Vps28 (NM_025842) Mouse Tagged ORF Clone

CAT#: MR202545

Vps28 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 28 (yeast) (Vps28)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vps28
• Synonyms: 1110014J03Rik; CIIA; D730005C08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202545 representing NM_025842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCACGGGATCCCAGCTACTCCTGGTGTTGGAGCCCCTGGGAACAAGCCGGAGCTGTATGAGGAAG
  TAAAGCTCTACAAGAATGCTCGGGAGCGGGAGAAGTATGACAACATGGCAGAGCTCTTTGCCGTGGTGAA
  GACGATGCAGGCCCTGGAGAAGGCGTACATCAAGGACTGTGTCACCCCCAATGAGTACACTGCAGCCTGC
  TCCAGGCTCCTGGTCCAGTACAAAGCTGCCTTCCGACAGGTCCAAGGCTCAGAGATCAGCTCCATTGATG
  AATTTTGCCGAAAGTTCAGACTGGACTGCCCACTTGCTATGGAGAGGATCAAAGAGGACCGGCCCATCAC
  TATCAAAGACGACAAGGGCAATCTCAACCGCTGCATTGCAGATGTTGTTTCGCTCTTCATTACAGTCATG
  GACAAGCTGCGTCTGGAGATCCGTGCCATGGACGAGATTCAGCCAGACCTGCGGGAGCTGATGGAGACAA
  TGCACAGAATGAGCCACCTGCCTCCAGACTTCGAGGGCCGCCAGACAGTCAGCCAGTGGCTGCAGACCCT
  GAGTGGTATGTCGGCCTCTGACGAGCTGGATGACTCTCAAGTTCGCCAGATGCTCTTCGATCTGGAGTCC
  GCTTACAACGCCTTTAACCGCTTCCTACACGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202545 representing NM_025842
  Red=Cloning site Green=Tags(s)
  
  MFHGIPATPGVGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVTPNEYTAAC
  SRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVM
  DKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLES
  AYNAFNRFLHA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025842
• ORF Size: 663 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025842.1, NM_025842.2, NM_025842.3, NM_025842.4, NP_080118.1
• RefSeq Size: 944
• RefSeq ORF: 666
• Locus ID: 66914
• MW: 25.5 kDa
• Gene Summary: This gene encodes a protein which is thought to be a subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]