Cyb5a (NM_025797) Mouse Tagged ORF Clone

CAT#: MR200799

Cyb5 (Myc-DDK-tagged) - Mouse cytochrome b-5 (Cyb5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyb5a
• Synonyms: 0610009N12Rik; Cyb5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200799 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCAGTCAGACAAGGATGTGAAGTACTACACCCTGGAAGAGATTCAGAAGCACAAAGACAGCA
  AGAGCACCTGGGTGATCCTGCATCATAAGGTGTACGATCTGACCAAGTTTCTCGAAGAGCATCCTGGTGG
  AGAAGAAGTCCTAAGAGAGCAAGCTGGGGGTGATGCTACCGAGAATTTTGAGGACGTCGGGCACTCTACG
  GATGCACGAGAACTGTCCAAAACATACATCATCGGGGAACTCCATCCAGATGACAGATCAAAGATAGCCA
  AGCCTTCGGATACTCTTATCACTACTGTGGAGTCTAATTCCAGTTGGTGGACCAACTGGGTGATCCCAGC
  CATCTCAGCCCTGGCTGTAGCTCTGATGTATCGCCTCTACATGGCAGAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200799 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGQSDKDVKYYTLEEIQKHKDSKSTWVILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHST
  DARELSKTYIIGELHPDDRSKIAKPSDTLITTVESNSSWWTNWVIPAISALAVALMYRLYMAED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025797
• ORF Size: 405 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025797.1, NM_025797.2, NM_025797.3, NM_025797.4, NP_080073.1
• RefSeq Size: 749 bp
• RefSeq ORF: 405 bp
• Locus ID: 109672
• MW: 15.2 kDa
• Gene Summary: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation. [UniProtKB/Swiss-Prot Function]