Bcl2l1 (NM_009743) Mouse Tagged ORF Clone
CAT#: MR202824L4
- LentiORF®
Lenti ORF clone of Bcl2l1 (mGFP-tagged) - Mouse BCL2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein
"NM_009743" in other vectors (6)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Bcl2l1 |
Synonyms | Bcl(X)L; bcl-x; Bcl-XL; bcl2-L-1; Bcl2l; BclX |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR202824).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_009743 |
ORF Size | 699 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009743.4, NP_033873.3 |
RefSeq Size | 2371 bp |
RefSeq ORF | 702 bp |
Locus ID | 12048 |
UniProt ID | Q64373, Q5HZH3 |
Cytogenetics | 2 H1 |
Gene Summary | This gene encodes a member of the Bcl-2 family of apoptosis regulators. The encoded protein is localized to the inner and outer mitochondrial membranes and regulates the programmed cell death pathway during development and tissue homeostasis. This protein binds to voltage-dependent anion channels in the outer mitochondrial membrane to facilitate the uptake of calcium ions. Mice embryos lacking this gene survived for two weeks and exhibited cell death of immature hematopoietic cells and neurons. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207993 | Bcl2l1 (untagged) - Mouse BCL2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein, (10ug) |
USD 540.00 |
|
MR202824 | Bcl2l1 (Myc-DDK-tagged) - Mouse BCL2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein |
USD 240.00 |
|
MG202824 | Bcl2l1 (GFP-tagged) - Mouse Bcl2-like 1 (Bcl2l1) |
USD 260.00 |
|
MR202824L1 | Lenti ORF clone of Bcl2l1 (Myc-DDK-tagged) - Mouse BCL2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein |
USD 540.00 |
|
MR202824L2 | Lenti ORF clone of Bcl2l1 (mGFP-tagged) - Mouse BCL2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein |
USD 440.00 |
|
MR202824L3 | Lenti ORF clone of Bcl2l1 (Myc-DDK-tagged) - Mouse BCL2-like 1 (Bcl2l1), nuclear gene encoding mitochondrial protein |
USD 440.00 |
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