Trh (NM_009426) Mouse Tagged ORF Clone

CAT#: MR203300

Trh (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone (Trh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trh
• Synonyms: Pro-TRH; Trf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203300 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGACCTTGGCTGATGATGGCTCTGGCTTTGATCTTCGTGCTAACTGGTATCCCCAAATCCTGCG
  CCTTGCTGGAAGCAGCCCAGGAGGAAGGTGCTGTGACTCCTGACCTTCCAGGCCTGGAGAAAGTCCAGGT
  CCGGCCAGAACGTCGATTCTTGAGGAAAGACCTCCAGCGTGTGCGAGGGGACCTTGGTGCTGCCTTAGAT
  TCCTGGATCACAAAACGCCAGCATCCGGGCAAAAGGGAGGAGAAGTTGGAAGACGAAGCCGAAGAGAGGG
  GAGATTTGGGAGAAGTGGGAGCCTGGAGACCCCACAAACGACAGCACCCTGGCCGACGTGCCAACCAAGA
  CAAGGATTCATGGTCAGATGAGGGGGACAGTGACTGGCTGCCCCCGTCCTGGCTACCAGATTTCTTTCTG
  GATTCCTGGTTCTCAGATGCCCCCCAAGTCAAGCGGCAGCACCCTGGCAGGCGATCCTTCCCCTGGATGG
  AGTCTGATGTCACCAAGAGGCAGCATCCAGGCCGGAGGTTCATAGATCCTGAGCTCCAAAGAAGCTGGGA
  AGAAACAGAGGGAGAGGAGGGTGGCTTAATGCCTGAGAAACGTCAGCATCCTGGCAAAAGGGCAGTGGGT
  CACCCTTGTGGGCCCCAGGGGATTTGTGGCCAAACAGGCCTGCTCCAGCTTCTAGGTGACCTGAGTAGGG
  GCCAGGAGACCCTGGCAAAGCAAACCCCACAACTGGAAGCCTGGGTCAGGGAACCTCTGGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203300 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQGPWLMMALALIFVLTGIPKSCALLEAAQEEGAVTPDLPGLEKVQVRPERRFLRKDLQRVRGDLGAALD
  SWITKRQHPGKREEKLEDEAEERGDLGEVGAWRPHKRQHPGRRANQDKDSWSDEGDSDWLPPSWLPDFFL
  DSWFSDAPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEETEGEEGGLMPEKRQHPGKRAVG
  HPCGPQGICGQTGLLQLLGDLSRGQETLAKQTPQLEAWVREPLEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009426
• ORF Size: 768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009426.1, NM_009426.2, NM_009426.3, NP_033452.2
• RefSeq Size: 1400
• RefSeq ORF: 771
• Locus ID: 22044
• MW: 29.1 kDa
• Gene Summary: This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013]