Cldn8 (NM_018778) Mouse Tagged ORF Clone

CAT#: MR202643

Cldn8 (Myc-DDK-tagged) - Mouse claudin 8 (Cldn8)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cldn8
• Synonyms: AI648025
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202643 representing NM_018778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCTACGCTCTTCAAATGGCTGCACTGGTGCTTGGTGGTGTTGGCATGGTGGGCACGGTGGCTG
  TGACTATCATGCCTCAGTGGAGAGTGTCTGCCTTCATCGAAAGTAACATTGTGGTGTTTGAGAACCGCTG
  GGAAGGCTTGTGGATGAATTGTATGAGGCATGCCAACATCAGAATGCAGTGCAAGGTCTACGACTCCCTG
  CTGGCTCTTAGTCCAGACCTCCAGGCATCCCGAGGACTGATGTGTGCTGCGTCCGTCTTGGCTTTCTTGG
  CTTTCATGACAGCCATCCTCGGAATGAAGTGCACCAGATGCACGGGGGACGATGAGAACGTGAAGAGCCG
  CATCTTGCTGACAGCCGGAATCATCTTCTTCATCACCGGCTTGGTTGTGCTCATCCCTGTCAGCTGGGTT
  GCCAATTCCATCATCAGAGACTTCTACAACCCACTGGTGGATGTGGCCCTAAAGCGCGAGCTGGGAGAAG
  CCCTCTACATAGGCTGGACCACAGCGCTGGTGCTGATCGCTGGAGGAGCACTGTTCTGTTGTGTGTTTTG
  TTGTACTGAAAGGAGCAACAGTTACAGGTACTCGGTACCATCCCATCGCACCACTCAACGGAGTTTCCAC
  GCCGAAAAGAGATCTCCGAGCATATACTCCAAAAGTCAGTATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202643 representing NM_018778
  Red=Cloning site Green=Tags(s)
  
  MATYALQMAALVLGGVGMVGTVAVTIMPQWRVSAFIESNIVVFENRWEGLWMNCMRHANIRMQCKVYDSL
  LALSPDLQASRGLMCAASVLAFLAFMTAILGMKCTRCTGDDENVKSRILLTAGIIFFITGLVVLIPVSWV
  ANSIIRDFYNPLVDVALKRELGEALYIGWTTALVLIAGGALFCCVFCCTERSNSYRYSVPSHRTTQRSFH
  AEKRSPSIYSKSQYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018778
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018778.1, NM_018778.2, NM_018778.3, NP_061248.1
• RefSeq Size: 2368
• RefSeq ORF: 678
• Locus ID: 54420
• MW: 25.4 kDa
• Gene Summary: This intronless gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a paracellular cation barrier. [provided by RefSeq, Aug 2010]