATPNLSRKSNSRNNSSGRAPSVI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037136
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037136.1, NP_001032213.1
• RefSeq Size: 9422 bp
• RefSeq ORF: 2415 bp
• Locus ID: 347722
• Cytogenetics: 1 D