Prkcz (NM_008860) Mouse Tagged ORF Clone

CAT#: MG227620

Prkcz (GFP-tagged) - Mouse protein kinase C zeta (Prkcz) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prkcz
• Synonyms: AI098070; aPKCzeta; C80388; nPKC-zeta; Pkcz; R74924; zetaPKC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227620 representing NM_008860
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGCAGGACGGACCCCAAGATGGACCGGAGCGGCGGCCGCGTCCGTCTGAAGGCGCACTACGGCG
  GGGACATCCTGATTACCAGCGTGGATGCCATGACAACATTCAAGGACCTCTGTGAGGAAGTGCGAGACAT
  GTGTGGCCTGCACCAGCAGCACCCACTCACCCTCAAGTGGGTGGACAGTGAAGGTGACCCTTGTACTGTG
  TCCTCACAGATGGAGCTGGAGGAGGCCTTCCGCCTGGTCTGTCAGGGCAGGGACGAAGTGCTCATCATTC
  ATGTTTTCCCAAGCATCCCAGAGCAGCCGGGCATGCCTTGTCCTGGAGAAGACAAGTCCATCTACCGCCG
  TGGAGCCAGAAGATGGAGGAAGCTGTACCGAGCCAACGGCCACCTCTTCCAAGCCAAGCGCTTTAACAGG
  GGAGCGTACTGCGGCCAGTGCAGCGAAAGGATATGGGGTCTCTCGAGGCAGGGCTACAGGTGCATCAACT
  GCAAGCTGCTGGTCCATAAACGCTGCCACGTCCTCGTCCCGCTGACCTGCAGGAGGCATATGGATTCTGT
  CATGCCTTCCCAAGAGCCTCCAGTAGATGACAAGAACGATGGTGTAGACCTTCCTTCAGAAGAAACTGAT
  GGAATTGCTTATATTTCTTCATCTCGGAAACATGATAATATCAAAGATGATTCTGAGGACCTTAAGCCTG
  TCATCGATGGGGTGGATGGGATCAAAATCTCTCAGGGGCTGGGGCTGCAAGACTTCGACCTCATCAGAGT
  CATCGGGCGTGGAAGCTATGCCAAGGTCCTCCTGGTGCGGTTGAAGAAAAACGACCAGATTTACGCCATG
  AAGGTGGTAAAGAAGGAGCTTGTCCACGACGACGAGGATATCGACTGGGTGCAGACAGAGAAACATGTGT
  TTGAGCAGGCGTCCAGCAACCCCTTCCTGGTTGGCTTACACTCCTGCTTCCAGACAACGAGCCGGTTGTT
  CCTGGTCATCGAGTATGTCAATGGCGGGGACCTCATGTTCCACATGCAGAGGCAGAGAAAACTTCCAGAG
  GAGCATGCCAGGTTCTATGCTGCTGAGATCTGTATCGCTCTCAACTTCTTGCATGAGAGGGGGATCATCT
  ACCGGGACCTAAAACTGGACAACGTCCTCCTTGATGCCGACGGACACATTAAGCTGACGGACTACGGCAT
  GTGCAAGGAAGGTCTAGGCCCCGGTGATACAACAAGCACTTTTTGTGGAACCCCGAACTATATCGCCCCC
  GAAATCCTGCGAGGAGAAGAGTACGGGTTCAGCGTGGACTGGTGGGCACTGGGTGTCCTTATGTTTGAGA
  TGATGGCTGGGCGCTCCCCCTTTGACATCATCACGGACAACCCTGACATGAACACTGAAGACTACCTTTT
  CCAAGTTATCCTGGAAAAGCCAATCCGGATTCCCCGTTTCCTGTCTGTCAAGGCCTCACACGTCTTAAAA
  GGATTTTTAAATAAGGATCCCAAAGAGAGGCTTGGCTGCCGGCCACAGACTGGGTTTTCCGACATCAAGT
  CTCATGCTTTCTTCTGCAGCATAGACTGGGACCTGCTGGAAAAGAAGCAGACCCTGCCTCCCTTCCAGCC
  CCAGATCACAGATGACTATGGCCTGGACAACTTTGACACGCAGTTCACCAGCGAGCCTGTGCAGCTGACC
  CCAGATGATGAGGACGTCATAAAGAGGATCGACCAGTCCGAATTTGAAGGCTTTGAGTACATCAACCCAC
  TTCTGCTGTCTGCTGAGGAGTCCGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227620 representing NM_008860
  Red=Cloning site Green=Tags(s)
  
  MPSRTDPKMDRSGGRVRLKAHYGGDILITSVDAMTTFKDLCEEVRDMCGLHQQHPLTLKWVDSEGDPCTV
  SSQMELEEAFRLVCQGRDEVLIIHVFPSIPEQPGMPCPGEDKSIYRRGARRWRKLYRANGHLFQAKRFNR
  GAYCGQCSERIWGLSRQGYRCINCKLLVHKRCHVLVPLTCRRHMDSVMPSQEPPVDDKNDGVDLPSEETD
  GIAYISSSRKHDNIKDDSEDLKPVIDGVDGIKISQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAM
  KVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPE
  EHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAP
  EILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLK
  GFLNKDPKERLGCRPQTGFSDIKSHAFFCSIDWDLLEKKQTLPPFQPQITDDYGLDNFDTQFTSEPVQLT
  PDDEDVIKRIDQSEFEGFEYINPLLLSAEESV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008860
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008860.2
• RefSeq Size: 4283
• RefSeq ORF: 1779
• Locus ID: 18762