Cd79b (NM_008339) Mouse Tagged ORF Clone

CAT#: MR202725

Cd79b (Myc-DDK-tagged) - Mouse CD79B antigen (Cd79b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd79b
• Synonyms: B29; Ig-beta; Igb; Igbeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202725 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACACTGGTGCTGTCTTCCATGCCCTGCCACTGGCTGTTGTTCCTGCTGCTGCTCTTCTCAGGTG
  AGCCGGTACCAGCAATGACAAGCAGTGACCTGCCACTGAATTTCCAAGGAAGCCCTTGTTCCCAGATCTG
  GCAGCACCCGAGGTTTGCAGCCAAAAAGCGGAGCTCCATGGTGAAGTTTCACTGCTACACAAACCACTCA
  GGTGCACTGACCTGGTTCCGAAAGCGAGGGAGCCAGCAGCCCCAGGAACTGGTCTCAGAAGAGGGACGCA
  TTGTGCAGACCCAGAATGGCTCTGTCTACACCCTCACTATCCAAAACATCCAGTACGAGGATAATGGTAT
  CTACTTCTGCAAGCAGAAATGTGACAGCGCCAACCATAATGTCACCGACAGCTGTGGCACGGAACTTCTA
  GTCTTAGGATTCAGCACGTTGGACCAACTGAAGCGGCGGAACACACTGAAAGATGGCATTATCTTGATCC
  AGACCCTCCTCATCATCCTCTTCATCATTGTGCCCATCTTCCTGCTACTTGACAAGGATGACGGCAAGGC
  TGGGATGGAGGAAGATCACACCTATGAGGGCTTGAACATTGACCAGACAGCCACCTATGAAGACATAGTG
  ACTCTTCGGACAGGGGAGGTAAAGTGGTCGGTAGGAGAGCATCCAGGCCAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202725 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATLVLSSMPCHWLLFLLLLFSGEPVPAMTSSDLPLNFQGSPCSQIWQHPRFAAKKRSSMVKFHCYTNHS
  GALTWFRKRGSQQPQELVSEEGRIVQTQNGSVYTLTIQNIQYEDNGIYFCKQKCDSANHNVTDSCGTELL
  VLGFSTLDQLKRRNTLKDGIILIQTLLIILFIIVPIFLLLDKDDGKAGMEEDHTYEGLNIDQTATYEDIV
  TLRTGEVKWSVGEHPGQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008339
• ORF Size: 687 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008339.1, NM_008339.2, NM_008339.3, NP_032365.1
• RefSeq Size: 1232 bp
• RefSeq ORF: 687 bp
• Locus ID: 15985
• MW: 25.7 kDa
• Gene Summary: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2015]