Unc119 (NM_011676) Mouse Tagged ORF Clone

CAT#: MR202960

Unc119 (Myc-DDK-tagged) - Mouse unc-119 homolog (C. elegans) (Unc119)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Unc119
• Synonyms: D11Bhm52; HRG4; MRG4; Rg4; Rtg4; Unc119h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202960 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTGAAGAAAGGCGGCGGCGGGACCGGGTCGGGGGCGGAGCCCGTTCCAGGGGCCTCGAACCGGA
  GCGCGGAGCCCACGCGGGAGCCTGGGGCGGAAGCTGAGTCCGGGTCCGAGTCGGAGCCGGAGCCAGGCCC
  GGGGCCCAGGCTGGGGCCGCTGCAGGGCAAGCAGCCCATCGGGCCGGAGGATGTGCTGGGGCTGCAGCGG
  ATCACGGGTGACTACCTGTGCTCCCCTGAGGAAAATATCTACAAGATTGACTTCGTCAGGTTCAAGATCC
  GTGACATGGACTCAGGGACTGTCCTTTTTGAAATCAAGAAGCCCCCTGTTTCGGAACGGTTGCCCATCAA
  CCGGCGGGACCTGGACCCCAATGCAGGGCGCTTTGTTCGCTACCAGTTCACACCTGCCTTCCTCCGCCTA
  AGGCAGGTGGGAGCCACGGTGGAGTTCACAGTGGGAGACAAGCCGGTCAACAACTTCCGCATGATCGAGA
  GGCACTACTTTCGAAACCAGCTCCTCAAAAGCTTCGACTTCCACTTTGGCTTCTGCATCCCCAGCAGCAA
  GAACACCTGTGAGCACATCTATGACTTCCCTCCTCTCTCCGAGGAGCTAATCAGTGAGATGATTCGTCAC
  CCGTATGAGACCCAGTCTGACAGCTTCTACTTCGTGGATGACCGGCTGGTGATGCATAACAAAGCAGACT
  ATTCCTACAGTGGGACACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202960 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKVKKGGGGTGSGAEPVPGASNRSAEPTREPGAEAESGSESEPEPGPGPRLGPLQGKQPIGPEDVLGLQR
  ITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRDLDPNAGRFVRYQFTPAFLRL
  RQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRH
  PYETQSDSFYFVDDRLVMHNKADYSYSGTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011676
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011676.1, NM_011676.2, NM_011676.3, NP_035806.1
• RefSeq Size: 1319 bp
• RefSeq ORF: 723 bp
• Locus ID: 22248
• MW: 27 kDa
• Gene Summary: The protein encoded by this gene is multifunctional, affecting trafficking of transducin in rod photoreceptors, interacting with src-type tyrosine kinases through SH2 and SH3 interacting domains, and aiding the uptake of bacteria through endocytosis. In addition, the encoded protein acts as a lipid-binding chaperone to help localize some myristoylated proteins correctly. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]