Ube2s (NM_133777) Mouse Tagged ORF Clone

CAT#: MR202594

Ube2s (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2S (Ube2s)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube2s
• Synonyms: 0910001J09Rik; 6720465F12Rik; AA409170; E2-EPF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202594 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCAATGTGGAGAATCTGCCGCCCCACATCATCCGCCTAGTGTACAAGGAGGTGACAACACTGA
  CGGCAGACCCACCTGATGGCATTAAAGTCTTCCCCAATGAGGAGGATCTCACAGACCTGCAGGTTACCAT
  CGAGGGCCCTGAAGGGACTCCCTATGCTGGAGGTCTGTTCCGTATGAAGCTCCTACTGGGGAAGGACTTC
  CCTGCCTCCCCACCCAAGGGCTACTTCCTGACTAAAATCTTCCACCCAAATGTGGGCCCCAATGGCGAGA
  TCTGTGTCAATGTGCTCAAGAGGGACTGGACGGCTGAATTGGGCATACGACATGTGCTGCTGACCATCAA
  GTGCCTGCTGATCCACCCTAACCCAGAGTCTGCACTCAATGAGGAGGCAGGCCGCCTGCTTTTGGAGAAT
  TATGAAGAGTATGCTGCCCGGGCCCGTCTGCTCACAGAAATCCATGGGGGTGCATGCAGCACCAGCAGCG
  GGAGGGCTGAGGCTACCCAAGACCTGGCCAGTGGGGCTTCAGCCTCCTCTGCTGACCCTATGATCCCAGG
  GGTCCTAGGGGGAGCTGAGGGTCCCATGGCCAAGAAACATGCAGGTGAGCGAGATAAGAAGCTGGCAGCC
  AAGAAAAAGTTGGACAAGAAGCGAGCACTGAGGCGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202594 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDF
  PASPPKGYFLTKIFHPNVGPNGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLEN
  YEEYAARARLLTEIHGGACSTSSGRAEATQDLASGASASSADPMIPGVLGGAEGPMAKKHAGERDKKLAA
  KKKLDKKRALRRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133777
• ORF Size: 672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133777.1, NM_133777.2, NP_598538.1
• RefSeq Size: 988 bp
• RefSeq ORF: 672 bp
• Locus ID: 77891
• MW: 24.2 kDa