Atp6v1e1 (NM_007510) Mouse Tagged ORF Clone

CAT#: MR202657

Atp6v1e1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit E1 (Atp6v1e1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp6v1e1
• Synonyms: 2410029D23Rik; Atp6e; Atp6e2; Atp6v1e; D6Ertd385e; p31; Vma4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202657 representing NM_007510
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCAGCGATGCAGATGTACAGAAGCAGATTAAGCACATGATGGCTTTCATTGAACAAGAAGCCA
  ATGAGAAAGCAGAAGAAATAGATGCAAAGGCAGAAGAAGAGTTCAACATTGAGAAAGGTCGCCTTGTGCA
  AACGCAAAGACTGAAGATTATGGAATACTACGAGAAGAAAGAAAAGCAGATTGAGCAGCAGAAGAAAATT
  CAGATGTCCAACTTGATGAATCAAGCAAGGCTCAAAGTCCTCAGAGCAAGGGATGACCTCATCACTGATC
  TGCTAAATGAGGCAAAGCAGAGACTCAGTAAGGTGGTAAAAGATACGACCCGTTACCAAGTGCTGCTGGA
  TGGGCTGGTCCTTCAGGGCTTGTACCAGCTGCTGGAGCCTCGAATGATCGTGCGTTGCAGAAAACAAGAT
  TTCCCTTTGGTGAAGGCCGCAGTACAAAAAGCAATCCCTATGTATAAAATTGCTACCAAAAAGGATGTTG
  ATGTCCAGATTGACCAGGAGGCCTACCTGCCTGAGGAAATAGCTGGTGGCGTTGAGATCTATAATGGGGA
  TCGCAAGATAAAGGTTTCCAACACCCTGGAAAGCCGGCTGGACCTGATAGCCCAGCAGATGATGCCAGAA
  GTCCGTGGAGCCTTGTTTGGTGCAAATGCCAATAGGAAGTTTCTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202657 representing NM_007510
  Red=Cloning site Green=Tags(s)
  
  MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKI
  QMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQD
  FPLVKAAVQKAIPMYKIATKKDVDVQIDQEAYLPEEIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPE
  VRGALFGANANRKFLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007510
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007510.2, NP_031536.2
• RefSeq Size: 1219
• RefSeq ORF: 681
• Locus ID: 11973
• MW: 26.2 kDa
• Gene Summary: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. [UniProtKB/Swiss-Prot Function]